Summary
A 2-chlorobenzoic acid (2-CBA) utilizing strain ofPseudomonas aeruginosa (B16) has been isolated from soil by enrichment with 2-CBA. The trait for utilization of 2-CBA as sole source of carbon and energy was lost spontaneously and by treatment with growth-limiting concentration of mitomycin C. Genes coding for 2-CBA catabolism are transferrable to 2-CBA− variants through conjugation.
Résumé
Une souche dePseudomonas aeruginosa (B16) utilisant l'acide 2-chlorobenzoïque (2-CBA) a été isolée du sol par enrichissement sur le 2-CBA. L'aptitude à utiliser le 2-CBA comme seule source de carbone et d'énergie a été perdue spontanément et par traitement avec une concentration limitant la croissance de mitomycine-C. Les gènes codant pour le catabolisme du 2-CBA sont transférables aux variants 2-CBA− par conjugaison.
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References
Alexander, M. 1981 Biodegradation of chemicals of environmental concern.Science 211, 132–138.
Chakrabarty, A. M. 1972 Genetic basis of biodegradation of salicylate inPseudomonas.Journal of Bacteriology 112, 815–823.
Chakrabarty, A. M. 1976 Plasmids inPseudomonas.Annual Review of Genetics 10, 7–30.
Dorn, E., Hellwig, M., Reineke, W. &Knackmuss, H. J. 1974 Isolation and characterization of 3-chlorobenzoic acid degradingPseudomonas.Archives of Microbiology 99, 61–70.
Ghosal, D., You, I. S., Chatterjee, D. K. &Chakrabarty, A. M. 1985 Plasmids in degradation of chlorinated aromatic compounds. InPlasmids in Bacteria, eds. Helinski, D., Cohen, S. N., Clewell, D. B., Jackson, D. and Kollaender, A., Vol. 30, pp. 667–686. New York: Plenum Press.
Johnston, H. W., Briggs, G. G. &Alexander, M. 1972 Metabolism of 3-chlorobenzoic acid by a Pseudomonad.Soil Biology and Biochemistry 4, 287–296.
Novick, R. P. 1969 Extrachromosomal inheritance in bacteria.Bacteriological Reviews 33, 210–235.
Rangnekar, V. M. 1988 Variation in ability ofPseudomonas B13 cultures to utilise metal chlorobenzoate is associated with tandem amplification and deamplification of DNA.Journal of Bacteriology 170, 1907–1912.
Reineke, W. &Knackmuss, H. J. 1979 Construction of halogramatic utilizing bacteria.Nature (London)277, 385–386.
Reineke, W., Wessels, S. W., Rubio, M. A., Latorre, J., Schwein, U. S., Schmidt, E., Schlomann, M. &Knackmuss, H. J. 1982 Degradation of monochlorinated aromatic compounds following transfer of genes coding chlorocatechol metabolism.FEMS Microbiology Letters 14, 291–294.
Singh, H. 1987 Biochemical and Genetic Studies on Degradation of Chlorinated Aromatic Compounds byPseudomonas sp. M.Sc. Thesis, Punjab Agricultural University, Ludhiana, India.
Will, J. J., Twee, V. D., Kak, L. I. B. &Debent, J. A. M. 1987 Reductive dechlorination of 2,4-dichlorobenzoic to 4-chlorobenzoate and hydrolytic dehalogenation of 4-chloro-, 4-bromo- and 4-iodo-benzoates byAlcaligenes denitrificans NTB-1.Applied and Environmental Microbiology 53, 810–815.
Wong, C. L. &Dunn, N. W. 1974 Transmissable plasmid coding for the degradation of benzoate and m-toluate inPseudomonas putida (arvilla) mt-2.Genetics Research 23, 227–232.
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Singh, H., Kahlon, R.S. Conjugative plasmid coding for the metabolism of 2-chlorobenzoic acid byPseudomonas aeruginosa . World J Microbiol Biotechnol 5, 255–258 (1989). https://doi.org/10.1007/BF01741850
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DOI: https://doi.org/10.1007/BF01741850