Summary
Cladosporium resinae QM 7998 produced high activities of extracellular and constitutive β-glucosidase when grown on a variety of sugars or cellulose. Starch and ribose induced enzyme synthesis several fold.Cladosporium resinae could utilize agricultural waste residues for growth and β-glucosidase production. The initial pH of the medium had a marked effect on enzyme prowduction and optimum pH was between 4.0 and 5.0 depending on the assay method. Mixed culturing ofC. resinae with yeasts, viz.Saccharomyces cerevisiae andCandida utilis, increased the β-glucosidase production while that with other fungi decreased the enzyme yield. Theβ- glucosidase preparation fromC. resinae significantly increased the saccharification of rice and wheat straw (untreated or delignified) withTrichoderma reesei QM 9414 cellulase preparation.
Résumé
Cladosporium resinae QM 7998 produit des concentrations élevées de β-glucosidase tant extracellulaire que constitutive lorsqu'elle croît sur une variété de sucres ou sur la cellulose. On a trouvé que l'amidon et le ribose augmentent de plusieurs fois la quantité d'enzyme synthétisée.Cladosporium resinae peut utiliser des résidus agricoles pour sa croissance et pour la production de β-glucosidase. Le pH initial du milieu exerce un effet marqué sur la production d'enzyme et le pH optimum est compris entre 4.0 et 5.0 selon les conditions de l'essai. La croissance mixte deCladosporium resinae avec diverses levures, notammentSaccharomyces cerevisiae etCandida utilis, augmente la production de β-glucosidase tandis que celle avec d'autres moisissures diminue le rendement en enzyme. La β-glucosidase deCladosporium resinae augmente de manière significative la saccharification des pailles de riz et de froment (non-traitées ou délignifiées) traités par la cellulase deTrichoderma reesei QM 9414.
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Ahluwalia, A., Gupta, J.K., Vadehra, D.V. et al. Production of β-glucosidase byCladosporium resinae . World J Microbiol Biotechnol 5, 205–215 (1989). https://doi.org/10.1007/BF01741845
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DOI: https://doi.org/10.1007/BF01741845