Degradation of different hydrocarbons and production of biosurfactant byPseudomonas aeruginosa isolated from coastal waters

  • Mohammad Shafeeq
  • D. Kokub
  • Z. M. Khalid
  • A. M. Khan
  • Kauser A. Malik
Research Papers

Summary

A bacterium isolated from an oil spillage sample and identified asPseudomonas aeruginosa degraded hexadecane and heptadecane by 47% and 58% with 9% and 12% of total carbon from the respective substrates being liberated as CO2. With octadecane and nonadecane as substrates, 73% and 60% were biodegraded while 27% and 25% of total carbon was evolved as CO2, respectively. Production of biosurfactant by this bacterium was studied using hexadecane 5% (v/v) as substrate. The surface tension of spent culture medium, as well as the supernatant, was 30 mN/m compared to 71 mN/m for water. When the supernatant was mixed with hexadecane (1∶5, v/v) a stable emulsion was formed which deteriorated only by 10% after one month.

Keywords

Surface Tension Total Carbon Hexadecane Biosurfactants Carbone 

Dégradation de différents hydrocarbures et production de biosurfactants parPseudomonas aeruginosa isolé d'eaux côtières

Résumé

Une bactérie, isolée d'un échantillon d'hydrocarbures épandus, et identifiée commePseudomonas aeruginosa, dégrade l'hexaet l'heptadécane à raison respective de 47 et de 58% du carbone total avec 9 et 12% des substrats respectifs libérés sous la forme de CO2. Avec l'octa- et le nonadécane comme substrats, la biodégradation atteint respectivement 73 et 60% avec 27 et 25% du carbone total transformé en CO2. On a étudié la production de biosurfactant par cette bactérie en utilisant l'hexadécane à 5% (v/v) comme substrat. La tension superficielle de la liqueur mixte, comme celle de son surnageant atteint 30 mN/m par comparaison à 71 mN/m pour l'eau. Lorsque le surnageant est mélangé à l'hexadécane (1∶5, v/v), on forme une émulsion stable qui ne se détériore que de 10% au bout d'un mois.

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Copyright information

© Oxford University Press 1989

Authors and Affiliations

  • Mohammad Shafeeq
    • 1
  • D. Kokub
    • 1
  • Z. M. Khalid
    • 2
  • A. M. Khan
    • 1
  • Kauser A. Malik
    • 2
  1. 1.Hydrocarbon Development Institute of PakistanIslamabadPakistan
  2. 2.Soil Biology DivisionNuclear Institute for Agriculture and BiologyFaisalabadPakistan

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