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Mutants ofPleurotus florida deficient in sporulation

  • Marilyn Yu
  • Shu -Ting Chang
Research Papers

Summary

Inheritable sporulation-deficient dikaryons were obtained through pairing compatible monokaryons. The morphological character of centralized stipes of sporulation-deficient fruit bodies was remarkable and enable them to be distinguished from sporulating ones. Light and electron microscopy showed neither spores nor sterigmata on the surface of most basidia. However, in rare cases, some basidia, always clustering in a number of a few to tens, had one abortive spore on each basidium. No mature spores were found. Genetic analysis of sporulation deficiency was carried out by test-crosses. Results suggest that sporulation-deficient mutant inP. florida is rather complex and might be controlled by two recessive genes. The recovery of four mating types in spores obtained from the dikaryotic fruit body used for test-crosses, and the presence of four nuclei on the apex of basidia of sporulation-deficient strain indicated that the process of meiosis had occurred in this sporulation-deficient mutant.

Keywords

Microscopy Electron Microscopy Genetic Analysis Microscopie Optique Rare Case 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Mutants de Pleurotus florida déficients en sporulation

Résumé

Des dikaryons à sporulation déficiente héritable ont été obtenus en accouplant des monokaryons compatibles. Le caractère morphologique des tiges centralisées des corps fruitaux déficients en sporulation est remarquable et permet de les distinguer de ceux sporulants. La microscopie optique ou électronique ne montre mi spores ni stérigmates sur la surface de la plupart des basidia. Toutefois, dans quelques rares cas, quelques basidia, toujours agglutinés par groupe de quelques unités à quelques dizaines d'unités, ont une spore avortée sur chaque basidium. On ne trouve pas de spores mûres. L'analyse génétique de la déficience en sporulation a été réalisée par des tests de croisements. Les résultats suggèrent que la mutation de déficience en sporulation chezP. florida est plutôt complexe et pourrait bien être contrôlée par deux gènes récessifs. L'obtention de quatre types d'accouplement chez les spores obtenues du corps fruital dikaryotique utilisé pour les tests de croisements, et la présence de quatre noyaux dans l'apex des basidia de la souche déficiente en sporulation, suggère que le processus de méiose se passe bien chez le mutant déficient en sporulation.

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Copyright information

© Oxford University Press 1989

Authors and Affiliations

  • Marilyn Yu
    • 1
  • Shu -Ting Chang
    • 1
  1. 1.Department of BiologyThe Chinese University of Hong KongNew TerritoriesHong Kong

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