Cancer Immunology, Immunotherapy

, Volume 33, Issue 6, pp 395–397 | Cite as

Interleukin-6 does not support interleukin-2 induced generation of human lymphokine-activated killer cells

  • Hans-G. Klingemann
  • Elaine Wong
Original articles


The activity of lymphokine-activated killer (LAK) cells is supported by various cytokines. The objective of this study was to see if recombinant interleukin-6 (IL-6) either alone or in combination with interleukin-2 (IL-2) has any effect on the generation of LAK cells. Peripheral blood mononuclear cells of healthy donors were cultured for 4 or 6 days with both cytokines either alone or in combination. LAK activity against K562 and natural killer-resistant Daudi cells was assessed by a 4-h and an 18-h51Cr-release assay at various effector to target ratios. IL-6 alone in increasing concentrations did not induce LAK cell activity. Neither additive nor synergistic effects of IL-6 with IL-2 were observed. Immunofluorescence analysis with phycoerythrin-conjugated anti-CD56 antibody demonstrated that IL-6 could not maintain or increase the number of CD56-positive cells over a 6-day culture period. These results suggest that IL-6 does not support LAK cell generation by itself or increase LAK cell activity in combination with IL-2.

Key words

Interleukin-6 LAK cells 


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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Hans-G. Klingemann
    • 1
  • Elaine Wong
    • 1
  1. 1.Terry Fox Laboratory, British Columbia Cancer Agency, Division of HematologyUniversity of British ColumbiaVancouverCanada

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