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Interleukin-6 does not support interleukin-2 induced generation of human lymphokine-activated killer cells

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Summary

The activity of lymphokine-activated killer (LAK) cells is supported by various cytokines. The objective of this study was to see if recombinant interleukin-6 (IL-6) either alone or in combination with interleukin-2 (IL-2) has any effect on the generation of LAK cells. Peripheral blood mononuclear cells of healthy donors were cultured for 4 or 6 days with both cytokines either alone or in combination. LAK activity against K562 and natural killer-resistant Daudi cells was assessed by a 4-h and an 18-h51Cr-release assay at various effector to target ratios. IL-6 alone in increasing concentrations did not induce LAK cell activity. Neither additive nor synergistic effects of IL-6 with IL-2 were observed. Immunofluorescence analysis with phycoerythrin-conjugated anti-CD56 antibody demonstrated that IL-6 could not maintain or increase the number of CD56-positive cells over a 6-day culture period. These results suggest that IL-6 does not support LAK cell generation by itself or increase LAK cell activity in combination with IL-2.

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Authors and Affiliations

  1. Terry Fox Laboratory, British Columbia Cancer Agency, Division of Hematology, University of British Columbia, 601 West 10th Avenue, V5Z 1L3, Vancouver, British Columbia, Canada

    Hans-G. Klingemann & Elaine Wong

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  1. Hans-G. Klingemann
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  2. Elaine Wong
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Klingemann, HG., Wong, E. Interleukin-6 does not support interleukin-2 induced generation of human lymphokine-activated killer cells. Cancer Immunol Immunother 33, 395–397 (1991). https://doi.org/10.1007/BF01741600

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  • DOI: https://doi.org/10.1007/BF01741600

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