Summary
The number of bone marrow cells in C3H/He mice was reduced 3–4 days after treatment with 130 mg/kg intraperitoneal 5-fluorouracil (5-FU). Higher rates of spontaneous proliferation and natural killer (NK) activity, accompanied by an increase in asialoGM1-positive cells, were observed in treated mice. When sizofiran at a dose of 200 µg/animal was intramuscularly injected after 5-FU treatment, the rates of proliferation and NK activity of bone marrow cells were higher than with 5-FU alone. The cell number was not influenced by sizofiran alone. These results indicate that all precursors of the various mature cell types (including NK cells) differentiate and regenerate rapidly to replace cells damaged by 5-FU treatment, and that sizofiran has the potential to assist this recovery. These results suggest that administration of sizofiran after chemotherapy may be useful in cancer patients.
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Cohen SA, Tsung S, Doerr RJ, Goldrosen MH (1990) Role of asialo-GM1 positive liver cells from athymic nude or polyinosinic-polycytidylic acid-treated mice in suppressing colon-derived experimental hepatic metastasis. Cancer Res 50: 18 342
Hansson M, Kiessling R, Anderson B (1981) Human fetal thymus and bone marrow contain target cells for natural killer cells. Eur J Immunol 11: 84
Hansson M, Beran M, Anderson B, Kiessling R (1982) Inhibition of in vitro granulopoiesis by autologous allogeneic human NK cells. J Immunol 129: 126
Herberman RB, Ortaldo JR (1981) Natural killer cells: their role in defenses against diseases. Science 214: 24
Ho MK, Springer TA (1982) Mac-2, a novel 32,000M r mouse macrophage subpopulation-specific antigen defined by monoclonal antibodies. J Immunol 128: 1221
Kalland T (1990) Regeneration of natural killer progenitors. Studies with a novel immunomodulator with distinct effects at the precursor level. J Immunol 144: 4472
Komatsu N, Okubo S, Kikumoto S, Kimura K, Saito S (1969) Host mediated antitumor action of schizophyllan, a glucan produced bySchizophillum commune. Jpn J Cancer Res (Gann) 60: 137
Marcel RMB, Boggs SS, Herberman RB, Hiserodt JC (1990) The generation of natural killer (NK) cells from NK precursor cells in rat long-term bone marrow cultures. J Exp Med 172: 303
Mercurio AM, Schwarting GA, Robbins PW (1984) Glycolipids of the mouse peritoneal macrophage—alterations in amount and surface exposure of specific glycolipid species occur in response to inflammation and tumoricidal activation. J Exp Med 160: 1114
Migliorati G, Cannarile L, Herberman RB, Bartocci A, Stanley ER, Riccardi C (1987) Role of interleukin 2 (IL-2) and hemopoietin-1 (H-1) in the generation of mouse natural killer (NK) cells from primitive bone marrow precursors. J Immunol 138: 3618
Mitani M, Ariga T, Matsuo T, Asano T, Saito G (1980) Anti-tumor effect of schizophyllan, an an immunomodulator on syngeneic tumors. Int J Immunopharmacol 2: 174
Moor MAS, Stolfi RL, Martin DS (1990) Hematologic effects of interleukine-1b, granulocyte colony-stimulating factor, and granulocyte-macrophage colony-stimulating factor in tumor-bearing mice treated with fluorouracil. J Natl Cancer Inst 82: 1031
Nibbering PH, Van de Gevel JS, Van Furth R (1990) A cell-ELISA for the quantification of adherent murine macrophages and the surface expression of antigens. J Immunol Methods 131: 25
Norisuye T, Yanaki T, Fujita H (1980) Triple helix of aSchizophyllum commune polysaccharide in aqueous solution. J Polym Sci: Physics Edit 18: 547
Sato M, Kaji R, Urata M, Yoshida H, Yanagawa T, Miyamoto K, Azuma M, Furumoto N, Saito M (1988) Monoclonal antibody to the streptococcal preparation OK-432: tissue OK-432 localization and analysis of interaction between OK-432 and macrophages or NK cells in human salivary adenocarcinoma-bearing nude mice given OK-432. J Biol Response Mod 7: 212
Sugawara I, Lee KC, Wong M (1984) Schizophillan(SPG)-treated macrophages and anti-tumor activities against syngeneic and allogeneic tumor cells: I. Characteristics of SPG-treated macrophages. Cancer Immunol Immunother 16: 137
Suzuki R, Handa K, Itoh K, Kumagai K (1983) Natural killer cells as a responder to interleukin 2 (IL-2): I. Proliferative response and establishment of cloned cells. J Immunol 130: 981
Suzuki R, Suzuki S, Igarashi M, Kumagai K (1986) Induction of interleukin 3 but not interleukin 2 or interferon production in the syngeneic mixed reaction. J Immunol 137: 1564
Talmadge JE, Meyers KM, Prieur DJ, Starkey JR (1980) Role of NK cells in tumor growth and metastasis inbeige mice. Nature 284: 622
Tsuchiya Y, Igarashi M, Suzuki R, Kumagai K (1988) Production of colony-stimulating factor by tumor cells and the factor-mediated induction of suppressor cells. J Immunol 141: 699
Tsuchiya Y, Igarashi M, Inoue M, Kumagai K (1989) Cytokine-related immunomodulating activities of an anti-tumor glucan, sizofiran (SPG). J PharmacobioDyn 12: 616
Uchida A, Micksche M (1981) In vitro augmentation of natural killing activity by OK-432. Int J Immunopharmacol 3: 365
Uchida A, Yagita M, Sugiyama H, Hoshino T, Moor M (1984) Strong natural killer (NK) cell activity in bone marrow of myeloma patients: accelerated maturation of bone marrow NK cells and their interaction with other bone marrow cells. Int J Cancer 34: 375
Young JC, Mule JJ, Rosenberg SA (1986) Muline lymphokine activated killer (LAK) cells; phenotypic characterization of the rat longterm bone marrow cultures. J Exp Med 172: 303
Young LHY, Klavinskis LS, Oldstone MBA, Young JDE (1989) In vivo expression of perforin by CD8 positive lymphocytes during an acute viral infection. J Exp Med 169: 2159
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Tsuchiya, Y., Matsutani, M., Inoue, M. et al. Effect of a glucan, sizofiran, on natural-killer activity of 5-fluorouracil-treated murine bone marrow cells. Cancer Immunol Immunother 34, 17–23 (1991). https://doi.org/10.1007/BF01741319
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DOI: https://doi.org/10.1007/BF01741319