, Volume 61, Issue 1, pp 14–16 | Cite as

Usefulness of Y-body study on bone marrow smears to demonstrate the origin of fibroblast stromal cells in allogeneic bone marrow transplantation

  • J. M. Ribera
  • E. Feliu
  • R. Arriols
  • A. Grañena
  • J. L. Vives-Corrons
  • C. Rozman
Original article


The value of Y-body study for assessment of stromal cell engraftment was analyzed in 25 patients submitted to allogeneic bone marrow transplantation (BMT) (sex-matched in 12 cases and sex-mismatched in 13). The study was performed weekly on bone marrow smears until day + 35, and the results were compared with those obtained in a control group of 20 patients submitted to autologous BMT (12 males and 8 females). Engraftment of haemopoietic cells was documented in all cases. The results of Y-body study on the recipients' fibroblast cells showed a pattern identical to that observed prior to BMT, independent of donor's sex. On the other hand, there were no differences between allogeneic and autologous BMT recipients in regard to percentage of Y-body positive cells. These results indicate that in allogeneic BMT there is no engraftment of the fibroblastic component of bone marrow stroma.

Key words

Allogeneic bone marrow transplantation Fibroblast stromal engraftment Y-Body 


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  1. 1.
    Allen TD, Dexter TM (1984) The essential cells of the hemopoietic microenvironment. Exp Hematol 12: 517–521Google Scholar
  2. 2.
    Bentley SA, Knutsen T, Whang-Peng J (1982) The origin of the hematopoietic microenvironment in continuous bone marrow culture. Exp Hematol 10: 367–372Google Scholar
  3. 3.
    Casperson T, Zech L, Johansson C, Lindsten J, Hulsten M (1970) Fluorescent staining of heteropichnotic chromosome regions in human interphase nuclei. Exp Cell Res 61: 472–474Google Scholar
  4. 4.
    Coccia PF, Krivit W, Cervenka L (1980) Successful bonemarrow transplantation for infantile malignant osteopetrosis. N Engl J Med 302: 701–708Google Scholar
  5. 5.
    Dexter TM, Allen TD, Lajtha LG (1977) Conditions controlling the proliferation of haemopoietic stem cells in vitro. J Cell Physiol 91: 335–344Google Scholar
  6. 6.
    Friedenstein AJ, Ivan-Smolenski AA, Chailakhjan RK (1976) Origin of bone marrow stromal mechanocytes in radiochimeras and heterotopic transplants. Exp Hematol 6: 440–444Google Scholar
  7. 7.
    Gartner SM, Kaplan HS (1980) Long-term culture of human bone marrow cells. Proc Natl Acad Sci USA 77: 4756–4761Google Scholar
  8. 8.
    Golde DW, Hocking WG, Quang SG (1980) Origin of human bone marrow fibroblasts. Br J Haematol 44: 183–187Google Scholar
  9. 9.
    Gordon MY (1988) The origin of stromal cells in patients treated by bone marrow transplantation. Bone Marrow Transpl 3: 247–251Google Scholar
  10. 10.
    Gordon MY (1988) Extracellular matrix of the marrow microenvironment. Br J Haematol 70: 1–4Google Scholar
  11. 11.
    Keating A, Singer JW, Killen PD (1982) Donor origin of the in vitro haematopoietic microenvironment after bone marrow transplantation in man. Nature 298: 280–283Google Scholar
  12. 12.
    Laver J, Jhanwar SC, O'Reilly RJ, Castro-Malaspina H (1987) Host origin of the human hematopoietic microenvironment following allogeneic bone marrow transplantation. Blood 70: 1966–1968Google Scholar
  13. 13.
    Lichtman MA (1981) The ultrastructure of hematopoietic microenvironment. A review. Exp Hematol 9: 391–410Google Scholar
  14. 14.
    Ma DD, Da WM, Purvis Smith S (1987) Chromosomal analysis of bone marrow stromal fibroblasts in allogeneic HLA compatible sibling bone marrow transplantation. Leuk Res 11: 661–663Google Scholar
  15. 15.
    Marshall MJ, Nisbet NW, Evans S (1984) Donor origin of the in vitro microenvironment after transplantation in mice. Experimentia 40: 385–389Google Scholar
  16. 16.
    Piersma AH, Ploemacher RE, Brockbanck KGM (1983) Transplantation of bone marrow fibroblastoid stromal cells in mice via the intravenous route. Br Haematol 54: 285–290Google Scholar
  17. 17.
    Raskind WH, Singer JW, Morgan CA, Fialkow PJ (1988) Host origin of marrow stromal cells obtained from marrow transplant recipients and transformed in vitro by Simian virus 40. Exp Hematol 16: 827–830Google Scholar
  18. 18.
    Ribera JM, Feliu E, Rozman C (1988) Y-body study in bone marrow precursors, peripheral blood cells and alveolar macrophages for demonstration of haemopoietic engraftment in allogeneic bone marrow transplantation. Eur J Haematol 40: 424–429Google Scholar
  19. 19.
    Simmonds PJ, Przepiorka D, Thomas ED, Torok-Storb B (1987) Host origin of bone marrow stromal cells following allogeneic bone marrow transplantation. Nature 328: 429–432Google Scholar
  20. 20.
    Singer JW, Keating A, Wight TN (1985) The human hematopoietic microenvironment. In: Hoffbrand AV (ed) Recent advances in haematology. Churchill Livingstone, Edinburgh, pp 1–24Google Scholar
  21. 21.
    Wilson FD, Greenberg BR, Konrad ON (1978) Cytogenetic studies on bone marrow fibroblasts from a male-female hematopoietic chimera: evidence that stromal elements in human transplant recipients are of host type. Transplantation 25: 87–88Google Scholar
  22. 22.
    Zipori D (1989) Stromal cells from the bone marrow: evidence for a restrictive role in regulation of hemopoiesis. Eur J Haematol 42: 225–232Google Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • J. M. Ribera
    • 1
  • E. Feliu
    • 1
  • R. Arriols
    • 1
  • A. Grañena
    • 1
  • J. L. Vives-Corrons
    • 1
  • C. Rozman
    • 1
  1. 1.Postgraduate School of Haematology “Farreras Valentí” and Laboratori Central d'Hematologia Hospital ClínicUniversity of BarcelonaBarcelonaSpain

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