Summary
The concept of ex vivo expansion of the human progenitor cell population was tested in this study. In the presence of interleukin-3 and -6 we compared the abilities of various liquid culture conditions of human blood-derived hematopoietic progenitor cells to yield a suitable condition for the expansion of blood progenitor cells. Although the best result was obtained in the gas-permeable bag culture system, the enhancement effect (maximum of 3.8-fold for granulocyte/macrophage colony-forming units and 3.0-fold for erythroid burst-forming units) lasted for only a short period (< 6 d). The results of this study are disappointing for clinical use, and attempts with currently available technology appear to be limited in their potential.
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Abbreviations
- BFU-E:
-
Erythroid burst-forming units
- CFU-GM:
-
granulocyte/macrophage colony-forming units
- Epo:
-
recombinant human erythropoietin
- G-CSF:
-
recombinant human granulocytecolony-stimulating factor
- IFN-γ:
-
recombinant human interferongamma
- IL-3:
-
recombinant human interleukin-3
- IL-6:
-
recombinant human interleukin-6
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Takaue, Y., Abe, T., Kawano, Y. et al. Combination of recombinant cytokines fails to produce ex vivo expansion of human blood hematopoietic progenitor cells. Ann Hematol 64, 217–220 (1992). https://doi.org/10.1007/BF01738299
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DOI: https://doi.org/10.1007/BF01738299