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Journal of Muscle Research & Cell Motility

, Volume 13, Issue 6, pp 685–691 | Cite as

Time course of rise of muscle stiffness at onset of contraction induced by photorelease of ATP

  • K. Horiuti
  • T. Sakoda
  • K. Yamada
Papers

Summary

Isometric contraction (9–10°C) of skinned fibres from rat psoas muscle was elicited by photorelease of ATP (1.3–1.4 mM), and force (F), in-phase (I) and quadrature (Q) stiffness were monitored. The sinusoidal length change for the stiffness measurement was ≈ 0.09% fibre length and 200–1000 Hz. On photolysis,F andI initially fell, and then rose. The half-time of the rise ofI was shorter than that ofF. Contrary to the previous assumption,Q rose even earlier thanI. Although the time courses of the rise ofF andI were greatly affected by the presence of phosphate ion (Pi; 4 mM), its effect onQ was modest. We propose the following hypothesis: the rigor crossbridges, after binding ATP, initially enter a state characterized by highQ with lowI and none or littleF, and then, releasing Pi during passing the state with the increasedI, reach the final force generating state.

Keywords

Phosphate Fibre Length Isometric Contraction Length Change Psoas Muscle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Chapman & Hall 1992

Authors and Affiliations

  • K. Horiuti
    • 1
  • T. Sakoda
    • 1
  • K. Yamada
    • 1
  1. 1.Department of PhysiologyOita Medical UniversityOitaJapan

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