Journal of Muscle Research & Cell Motility

, Volume 13, Issue 4, pp 464–472 | Cite as

Effects of ethylene glycol and calcium on the kinetics of contraction induced by photo-release of low concentrations of ATP in rat psoas muscle fibres

  • T. Sakoda
  • K. Horiuti


To induce isometric contraction in the absence of Ca2+ (10mm EGTA), low concentrations (130 μM) of ATP were photoreleased from caged ATP in skinned fibres from rat psoas muscle at 15–16° C. The magnitude of contraction was independent of the concentration of EGTA (1–30mm). Each isometric transient (i) was paired with another (s) obtained under the same conditions but with 0.4% muscle stretch to elevate the rigor force before photolysis. The algebraic difference (d) betweeni ands was assumed to represent detachment of the crossbridges. The time course of force development (f) by the reattached crossbridges could be estimated by subtracting an appropriately scaledd fromi (ors). Ethylene glycol (20% in solvent) reduced the magnitude and the rate of rise off, although it scarcely affectedd, suggesting that ethylene glycol inhibited reattachment of the crossbridges but not their detachment. The presence of Ca2+ (50 μM) increased the magnitude off, but did not affect its time course (130 μM ATP). Detachment,d, was not influenced by Ca2+ in terms of both extent and rate. The effect of Ca2+ in the presence of ethylene glycol was indistinguishable from that in its absence. Ethylene glycol did not seem to substantially affect the extent of Ca-regulation on the contractile activity.


Calcium Ethylene Cage Muscle Fibre Ethylene Glycol 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bremel, R. D. &Weber, A. (1972) Cooperation within actin filament in vertebrate skeletal muscle.Nature, New Biology 238, 97–101.Google Scholar
  2. Brenner, B. (1988) Effect of Ca2+ on crossbridge turnover kinetics in skinned single rabbit psoas fibers: implications for regulation of muscle contraction.Proc. Natl. Acad. Sci. USA 85, 3265–9.PubMedGoogle Scholar
  3. Crank, J. (1975)The Mathematics of Diffusion, 2nd edn, Oxford: Clarendon Press.Google Scholar
  4. Dantzig, J. A., Goldman, Y. E., Luttmann, M. L., Trentham, D. R. &Woodward, S. K. A. (1989) Binding of caged ATP diastereoisomers to rigor crossbridges in glycerol-extracted fibres of rabbit psoas muscle.J. Physiol. 418, 61P (Abstract).Google Scholar
  5. Ferenczi, M. A., Homsher, E. &Trentham, D. R. (1984) The kinetics of magnesium adenosine triphosphate cleavage in skinned muscle fibres of the rabbit.J. Physiol. 352, 575–99.PubMedGoogle Scholar
  6. Godt, R. E. (1974) Calcium-activated tension of skinned muscle fibres of the frog: dependence on magnesium adenosine triphosphate concentration.J. Gen. Physiol. 63, 722–39.Google Scholar
  7. Goldman, Y. E., Hibberd, M. G., Mccray, J. A. &Trentham, D. R. (1982) Relaxation of muscle fibres by photolysis of caged ATP.Nature 300, 701–5.PubMedGoogle Scholar
  8. Goldman, Y. E., Hibberd, M. G. &Trentham, D. R. (1984a) Relaxation of rabbit psoas muscle fibres from rigor by photochemical generation of adenosine-5′-triphosphate.J. Physiol. 354, 577–604.PubMedGoogle Scholar
  9. Goldman, Y. E., Hibberd, M. G. &Trentham, D. R. (1984b) Initiation of active contraction by photogeneration of adenosine-5′-triphosphate in rabbit psoas muscle fibres.J. Physiol. 354, 605–24.PubMedGoogle Scholar
  10. Horiuti, K., Somlyo, A. V., Goldman, Y. E. &Somlyo, A. P. (1989) Kinetics of contraction initiated by flash photolysis of caged adenosine triphosphate in tonic and phasic smooth muscle.J. Gen. Physiol. 94, 769–81.PubMedGoogle Scholar
  11. Horiuti, K., Sakoda, T., Takei, M. &Yamada, K. (1992) Effects of ethylene glycol on kinetics of contraction on flash photolysis of caged ATP in rat psoas muscle fibres.J. Muscle Res. Cell Motil. 13, 199–205.PubMedGoogle Scholar
  12. Kaplan, J. H., Forbush III, B. &Hoffman, J. F. (1978) Rapid photolytic release of adenosine 5′-triphosphate from a protected analogue: utilization by the Na:K pump of human red blood cell ghosts.Biochemistry 17, 1929–35.PubMedGoogle Scholar
  13. Kushmerick, M. J. &Podolsky, R. J. (1969) Ionic mobility in muscle cells.Science 166, 1297–8.PubMedGoogle Scholar
  14. Maruyama, T., Kometani, K. &Yamada, K. (1989) Modification of the contractile properties of rabbit skeletal muscle by ethylene glycol.J. Biochem. 105, 1009–13.PubMedGoogle Scholar
  15. Millar, N. C. &Homsher, E. (1990) The effect of phosphate and calcium on force generation in glycerinated rabbit skeletal muscle fibres.J. Biol. Chem. 265, 20234–40.PubMedGoogle Scholar
  16. Moisescu, D. G. (1976) Kinetics of reaction in calcium-activated skinned muscle fibres.Nature 262, 610–13.PubMedGoogle Scholar
  17. Poole, K. J. V., Rapp, G., Maéda, Y. &Goody, R. S. (1988) The time course of changes in the equatorial diffraction patterns from different muscle types on photolysis of caged-ATP.Adv. Exp. Med. Biol. 226, 391–404.PubMedGoogle Scholar
  18. Reuben, J. P., Brandt, P. W., Berman, M. &Grundfest, H. (1971) Regulation of tension in the skinned crayfish muscle fibre. I. Contraction and relaxation in the absence of Ca (pCa > 9).J. Gen. Physiol. 57, 385–407.PubMedGoogle Scholar
  19. Sakoda, T., Horiuti, K. &Yamada, K. (1991) Effects of ethylene glycol and calcium on force transients upon photogeneration of low concentrations of ATP in rat psoas fibres.Jpn. J. Physiol. 41, S285 (Abstract).Google Scholar
  20. Stephenson, D. G. &Williams, D. A. (1981) Calcium-activated force responses in fast- and slow-twitch skinned muscle fibres of the rat at different temperatures.J. Physiol. 317, 281–302.PubMedGoogle Scholar
  21. Walker, J. W. &Moss, R. L. (1990) Effects of Ca2+ on crossbridge transitions measured by photogeneration of Pi and ATP.Biophys. J. 57, 538a (Abstract).Google Scholar

Copyright information

© Chapman & Hall 1992

Authors and Affiliations

  • T. Sakoda
    • 1
  • K. Horiuti
    • 1
  1. 1.Department of PhysiologyOita Medical UniversityOitaJapan

Personalised recommendations