Summary
The membrane phenotype of AML clonogenic cells (L-CFU) was analyzed in 19 AML patients using an in vitro culture technique after a complement-mediated lysis assay employing a panel of six monoclonal antibodies (McAb) -HLA-DR, FMC56 (CD9), FMC27 (CD9), CD14, CD15, CD41a-. Our results show that L-CFU has a heterogeneous but immature phenotype lacking on the expression of differentiation antigens (CD14, CD15, CD41a). In addition, we observed that the L-CFU phenotype is different from that of the whole blast cell population. Interestingly, L-CFU showed a higher expression of HLA-DR antigens with respect to their progeny. Upon analyzing whether the L-CFU phenotype was related to both the morphological and immunological features of AML blast cells, it was observed that, while there is no correlation with the FAB classification, there was a partial relationship between the immunological phenotype of AML blast cells and that of L-CFU. Accordingly, the more immature AML cases showed a more differentiated L-CFU phenotype (HLA-DR+, CD9+, FMC27+) when compared with cases with a more mature blast cell phenotype. These results suggest that those AML cases with a relatively immature myeloblastic phenotype may arise from a progenitor cell that has undergone partial differentiation and that is unable to acquire myeloid differentiation antigens, while those AML cases with mature blast cells might emerge from a very early L-CFU that has the capacity to undergo a greater degree of differentiation.
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References
Sabbath KD, Griffin JD (1985) Clonogenic cells in acute myeloblastic leukaemia. Scand J Haematol 35:251–256
Griffin JD, Löwenberg B (1986) Clonogenic cells in acute myeloblastic leukemia. Blood 6:1185–1195
Griffin JD, Davis R, Nelson DA, Davey FR, Mayer RJ, Schiffer C, McIntyre OR, Bloomfield CD (1986) Use of surface marker analysis to predict outcome of adult acute myeloblastic leukemia. Blood 68:1232–1241
San Miguel JF, Gonzalez M, Cañizo MC, Anta JP, Zola H, Lopez Borrasca A (1986) Surface marker analysis in acute myeloid leukaemia and correlation with FAB classification. Br J Haematol 64:547–560
Ball ED (1990) Immunophenotyping of acute myeloid leukemia cells. Clin Lab Med 10:721–736
Drexler HG, Minowada J (1986) The use of monoclonal antibodies for the identification and classification of acute myeloid leukemias. Leuk Res 10:279–290
Robak T, Dowding C, Garewal G, Hibbin JA, Th'ng KH, Goldman JM (1986) Antigenic determinants on myeloid leukaemia colony-forming cells resemble those of normal myeloid progenitor cells and differ from those of circulating blast cells. Br J Haematol 64:133–148
Kabral A, Bradstock K, Grimsley P, Favaloro EJ (1988) Immunophenotype of clonogenic cells in myeloid leukemia. Leuk Res 12:51–59
Lange B, Ferrero D, Pessano S, Palumbo A, Faust J, Meo P, Rovera G (1984) Surface phenotype of clonogenic cells in acute myeloid leukemia defined by monoclonal antibodies. Blood 64:693–700
Sabbath KD, Ball ED, Larcom P, Davis RB, Griffin JD (1985) Heterogeneity of clonogenic cells in acute myeloblastic leukemia. J Clin Invest 75:746–753
Bennett JM, Catovsky D, Daniel MT, Flandrin G, Galton DAG, Gralnick H, Sultan C (1976) Proposals for the classification of the acute leukaemias. Br J Haematol 33:451–458
Bennett JM, Catovsky D, Daniel MT, Flandrin D, Galton DAG, Gralnick H, Sultan C (1985) Proposed revised criteria for the classification of acute myeloid leukemia. Ann Intern Med 103:626–629
Minden MD, Buick RN, McCulloch EA (1979) Separation of blast cells and T-lymphocyte progenitors in the blood of patients with acute myeloblastic leukemia. Blood 54:186–195
Buick RN, Till JE, McCulloch EA (1977) Colony assay for proliferative blast cells circulating in myeloblastic leukemia. Lancet 1:862–863
Schlossman S, Chess L, Humphreys R, Strominger JL (1976) Distribution of la-like molecules on the surface of normal and leukemic human cells. Proc Natl Acad Sci USA 73:1288–1292
Griffin JD, Ritz Y, Nadler LM, Schlossman SF (1981) Expression of myeloid differentiation antigens on normal and malignant myeloid cells. J Clin Invest 68:932–941
Zola H, McNamara PJ, Beckman IGER, Brooks DA, Macardle PJ, Bradley J, Goodall K, Skinner J, Colins R, Berndt MC (1984) Monoclonal antibodies against antigens of the human platelet surface: preparation and properties. Pathology 16:73–78
Zola H, Potter A, Neoh SH, Juttner CA, Haylock DN, Rice AM, Favaloro EJ, Kabral A, Bradstock KF (1987) Evaluation of a monoclonal IgM antibody for purging of bone marrow for autologous transplantation. Bone Marrow Transplant 1:297–301
Todd RF, Nadler LM, Schlossman SI (1981) Antigen on human monocytes identified by monoclonal antibodies. J Immunol 126:1435–1442
Majdic O, Liszka K, Lutz D, Knapp W (1981) Myeloid differentiation antigen defined by a monoclonal antibody. Blood 58:1127–1133
Vainchenker W, Deschamps JF, Bastin JM, Guichard J, Titeux M, Breton Gorius J, McMichael A (1982) Two monoclonal antiplatelet antibodies as markers of human megakaryocyte maturation. Immunofluorescent staining and platelet peroxidase detection in megakaryocyte colonies and in vivo cells from normal and leukemic patients. Blood 59:514–521
Swart K, Hagemeijer A, Lowënberg B (1982) Acute myeloid leukemia colony growth: differences of colony forming cells in PHA-supplemented and standard leukocyte feeder cultures. Blood 59:816–821
Howell AL, Stukel TA, Bloomfield CD, Ball ED (1992) Predictive value of flow cytometric analyses of blast cells in assessing the phenotype of the leukemia colony-forming cell (L-CFC) population in acute myloid leukemia. Bone Marrow Transplant 10:261–266
Löwenberg B, Bauman JGJ (1985) Further results in understanding the subpopulation structure of AML: clonogenic cells and their progeny identified by differentiation markers. Blood 66:1225–1232
Griffin JD, Larcom P, Schlossman SF (1983) Use of surface markers to identify a subset of acute myelomonocytic leukemia cells with progenitor cell properties. Blood 62:1300–1303
Fialkow PJ, Tinger JW, Adamson JW, Vaidya K, Dow LW, Ochs J, Moohr JW (1981) Acute nonlymphocytic leukemia: Heterogeneity of stem cell origin. Blood 57:1068–1073
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del Cañizo, M.C., Almeida, J., Miguel, J.F.S. et al. The phenotype of L-CFU and its correlation with the immunological characteristics of the blast cell population in AML. Ann Hematol 68, 233–236 (1994). https://doi.org/10.1007/BF01737422
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DOI: https://doi.org/10.1007/BF01737422