Abstract
J001, an acylated poly-(1,3)-galactoside purified from the membrane ofKlebsiella pneumoniae, associates selectively with macrophages via the binding to CD11b and CD14 molecules. Inflammatory foci known to recruit macrophages could thus be imaged with technetium-99m labelled J001. This study aims to define the optimal scintigraphic protocol for99mTc-J001 imaging and to evaluate the specificity of J001 scans. A dose range study was conducted in rabbits with immunological arthritis using six different specific activities ranging from 370 to 11840 MBq·mg−1 while the intravenously injected activity was constant (37 MBq) Radiochemical purity for each preparation was documented together with the in vivo stability of the99mTc-J001 complex using exclusion-diffusion radioHPLC of serum collected 1 h after radiopharmaceutical administration. Scintigraphic images were recorded at 2, 3 and 4 h and analysed using indexes calculated from regions of interest. Specificity of the macrophage imaging was assessed by comparison with scans obtained after administration of99mTcO4 − or99mTc-albumin nanocolloids. A protocol of plasma transfusion was also used to inject99mTc-J001 after complete removal of radioactive colloids likely to be generated during the labelling. For the higher specific activities (5920 and 11840 MBq·mg−1), radiochemical purity degradation and in vitro99mTc transchelation were noted. To prevent transchelation and99mTc bond hydrolysis likely to impair imaging specificity, 1480 MBq·mg−1 corresponding to 25 µg injected J001 was found to be the optimal usable specific activity. Results obtained with the various tracers support the hypothesis that macrophage targeting is the main factor involved in the J001 imaging of arthritis.
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Hmama Z, Kouassi E, Panaye G, Delassan S, Normier G, Binz H, Revillard JP. Binding of a bacterial acylpoly(1,3)galactoside to human blood leucocytes.Scand J Immunol 1992; 36: 11–20.
Hmama Z, Normier G, Kouassi E, Flacher M, Binz H, Revillard JP. Binding of a membrane proteoglycan fromKlebsiella pneumoniae and its derivatives to human leukocytes.Immunobiology 1992; 186: 183–198.
Hmama Z, Mey A, Normier G, Binz H, Revillard JP. CD14 and CD11b mediate serum-independent binding to human monocytes of an acylpolygalactoside isolated fromKlebsiella pneumoniae.Infect Immun 1994; 62: 1520–1527.
Perin F, Pittet JC, Hoffschir D, Normier G, Binz H, Le Pape A. Scintigraphic potentials of J001X acylated poly-galactoside for imaging inflammatory lesions in pigs.Nucl Med Biol 1993; 20: 963–971.
Le Pape A. The targeting of macrophages with bacterial proteoglycans: a new strategy for the scintigraphic imaging of tumors and inflammatory lesions.Eur J Nucl Med 1989; 15: 424.
Kouassi E, Hmama Z, Lina G, Vial J, Faure-Barba F, Normier G, Binz H, Revillard JP. Activation of human monocyte chemiluminescence response by acylpoly(1,3)galactosides derived fromKlebsiella pneumoniae.J Leuk Biol 1992; 52: 529–535.
Mey A, Ponard D, Colomb M, Normier G, Binz H, Revillard JP. Acylation of the lipid A region of aKlebsiella pneumoniae LPS controls the alternative pathway activation of human complement.Mol Immunol 1994; 31: 1239–1246.
Pittet JC. Ciblage in vivo des macrophages par une sonde d'origine bactérienne, pour l'imagerie scintigraphique des foyers inflammatoires, infectieux et tumoraux du système lymphatique. PhD Thesis, Tours, France, 1994.
Diot P, Le Pape A, Nolibe D, Normier G, Binz H, Revillard JP, Lasfargues G, Lavandier M, Lemarie E. Scintigraphy with J001X, aKlebsiella membrane glycolipid, for the early diagnosis of chronic berylliosis: results from an experimental model.Br J Ind Med 1992; 49: 359–364.
Diot P, Lemarie E, Baulieu JL, Pascal S, Vaillant L, Revillard JP, Binz H, Normier G, Le Pape A. Scintigraphy with J001 macrophage targeting glycolipopeptide. A new approach for sarcoidosis imaging.Chest 1992; 102: 670–676.
Diot P, Diot E, Lemarie E, Guilmot JL, Baulieu JI, Asquier E, Valat C, Delarue A, Le Pape A. Imaging of pulmonary disease in scleroderma using J001X scintigraphy.Thorax 1994; 49: 504–508.
Muller C, Le Pape A, Musset M, Baulieu JL, Delarue A, Binz H, Vaillant L, Lorette G. Scintigraphy with 99m technetium-J001X acylated poly-galactoside in melanoma recurrence.Eur J Dermatol 1995; 5:600–606.
Consdens R, Donble A, Glynn LE, Nind AP. Production of a chronic arthritis with ovalbumin. Its retention in rabbit knee joint.Ann Rheum Dis 1971; 30: 307–315.
Jasin HE. Chronic arthritis in rabbits. In: Di Sabato G, ed.Methods in enzymology, volume 162. Immunochemical techniques. Part L: Chemotaxis and inflammation. San Diego: Academic Press; 1988: 379–385.
De Schrijver M, Streule K, Senekowitsch R, Fridrich R. Scintigraphy of inflammation with nanometer-sized colloidal tracers.Nucl Med Commun 1987; 8: 895–908.
Pittet JC, Pepin M, Normier G, Binz H, Le Pape A. Lymphoscintigraphy via the targeting of macrophates with99mTc-J001X poly-galactoside in a model of pyogranulomas developed in sheep lymph nodes.Nucl Med Biol 1995; 22: 355–365.
Yanni G, Whelan A, Feighery C, Bresnihan B. Greater monocyte/macrophage numbers in rheumatoid synovial membrane predict a worse radiological outcome.Arthritis Rheum 1991; 34: S 117.
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Miot-Noirault, E., Perin, F., Routledge, L. et al. Macrophage targeting with technetium-99m labelled J001 acylated poly-galactoside for scintigraphy of inflammation: Optimization and assessment of imaging specificity in experimental arthritis. Eur J Nucl Med 23, 61–68 (1996). https://doi.org/10.1007/BF01736991
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DOI: https://doi.org/10.1007/BF01736991