Skip to main content
SpringerLink
Log in

Integration and regulation of mitochondrial assembly in yeast

  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Summary

The interactions between the mitochondrial and nucleocytoplasmic systems required for mitochondriogenesis have been investigated at several different levels. Those involved in the formation of functional enzyme complexes have been studied using cytochrome oxidase: this multimeric (2 × 7 and 2 × 6 subunits for enzymes from yeast and beef heart respectively) has been resolved, and the mitochondrial contribution has been shown to be dispensible for catalytic function proper. Using novel mutants, with a mitochondrial mode of inheritance, a mitochondrial gene product localized in the oligomycin-sensitive ATPase has been implicated in the assembly not only of this complex, but of cytochrome oxidase as well. Interactions required for the genetic competence of the mitochondrial system have become apparent as a result of studies in the mechanism of action of the highly effective mitochondrial mutagen ethidium bromide. This agent first becomes covalently inserted into mitochondrial DNA and, after its excision, eventually results in extensive degradation of the macromolecule. The excision reaction has now been shown to be performed by a complex between the oligomycin-sensitive ATPase and a DNA-binding protein presumably involved in recognizing the damage. On the level of replication and expression of the mitochondrial genome studies using thermolabile mutants have demonstrated that these processes appear independent of the replication of nuclear DNA but not of its expression.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Ashwell, M. and Work, T. S., 1970. Ann. Rev. Biochem. 39, 251–290.

    Google Scholar 

  2. Borst, P., 1972. Ann. Rev. Biochem. 41, 333–376.

    Google Scholar 

  3. Sager, R., 1972. Cytoplasmic Genes and Organelles. Academic Press, New York.

    Google Scholar 

  4. Linnane, A. W., Haslem, J. M., Lukins, H. B. and Nagley, P., 1972. Ann. Rev. Microbiol. 26, 163–198.

    Google Scholar 

  5. Mahler, H. R., 1973. CRC Crit. Rev. Biochem. 1, 381–460.

    Google Scholar 

  6. Kroon, A. D. and Saccone, C., 1974. eds., The Biogenesis of Mitochondria. Academic Press, New York.

    Google Scholar 

  7. Schatz, G. and Mason, T., 1974. Ann. Rev. Biochem. 43, 51–87.

    Google Scholar 

  8. Mahler, H. R., Bastos, R. N., Flury, U., Lin, C. C. and Phan, S. H., 1975. In Genetics and Biogenesis of Mitochondria and Chloroplasts (P. S. Perlman, C. W. Birky, Jr. and T. J. Byers, eds.) Ohio State University Press, Columbus, pp. 66–116.

    Google Scholar 

  9. Attardi, G., Constantino, P., England, J., Lynch, D., Murphy, W., Ojala, D., Posakony, J. and Storrie, B., 1975. In Genetics and Biogenesis of Mitochondria and Chloroplasts (P. S. Perlman, C. W. Birky, Jr. and T. J. Byers, eds.) Ohio State University Press, Columbus, 3–65; see also Constantino, P. and Attardi, G., 1975. J. Mol. Biol. 96, 291–306.

    Google Scholar 

  10. Perlman, P. S. and Mahler, H. R., 1975. J. Bioenergetics 1, 113–138.

    Google Scholar 

  11. Perlman, P. S. and Mahler, H. R., 1974. Arch. Biochem. Biophys. 162, 248–271.

    Google Scholar 

  12. Weislogel, P. O. and Butow, R. A., 1971. J. Biol. Chem. 246, 5113–5119.

    Google Scholar 

  13. Ibrahim, N. G., Stuchell, R. N. and Beattie, D. S., 1973. Eur. J. Biochem. 36, 519–527.

    Google Scholar 

  14. Rogers, P. J., Yue, S. B. and Stewart, P. R., 1974. J. Bact. 118, 523–533.

    Google Scholar 

  15. Mahler, H. R. and Raff, R. A., 1976. Intl. Rev. Cytol., 43, 1–124.

    Google Scholar 

  16. Hawley, E. S. and Greenawalt, J. W., 1975. Eur. J. Biochem. 54, 585–601.

    Google Scholar 

  17. Tzagoloff, A., Rubin, M. S. and Sierra, M. F., 1975. Biochim. Biophys. Acta 301, 71–104.

    Google Scholar 

  18. Tzagoloff, A., Akai, A. and Rubin, M. S., 1974. In The Biogenesis of Mitochondria (A. D. Kroon and C. Saccone, eds.) pp. 405–421, Academic Press, New York.

    Google Scholar 

  19. Sebald, W., Machleidt, W. and Otto, J., 1973. Eur. J. Biochem. 38, 311–324.

    Google Scholar 

  20. Weiss, H. and Ziganke, B., 1974. Eur. J. Biochem. 41, 63–71.

    Google Scholar 

  21. Kuboyama, M., Yong, F. C. and King, T. E., 1972. J. Biol. Chem. 247, 6375–6383.

    Google Scholar 

  22. Shakespeare, P. and Mahler, H. R., 1971. J. Biol. Chem. 246, 7649–7655.

    Google Scholar 

  23. Rubin, M. S. and Tzagoloff, A., 1973. J. Biol. Chem. 248, 4269–4274.

    Google Scholar 

  24. Mason, T. L., Poyton, R. O., Wharton, D. C. and Schatz, G., 1973. J. Biol. Chem. 248, 1346–1354.

    Google Scholar 

  25. Poyton, R. O. and Schatz, G., 1975. J. Biol. Chem. 250, 752–761; 762–766.

    Google Scholar 

  26. Phan, S. H. and Mahler, H. R., 1976. J. Biol. Chem., 251, 257–269; 270–276.

    Google Scholar 

  27. Ebner, E., Mason, T. L. and Schatz, G., 1973. J. Biol. Chem. 248, 5269–5278.

    Google Scholar 

  28. Harmon, H. J., Hall, J. D. and Crane, F. L., 1974. Biochim. Biophys. Acta 344, 119–155.

    Google Scholar 

  29. Ebner, E., Mennucci, L. and Schatz, G., 1973. J. Biol. Chem. 248, 5260–5268.

    Google Scholar 

  30. Rose, E., Ebner, E., Poyton, R. O., Mason, T. L., Ono, B. and Schatz, G., 1974. In The Biogenesis of Mitochondria (A. D. Kroon and C. Saccone, eds.) pp. 477–489, Academic Press, New York.

    Google Scholar 

  31. Goffeau, A., Landry, Y., Foury, F., Briquet, M. and Colsin, A-M., 1973. J. Biol. Chem. 248, 7097–7105; see also Goffeau, A., 1975. In Membrane Biogenesis: Mitochondria, Chloroplasts and Bacteria (A. A. Tzagoloff, ed.) pp. 63–97, Plenum Press, New York.

    Google Scholar 

  32. Flury, U., Mahler, H. R. and Feldman, F., 1974. J. Biol. Chem. 249, 6130–6137.

    Google Scholar 

  33. Bastos, R., 1975. J. Biol. Chem., 250, 7739–7746.

    Google Scholar 

  34. Knowles, A. F., 1971. Accts. Chem. Res. 5, 155–160.

    Google Scholar 

  35. Tzagoloff, A. and Meagher, P. 1972. J. Biol. Chem. 247, 594–603; Sierra, M. F. and Tzagoloff, A., 1973. Proc. Nat. Acad. Sci. USA 70, 3155–3159.

    Google Scholar 

  36. Tzagoloff, A. and Akai, A., 1972. J. Biol. Chem. 247, 6516–6523.

    Google Scholar 

  37. Michel, R. and Neupert, W., 1973. Eur. J. Biochem. 36, 52–67.

    Google Scholar 

  38. Werner, S., 1974. Eur. J. Biochem. 43, 39–48; Klein, J. L., Edwards, D. I. and Werner, S., 1975. J. Biol. Chem. 250, 5852–5858.

    Google Scholar 

  39. Küntzel, H. and Blossey, H.-C., 1974. Eur. J. Biochem. 47, 165–171; Küntzel, H., Pieniazek, N. J., Pieniazek, D. and Leister, D. E., 1975. Eur. J. Biochem. 54, 567–575.

    Google Scholar 

  40. Wheeldon, L. W., Dianoux, A.-C., Bof, M. and Vignais, P. V., 1974. Eur. J. Biochem. 46, 189–199.

    Google Scholar 

  41. Hatefi, Y., Stigall, D. L., Galante, Y. and Hanstein, W. C., 1974. Biochem. Biophys. Res. Commun. 61, 313–321.

    Google Scholar 

  42. Slonimski, P. P., Perrodin, G. and Croft, J. H., 1968. Biochem. Biophys. Res. Commun. 30, 232–239.

    Google Scholar 

  43. Perlman, P. S. and Mahler, H. R., 1971. Nature New Biol. 231, 12–16.

    Google Scholar 

  44. Mahler, H. R., 1973. J. Supramol. Struct. 1, 449–460.

    Google Scholar 

  45. Mahler, H. R., 1973. In Molecular Cytogenetics (B. Hamkalo and J. Papaconstantinou, eds.) pp. 181–209, Plenum Press, New York.

    Google Scholar 

  46. Mahler, H. R. and Bastos, R. N., 1974. FEBS Lett. 39, 27–34.

    Google Scholar 

  47. Mahler, H. R. and Bastos, R. N., 1974. Proc. Nat. Acad. Sci. USA 71, 2241–2245.

    Google Scholar 

  48. Bastos, R. N. and Mahler, H. R., 1974. J. Biol. Chem. 249, 6617–6627.

    Google Scholar 

  49. Shannon, C., Enns, R., Wheelis, L., Burchiel, K. and Criddle, R. S., 1973. J. Biol. Chem. 248, 3004–3011.

    Google Scholar 

  50. Griffiths, E. D. and Houghton, R. L., 1974. Eur. J. Biochem. 46, 157–167.

    Google Scholar 

  51. Durphy, M., Manley, P. N. and Friedberg, E. C., 1974. J. Cell Biol. 62, 695–706.

    Google Scholar 

  52. Sena, E. P., Welch, J. W., Halvorson, H. O. and Fogel, S., 1975. J. Bact. 123, 497–504.

    Google Scholar 

  53. Williamson, D. H. and Moustacchi, E., 1971. Biochem. Biophys. Res. Commun. 42, 195–201.

    Google Scholar 

  54. Grimes, G. W., Mahler, H. R. and Perlman, P. S., 1974. J. Cell Biol. 61, 565–574.

    Google Scholar 

  55. Hartwell, L. H., 1973. J. Bact. 115, 966–974.

    Google Scholar 

  56. Hartwell, L. H., Culotti, J., Pringle, J. R. and Reid, B. J., 1974. Science 183, 46–51.

    Google Scholar 

  57. Mahler, H. R., Assimos, K. and Lin, C. C., 1975. J. Bact. 123, 637–641.

    Google Scholar 

  58. Stolarsky, L., Walfield, A. M., Birch, R. A. and Hershberger, C. L., 1976. Biochim. Biophys. Acta, 425, 438–450.

    Google Scholar 

  59. Hartwell, L. H. and McLaughlin, C. S., 1968. Proc. Nat. Acad. Sci. USA 59, 422–428.

    Google Scholar 

  60. Shiokawa, K. and Pogo, A. O., 1974. Proc. Nat. Acad. Sci. USA 71, 2658–2662.

    Google Scholar 

  61. Mahler, H. R. and Dawidowicz, K., 1973. Proc. Nat. Acad. Sci. USA 70, 111–114.

    Google Scholar 

  62. Feldman, F. and Mahler, H. R., 1974. J. Biol. Chem. 249, 3702–3709.

    Google Scholar 

  63. Beattie, D. S., Lin, L.-F. H. and Stuchell, R. N., 1974. In The Biogenesis of Mitochondria (A. D. Kroon and C. Saccone, eds.) pp. 465–476, Academic Press, New York.

    Google Scholar 

Download references

Author information

Author notes

  1. Roberto de N. Bastos

    Present address: Department of Biochemistry, Weizmann Institute of Science, Rehovot, Israel

Authors and Affiliations

  1. Mitochondrial Biogenesis Group, Chemical Laboratories, Indiana University, 47401, Bloomington, IN, USA

    Henry R. Mahler, Sem H. Phan & Roberto de N. Bastos

Authors
  1. Henry R. Mahler
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sem H. Phan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Roberto de N. Bastos
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Recipient of Research Career Award K06 5060 from the National Institute of General Medical Sciences, National Institutes of Health; research supported by Research Grant GM 12228 from this Institute.

Publication No. 2251.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mahler, H.R., Phan, S.H. & Bastos, R.d.N. Integration and regulation of mitochondrial assembly in yeast. Mol Cell Biochem 14, 67–79 (1977). https://doi.org/10.1007/BF01734167

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01734167

Keywords

Search

Navigation