Molecular and Cellular Biochemistry

, Volume 14, Issue 1–3, pp 37–45 | Cite as

The character of protein-nucleic interaction in relation to the mtDNA-membrane complex

  • T. B. Kazakova
  • M. P. Mel'Nikova
  • E. D. Sverdlov


Specific sites that interact with structural proteins of the mitochondrial inner membrane were found in mitochondrial DNA (mtDNA) of rat liver. Analysis of the isolated DNA fragments revealed their capacity to form a complex with membrane proteinsin vitro and allowed the detection of a protein with a molecular weight 40,000. The size of the fragments was found to be 12–18 nucleotide pairs with an average molecular weight 10,000 MtDNA sites recognized by membrane protein proved to be quite unique in having a secondary structure, a high content of AT sequences (82%) and oligopyrimidine blocks. It was shown that the light mtDNA strand, rich in adenine, is 60% more active in the binding with membrane mitochondria than the heavy one.


Molecular Weight Nucleotide Secondary Structure Membrane Protein Adenine 
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Copyright information

© Dr. W. Junk b.v. Publishers 1977

Authors and Affiliations

  • T. B. Kazakova
    • 1
    • 2
  • M. P. Mel'Nikova
    • 1
    • 2
  • E. D. Sverdlov
    • 1
    • 2
  1. 1.Laboratory of Biochemical Genetics, Institute of Experimental MedicineAcademy of Medical SciencesLeningradUSSR
  2. 2.Laboratory of Nucleic Acids Chemistry, Institute of Bioorganic ChemistryAcademy of SciencesMoscowUSSR

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