Skip to main content
SpringerLink
Log in

Genetic variability of soluble proteins studied by two-dimensional electrophoresis on different inbred mouse strains and on different mouse organs

  • Published:
Journal of Molecular Evolution Aims and scope Submit manuscript

Summary

The soluble proteins of four organs (liver, kidney, brain and muscle) of mice from four inbred strains (C57BL/6J, DBA/2J, AKR/J and BALB/cHan) and offspring from cross-breedings therefrom are investigated for genetic variants. The female mice from each strain are divided into different groups according to age (12–14 and 24–26 weeks) and generation (P and F1). The proteins are separated by two-dimensional gel electrophoresis (2DE) using two different techniques (2D GE and 2D SDS-GE), developed in our laboratory.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Anderson NL, Anderson NG (1979) Microheterogeneity of serum transferrin, Haptoglobin andα 2HS glycoprotein examined by high resolution two-dimensional electrophoresis. Biochem Biophys Res Commun 88:258–265

    Google Scholar 

  • Barker WC, Dayhoff MO (1979) Evolution of homologous physiological mechanisms based on protein sequence data. Comp Biochem Physiol 62B:1–5

    Google Scholar 

  • Britten RJ, Davidson EH (1976) DNA sequence arrangement and preliminary evidence on its evolution. Fed Proc 35:2152–2157

    Google Scholar 

  • Brown AJL, Langley CH (1979) Reevaluation of level of genic heterozygosity in natural population of Drosophilia melano-gaster by two-dimensional electrophoresis. Proc Natl Acad Sci USA 76:2381–2384

    Google Scholar 

  • Bucknall WE, Kazazian HH Jr, Clement LT, Childs B (1975) Electrophoretic study of human ribosomal core proteins: Evidence for selection against variation. Johns Hopkins Med J 137:123–126

    Google Scholar 

  • Clarke B, Allendorf FW (1979) Frequency-dependent selection due to kinetic differences between allozymes. Nature 279: 732–734

    Google Scholar 

  • Davis BJ (1964) Disc electrophoresis — II. Method and application to human serum proteins. Ann NY Acad Sci 121:404–427

    Google Scholar 

  • de Jong WW, Zweers A, Cohen LH (1978) Influence of single amino acid substitutions on electrophoretic mobility of sodium dodecyl sulfate-protein complexes. Biochem Biophys Res Commun 82:532–539

    Google Scholar 

  • Elliott RW (1979) Use of two-dimensional electrophoresis to identify and map new mouse genes. Genetics 91:295–308

    Google Scholar 

  • Ephrussi B (ed) (1972) Hybridization of somatic cells. Princeton, New Jersey, Princeton University Press

    Google Scholar 

  • Ferris SD, Whitt GS (1979) Evolution of the differential regulation of duplicate genes after polyploidization. J Mol Evol 12:267–317

    Google Scholar 

  • Fisher SE, Whitt GS (1978) Evolution of isozyme loci and their differential tissue expression. J Mol Evol 12:25–55

    Google Scholar 

  • Gillespie JH, Kojima KI (1968) The degree of polymorphism in enzymes involved in energy production compared to that in nonspecific enzymes in two drsophilia ananassae populations. Proc Natl Acad Sci USA 61:582–585

    Google Scholar 

  • Harris H (1971) Protein polymorphism in man. Can J Genet Cytol 13:381–396

    Google Scholar 

  • Holtzer H, Abbott J (1968) Oscillation of the chondrogenic phenotype in vitro. Cell Differ 1:1–16

    Google Scholar 

  • Johnson GB (1974) Enzyme polymorphism and metabolism. Science 184:28–37

    Google Scholar 

  • King JL (1973) The probability of electrophoretic identity of proteins as a function of amino acid divergence. J Mol Evol 2:317–322

    Google Scholar 

  • King JL (1976) Progress in the neutral mutation — random drift controversy. Fed Proc 35:2087–2091

    Google Scholar 

  • Klose J (1975 a) Protein mapping by combined isoelectric focusing and electrophoresis of mouse tissue. A novel approach to testing for induced point mutations in mammals. Humangenetic 26:231–243

    Google Scholar 

  • Klose J (1975 b) Protein mapping as a tool for investigating mutagenic and teratogenic effects in mouse embryos. In: Neubert D, Merker HJ (eds) New approaches to the evaluation of abnormal embryonic development. Thieme, Stuttgart, pp 375–387

    Google Scholar 

  • Klose J (1979) Isoelectric focusing and electrophoresis combined as a method for defining new point mutations in the mouse. Genetics 92:S13–24 supplement: Proceedings of the workshop: Methods in mammalian mutagenesis, Bar Harbor (Maine), 1976

    Google Scholar 

  • Klose J, Blohm J, Gerner I (1977) The use of isoelectric focusing and electrophoresis to obtain highly complex protein patterns of mouse embryos. In: Neubert D, Merker HJ, Kwasigroch TE (eds) Methods in Prenatal Toxicology. Thieme, Stuttgart, pp 303–313

    Google Scholar 

  • Klose J, Feller M (1981 a) Two-dimensional electrophoresis of membrane and cytosol proteins of mouse liver and brain. Electrophoresis 2:12–24

    Google Scholar 

  • Klose J, Feller M (1981 b) Genetic variability of proteins from plasma membranes and cytosols of mouse organs. Biochem Genet 19:859–870

    Google Scholar 

  • Klose J, Nowak J, Kade W (1980) Two-dimensional electro-phorsis: high resolution of proteins and automatic evaluation of the patterns under different methodical conditions. In: Radola BJ (ed) Electrophoresis '79, Walter de Gruyter, Berlin New York, pp 297–312

    Google Scholar 

  • Klose J, von Wallenberg-Pachaly H (1976) Changes of soluble protein populations during organogenesis of mouse embryos as revealed by protein mapping. Dev Biol 51:324–331

    Google Scholar 

  • Koehn RK, Eanes WF (1977) Subunit size and genetic variation of enzymes in natural populations of drosophilia. Theor Pop Biol 11:330–341

    Google Scholar 

  • Lee CY, Charles D, Bronson D, Griffin M, Bennet L (1979) Analysis of mouse and drosophila proteins by 2-dimensional gel electrophoresis. Mol Gen Genet 176:303–312

    Google Scholar 

  • Macko V, Stegemann H (1969) Mapping of potato proteins by combined electrofocusing and electrophoresis. Identification of varietes. Hoppe-Seylers Z Physiol Chem 350:917–919

    Google Scholar 

  • Markert CL, Shaklee JB, Whitt GS (1975) Evolution of a gene. Science 189:102–114

    Google Scholar 

  • McConkey EH, Taylor BJ, Phan D (1979) Human heterozygosity: A new estimate. Proc Natl Acad Sci USA 76:6500–6504

    Google Scholar 

  • Nei M, Chakraborty R (1973) Genetic distance and electrophoretic identiy of proteins between taxa. J Mol Evol 2: 323–328

    Google Scholar 

  • Nei M, Fuerst PA, Chakraborty R (1978) Subunit molecular weight and genetic variability of proteins in natural populations. Proc Natl Acad Sci USA 75:3359–3362

    Google Scholar 

  • O'Farrell PH (1975) High resolution two-dimensional electrophoresis of proteins. J Biol Chem 250:4007–4021

    Google Scholar 

  • Paigen K (1979) Acid hydrolases as models of genetic control. Ann Rev Genet 13:417–466

    Google Scholar 

  • Potter M (1978) Comments on the relationship of inbred strains to the genus mus. In: Morse HC (ed) Origins of inbred mice. Academic Press, New York San Francisco London, pp 497–509

    Google Scholar 

  • Racine RR, Langley CH (1980 a) Genetic heterozygosity in a natural population of Mus musculus assessed using two-dimensional electrophoresis. Nature 283:855–857

    Google Scholar 

  • Racine RR, Langley CH (1980 b) Genetic analysis of protein variations in Mus musculus using two-dimensional electrophoresis. Biochem Genet 18:185–198

    Google Scholar 

  • Roberts DB (1975) Drosophila antigens: Their spatial and temporal distribution, their function and control. Curr Top Dev Biol 9:167–189

    Google Scholar 

  • Roderick TH, Ruddle FH, Chapman VM, Shows TB (1971) Biochemical polymorphisms in feral and inbred mice (Mus musculus). Biochem Genet 5:457–466

    Google Scholar 

  • Sarich VM (1977) Rates, sample sizes, and the neutrality hypothesis for electrophoresis in evolutionary studies. Nature 265:24–28

    Google Scholar 

  • Taylor BA (1972) Genetic relationships between inbred strains of mice. J Heredity 63:83–86

    Google Scholar 

  • Walton KE, Styer D, Gruenstein EI (1979) Genetic polymorphism in normal human fibroblasts as analyzed by two-dimensional polyacrylamide gel electrophoresis. J Biol Chem 254:7951–7960

    Google Scholar 

  • Ward RD (1978) Subunit size of enzymes and genetic heterozygosity in vertebrates. Biochem Genet 16:799–810

    Google Scholar 

  • Wilson AC, Carlson SS, White TJ (1977) Biochemical evolution. Ann Rev Biochem 46:573–639

    Google Scholar 

  • Wilson AC, Maxson LR, Sarich VM (1974) Two types of molecular evolution, evidence from studies of interspecific hybridization. Proc Natl Acad Sci USA 71:2843–2847

    Google Scholar 

  • Zeindl E, Sperling K, Klose J (1982) Mutagenicity testing on non-selectively cloned chinese hamster ovary cells using the protein mapping method. Mutat Res 97:67–78

    Google Scholar 

  • Zuckerkandl E (1963) Perspectives in moelcular anthorpology. In: Washburn SL (ed) Classification and human evolution. Aldine Publishing Co, Chicago, p 243

    Google Scholar 

  • Zuckerkandl E (1964) Controller-gene diseases: The operon model as applied toβ-Thalassemia, familial fetal hemoglo-binemia and the normal switch from the production of fetal hemoglobin to that of adult hemoglobin. J Mol Biol 8:128–147

    Google Scholar 

  • Zuckerkandl E (1975) The appearance of new structures and functions in proteins during evolution. J Mol Evol 7:1–57

    Google Scholar 

  • Zuckerkandl E (1976) Evolutionary processes and evolutionary noise at the molecular level. J Mol Evol 7:167–183

    Google Scholar 

  • Zuckerkandl E (1978) Multilocus enzymes, gene regulation, and genetic sufficiency. J Mol Evol 12:57–89

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Humangenetik, Institut für Toxikologie und Embryonalpharmakologie, Abt. Genetik, Freie Universität Berlin, Garystraße 1-9, 1000, Berlin 33, Federal Republic of Germany

    J. Klose

Authors
  1. J. Klose
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Klose, J. Genetic variability of soluble proteins studied by two-dimensional electrophoresis on different inbred mouse strains and on different mouse organs. J Mol Evol 18, 315–328 (1982). https://doi.org/10.1007/BF01733898

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01733898

Keywords

Search

Navigation