Summary
A series of five alternating poly(leucyl-lysyl) samples with varying amounts of L- and D-residues randomly distributed along the chain, but evenly shared out amongst leucyl and lysyl residues were synthesized by condensation of a mixture of the four diastereoisomeric dipeptidep-nitro-phenylesters. Their behavior in aqueous solution at various ionic strengths was studied by infrared spectroscopy which allowed measurement of the total amount ofβ-structures, and by circular dichroism which gives the excess of L-residues over D-residues in the same structures. Comparison with the properties of the all L-poly(Lys-Leu-Lys-Leu) shows that incorporation of a few D-residues in a L-chain seems to reduce the width of theβ-sheets obtained in presence of salt. Higher proportions of D-isomers prevent the coil →β transition from occurring when the ionic strength is increased except for segments containing at least 6 to 7 adjacent residues of the same configuration.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Akaike, T., Inoue, S., Itoh, K. (1976). Biopolymers15, 1863–1868
Ballard, D.G.H., Bamford, C.H., Elliott, A. (1960). Makromol. Chem.35, 222–238
Benoit, H., Freund, L., Spach, G. (1967). In: Polyα-Amino Acids, G.D. Fasman, ed., p. 105, New York: M. Dekker
Bonora, G.N., Toniolo, C. (1974). Biopolymers13, 2179–2190 and references therein
Brack, A. (1977). Biosystems9, 99–103
Brack, A., Caille, A. (1978). Int. J. Peptide Protein Res.11, 128–139
Brack, A., Orgel, L.E. (1975). Nature (Lond.)256, 383–387
Colonna-Cesari, F., Premilat, S., Heitz, F., Spach, G., Lotz, B. (1977). Macromolecules10, 1284–1288
Heitz, F., Lotz, B., Spach, G. (1975). J. Mol. Biol.92, 1–23
Hesselink, F.T., Scheraga, H.A. (1972). Macromolecules5, 455–463
Le Guilly, L., Brack, A., Spach, G. (1978). Makromol. Chem.179, 2829–2835
Lotz, B. (1974). J. Mol. Biol.87, 169–180
Lotz, B., Colonna-Cesari, F., Heitz, F., Spach, G. (1976). J. Mol. Biol.106, 915–942
Lotz, B., Heitz, F., Spach, G. (1973). C.R. Acad. Sci. Paris276C, 1715–1718
Miller, W.G., Brant, D.A., Flory, P.J. (1967). J. Mol. Biol.23, 67–80
Pauling, L., Corey, R.B. (1951). Proc. Natl. Acad. Sci. U.S.37, 251–256
Pauling, L., Corey, R.B. (1953). Proc. Natl. Acad. Sci. U.S.39, 253–256
Ramachandran, G.N., Chandrasekaran, R. (1972). Ind. J. Biochem. Biophys.9, 1–11
Rosenheck, K., Doty, P. (1961). Proc. Natl. Acad. Sci. U.S.47, 1775–1785
Spach, G. (1969). C.R. Acad. Sci. Paris249, 543–546
Spach, G., Brack, A., Heitz, F. (1968). C.R. Acad. Sci. Paris267C, 809–812
Veatch, W.R., Blout, E.R. (1974). Biochemistry13, 5257–5264
Woody, R.W. (1969). Biopolymers8, 669–683
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Brack, A., Spach, G. β-structures of polypeptides with L- and D-residues. J Mol Evol 13, 35–46 (1979). https://doi.org/10.1007/BF01732752
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01732752