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Aminoacyl-tRNA synthetases from calf liver: Optimized assay conditions and kinetic properties

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Summary

Kinetic studies have been performed on the “family” of aminoacyl synthetases from calf liver. All assays were based on the esterification of amino acids to tRNA. Optimized reaction conditions for each synthetase are reported. Most of the synthetases show hyperbolic kinetics with respect to both amino acid and tRNA concentration, however a few show sigmoidal kinetics with respect to one substrate. Arginine, methionine and proline synthetases show sigmoidal kinetics with respect to mixed tRNA solutions and have Hill coefficients of 1.30, 1.10 and 1.20 respectively. Alanine and isoleucine synthetases show sigmoidal kinetics with respect to amino acid concentration and have Hill coefficients of 1.21 and 1.40 respectively.

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References

  1. Hancher, C. W., Pearson, R. L. and Kelmers, A. D. 1974. Methods in Enzymology, Vol. XXIX, Part E. 510–514.

    Google Scholar 

  2. Robison, B. and Zimmerman, T. P. 1970. Anal. Biochem. 37, 11–20.

    Google Scholar 

  3. Rogg, H., wehrli, W. and Staehelin, M. 1969. Biochem. Biophys. Acta. 195, 13–15.

    Google Scholar 

  4. Singer, M. F. and Tolbert, G. 1965. Biochemistry 4, 1319–1330.

    Google Scholar 

  5. Birnboim, H. C. 1970. Anal. Biochem. 37, 178–182.

    Google Scholar 

  6. Mehler, A. H. 1971.In Methods in Enzymology, Vol. XX, Part C., ed. L. Grossman and K. Moldave. Academic Press, N.Y. pp. 203–210.

    Google Scholar 

  7. Yang, W. K. and Novelli, G. D. 1968. Proc. Nat. Acad. Sci. U.S.A. 59, 208–215.

    Google Scholar 

  8. Muench, K. H. and Berg, P. 1966.In: Procedures in Nucleic Acid Research, eds. G. L. Cantoni and D. R. Davies. Harper & Row, N. Y., pp. 375–383.

    Google Scholar 

  9. Hill, A. V. 1913. Biochem. J. 7, 471–480.

    Google Scholar 

  10. Francis, T. A. and Nagel, G. M. 1976. Biochem. Biophysics. Res. Comm. 70, 862–868.

    Google Scholar 

  11. Hertz, H. S. and Zachau, H. G. 1973. Eur. J. Biochem. 37, 203–213.

    Google Scholar 

  12. Krauss, G.,, Peters, F. and Maass, G. Eur. J. Biochem. 55, 517–529.

  13. Fersht, A. R. 1975. Biochem. 14 (1), 5–12.

    Google Scholar 

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Authors and Affiliations

  1. Department of Biology, York University, Downsview, Ontario, Canada

    Alfred H. F. Choo & David M. Logan

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  1. Alfred H. F. Choo
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  2. David M. Logan
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Supported by a Grant From the National Research Council of Canada

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Choo, A.H.F., Logan, D.M. Aminoacyl-tRNA synthetases from calf liver: Optimized assay conditions and kinetic properties. Mol Cell Biochem 17, 31–38 (1977). https://doi.org/10.1007/BF01732552

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  • DOI: https://doi.org/10.1007/BF01732552

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