Journal of Molecular Evolution

, Volume 5, Issue 1, pp 35–46 | Cite as

The nucleotide sequence of the 5S ribosomal RNA from a photobacterium

  • Carl R. Woese
  • Cheryl D. Pribula
  • George E. Fox
  • Lawrence B. Zablen


Comparative sequencing studies provide powerful insights into molecular function and evolution. The sequence for 5S ribosomal RNA from Photobacter strain 8265 is eighteen base replacements removed from that ofEscherichia coli. Of these, the vast majority involve a G or C becoming an A or U. These variations also define unequivocally a hexanucleotide base paired region, which appears to be a universal feature of the 5S RNA molecule. The base composition of this helix seems to be under rather stringent, and so unusual, energetic constraints. The possible implications of this are discussed - in particular the prospect of a 5S RNA molecule that undergoes conformational transitions as a part of the overall state changes that constitute the function of the ribosome.

Key words

Photobacter Strain 8265 5S Ribosomal RNA Primary Structure Comparative Characterization Evolution Energetically Constrained Helix 


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Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • Carl R. Woese
    • 1
  • Cheryl D. Pribula
    • 1
  • George E. Fox
    • 1
  • Lawrence B. Zablen
    • 1
  1. 1.Provisional Dept. of Genetics and DevelopmentUniversity of IllinoisUrbanaUSA

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