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Journal of Molecular Evolution

, Volume 5, Issue 1, pp 35–46 | Cite as

The nucleotide sequence of the 5S ribosomal RNA from a photobacterium

  • Carl R. Woese
  • Cheryl D. Pribula
  • George E. Fox
  • Lawrence B. Zablen
Article

Summary

Comparative sequencing studies provide powerful insights into molecular function and evolution. The sequence for 5S ribosomal RNA from Photobacter strain 8265 is eighteen base replacements removed from that ofEscherichia coli. Of these, the vast majority involve a G or C becoming an A or U. These variations also define unequivocally a hexanucleotide base paired region, which appears to be a universal feature of the 5S RNA molecule. The base composition of this helix seems to be under rather stringent, and so unusual, energetic constraints. The possible implications of this are discussed - in particular the prospect of a 5S RNA molecule that undergoes conformational transitions as a part of the overall state changes that constitute the function of the ribosome.

Key words

Photobacter Strain 8265 5S Ribosomal RNA Primary Structure Comparative Characterization Evolution Energetically Constrained Helix 

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References

  1. Aubert, R., Bellemare, G., Monier, R. (1973). Biochim. 55, 135Google Scholar
  2. Bellemare, G., Jordan, B.R., Rocca-Serra, J., Monier, R. (1972). Biochim. 54, 1453Google Scholar
  3. Brownlee, G.G., Cartwright, E., McShane, T., Williamson, R. (1972). FEBS Lett. 25, 8Google Scholar
  4. Brownlee, G.G., Sanger, F., Barrel, B.G. (1967). Nature (Lond.) 215, 735Google Scholar
  5. Dubuy, B., Weissman, S.M. (1971). J.Biol.Chem. 246, 747Google Scholar
  6. Forget, B.G., Weissman, S.M. (1967). Nature (Lond.) 213, 878Google Scholar
  7. Jordan, B.R., Galling, G. (1973). FEBS Lett. 37, 333Google Scholar
  8. Lewis, J.B., Doty, P. (1970). Nature (Lond.) 225, 510Google Scholar
  9. Nishikawa, K., Takemura, S. (1974). FEBS Lett. 40, 106Google Scholar
  10. Ohta, T., Kimura, M. (1971). J.Mol.Evol. 1, 18Google Scholar
  11. Pribula, C.D., Fox, G.E., Woese, C.R. (1974). FEBS Lett. 44, 322Google Scholar
  12. Sanger, F., Brownlee, G.G., Barrell, B.G. (1965). J.Mol.Biol. 13, 373Google Scholar
  13. Sogin, M., Pace, B., Pace, N.R., Woese, C.R. (1971). Nature, New Biol. (Lond.) 232, 48Google Scholar
  14. Tinoco, I. Jr., Borer, P.N., Dengler, B., Levine, M.D., Uhlenbeck, O.C. Crothers, D.M., Gralla, J. (1973). Nature, New Biol. 246, 40Google Scholar
  15. Tinoco, I.Jr., Uhlenbeck, O.C., Levine, M.D. (1971). Nature (Lond.) 230, 362Google Scholar
  16. Uchida, T., Bonen, L., Schaup, H.W., Lewis, B.J., Zablen, L., Woese, C. (1974). J.Mol.Evol. 3, 63Google Scholar
  17. Zablen, L., Bonen, L., Meyer, R., Woese, C. (in press). J.Mol.Evol.Google Scholar

Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • Carl R. Woese
    • 1
  • Cheryl D. Pribula
    • 1
  • George E. Fox
    • 1
  • Lawrence B. Zablen
    • 1
  1. 1.Provisional Dept. of Genetics and DevelopmentUniversity of IllinoisUrbanaUSA

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