Summary
Autoradiography and biochemical investigations showed that [3H]-testosterone where injected intraperitoneally into male white rats was incorporated rapidly into thymus lymphocytes. Thymic cortex contained more silver grains than medulla, and larger lymphocytes were more labelled than medium or small lymphocytes.
Cytosol fraction of thymus cells labelledin vivo with [3H]-testosterone, contained the largest quantity of labelled hormone. A 4S cytosol fraction binds [3H]-testosterone. This could be separated by Sephadex chromatography or by linear sucrose gradient centrifugation. Nuclear extract contained also a small quantity of the labelled hormone.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Abraham, A. D. and Sekeris, C. E., 1971. Biochim. Biophys. Acta 247, 562–569.
Abraham, A. D. and Sekeris, C. E., 1973. Biochim. Biophys. Acta 297, 142–154.
Mainwaring, W. J. P., 1969. J. Endocrin. 44, 323–333.
Mainwaring, W. J. P., 1969. J. Endocrin. 45, 531–541.
Fang, S., Anderson, K. M. and Shutsung, Liao, 1969. J. Biol. Chem. 244, 6584–6595.
Robel, P., Blondeau, J. P. and Baulieu, E. E., 1974. Biochim. Biophys. Acta 373, 1–9.
Hu, A-Li, Loor, R. M. and Wang, T. Y., 1975. Biochem. Biophys. Res, Commun. 65, 1327–1334.
Mulder, E., Peters, M. J., de Vries, J. and Van der Molen, H. J., 1975. Mol Cell. Endocrinol. 2, 171–182.
Hansson, V., McLean, W. S., Smith, A. A., Tindall, D. J., Weddington, S. C., Neyfeh, S. M. and French, F. S., 1974. Steroids 23, 823–832.
Ritzen, E. M. and French, F. S., 1974. J. Steriod. Biochem. 5, 151–155.
Tindall, D. J., Hansson, V., Sar, M., Stumph, W. E., French, F. S. and Nayfeh, S. M., 1974. Endocrinology 95, 1119–1129.
Bullock, L. P. and Bardin, C. W., 1974. Endocrinology, 94, 746–757.
Bullock, L. P., Mainwaring, W. J. P., and Bardin, C. W., 1975. Endocrin. Res. Commun. 2, 25.
Samperez, S., Thieulant, M. L., Mercier, L. and Jouan, P., 1974. J. Steroid. Biochem. 5, 911–915.
Valladares, L., and Minguell, J., 1955. Steroids 25, 13–23.
Gustafsson, J. Å., Pousette, Å. Stenberg, Å. and Wrange, O. 1975. Biochemistry 14, 3942–3949.
Michel, G. and Baulieu, E. E., 1974. C. R. S. France, ser. D 279, 421–424.
Kochakian, C. D. and Harrison, D. G., 1962 Endocrinology 1, 99–108.
Van der Meulen, M., Abraham, A. D. and Sekeris, C. E., 1972. FEBS Letters 25, 116–122.
Bell, Ph. and Munck, A., 1973. Biochem. J. 136, 97–107.
Schaumburg, B. P., 1972. Biochim. Biophys. Acta, 261, 211–235; 263, 414–423.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Abraham, A.D., Buga, G. ‘3H-testosterone distribution and binding in RAT thymus cells in vivo’. Mol Cell Biochem 13, 157–163 (1976). https://doi.org/10.1007/BF01731778
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01731778