Molecular and Cellular Biochemistry

, Volume 13, Issue 3, pp 141–146 | Cite as

Hormonal control of FAT cells adenylate cyclase

  • Robert Harris
  • Alfred Bennun
General and Review Articles a. general articles


Fat cells were preincubated for 2 h in the presence and absence of growth hormone (GH) and Dexamethasone (Dex) before the addition of increasing concentrations of either epinephrine, theophylline or glucagon and final incubation of the cells for an additional 5 minutes. GH and Dex increased by 85%, 28% and 72%, respectively, the cAMP levels reached in the sole presence of 10−5m epinephrine, 10−2m theophylline or 5 × 10−5m glucagon. An adenylate cyclase particulate preparation shows that epinephrine decreases Km from 2mm to 0.6mm and increases Vmax and the strength of interaction value (n) from 0.91 to 1.75.


Growth Hormone Dexamethasone Epinephrine Theophylline Glucagon 
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Copyright information

© Dr. W. Junk b.v. Publishers 1976

Authors and Affiliations

  • Robert Harris
    • 1
  • Alfred Bennun
    • 1
  1. 1.Cooperative Graduate Program of Biochemistry RutgersThe State University NewarkUSA

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