Summary
The proteolytic activity of the extracellular protease ofSerratia marcescens was compared with that of trypsin on N, N-dimethyl casein. The peptides produced from exhaustive hydrolysis of alpha casein by the protease and by trypsin were of similar size as measured by gel filtration on P-10 Agarose. We conclude that the protease ofS. marcescens is an endopeptidase with trypsin-like activity on proteins, producing oligopeptides.
End group analysis of the peptides formed by theS. marcescens protease suggests that the protease has a unique substrate specificity, hydrolyzing only a peptide bond whose carboxyl group is donated by proline. The protease was inactive on the synthetic peptides with proline donating the carboxyl group, but hydrolyzed various types of natural proteins. Its narrow and novel substrate specificity makes this enzyme a potential tool for the determination of the primary structure of proteins.
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Aiyappa, P.S., Harris, J.O. The extracellular metalloprotease of serratia marcescens: 2. Comparison with trypsin and substrate specificity. Mol Cell Biochem 13, 131–136 (1976). https://doi.org/10.1007/BF01731774
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DOI: https://doi.org/10.1007/BF01731774