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Insoluble collagen of methylcholanthrene induced sarcoma

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Summary

The insoluble collagen from methylcholanthrene induced sarcoma was isolated and characterized. It contains more glycine, hydroxyproline and acidic amino acids than normal connective tissue collagen. An anionic character of tumour collagen was stated (pI 6.1). No typical collagen subunits in this protein were found.

The tumour collagen is strongly bound to acidic glycoprotein containing a significant amount of hydroxylysine. Such an insoluble complex is resistant to the dispersing action of EDTA. It dissociates during heating in concentrated urea.

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Authors and Affiliations

  1. Department of Biochemistry and Department of General Chemistry, Institute of Physiology and Biochemistry, Academy of Medicine, Bialystok, Poland

    Edward Bańkowski, Andrzej Gindzieński, Elzbieta Pawlicka & Wiktor Rzeczycki

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  1. Edward Bańkowski
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  2. Andrzej Gindzieński
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  3. Elzbieta Pawlicka
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  4. Wiktor Rzeczycki
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Bańkowski, E., Gindzieński, A., Pawlicka, E. et al. Insoluble collagen of methylcholanthrene induced sarcoma. Mol Cell Biochem 8, 23–30 (1975). https://doi.org/10.1007/BF01731646

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  • DOI: https://doi.org/10.1007/BF01731646

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