Summary
Shearing in either a Virtis small or large volume capacity cup under defined conditions gave chromatin fragment size distribution upon agarose exclusion column chromatography (50 × 106) that varied inversely with shear time. The large- and small-sized chromatin fragments possessed high template capacity while the intermediate fragments (the majority) had relatively low template capacity; the differences in template capacity of these size groups varying inversely with the shear time. The protein/DNA and RNA/DNA ratios remained relatively constant in all size groups.
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Duerksen, J.D. Distribution of transcribable DNA sequences in mouse liver hepatoma chromatin. Mol Cell Biochem 4, 197–203 (1974). https://doi.org/10.1007/BF01731481
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DOI: https://doi.org/10.1007/BF01731481