Summary
DL-phenylalanine hydroxamate inhibited the growth ofBacillus subtilis NCTC 8533 and this inhibition was most effectively reversed by L-phenylalanine. Mutants ofB. subtilis, resistant to the analogue, excreted L-phenylalanine into the medium in significant amounts. The accumulation of amino acid was further increased after mutation of analogue-resistant strains to a requirement for tyrosine. Strain THrP-17 was resistant to DL-phenylalanine hydroxamate and had an absolute requirement for tyrosine and accumulated 1.37 g L-phenylalanine per litre of medium.
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McEvoy, J.J., Joyce, A. Production of L-phenylalanine by DL-phenylalanine hydroxamate-resistant tyr− mutants of Bacillus subtilis. Mol Cell Biochem 4, 191–195 (1974). https://doi.org/10.1007/BF01731480
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DOI: https://doi.org/10.1007/BF01731480