The metabolism of acid soluble adenine nucleotides in heat-synchronizedTetrahymena pyriformis GL has been studied. In addition, the effect of the synchronizing temperature (34 °C) on adenine nucleotide metabolism in heat-synchronized cells has been determined. In cells induced to divide synchronously through heat treatment (cyclic pulses of 34 °C for 30 min alternating with a 30 min recovery period at 28 °C) variations occurred in the levels of adenine nucleotides when samples of cells were analysed at the end of the last thermal period and at various times preceding the first synchronous cell division. The specific activities of the adenine nucleotides were found to be significantly higher during a pulse label period performed at the end of the last thermal period than at any time during the subsequent synchronous division cycle. The synchronizing temperature was found to partially deplete the intracellular stores of ATP in heat synchronized cells. This decrease was reversible with ATP levels recovering after 15 min of incubation at 28 °C. Fluctuations in the levels and specific activities of the adenine nucleotides are discussed in their relation to macromolecular synthesis and the cell cycle inTetrahymena.
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Stocco, D.M., Zimmerman, A.M. Adenine nucleotide metabolism in heat-synchronized Tetrahymena. Mol Cell Biochem 7, 187–194 (1975). https://doi.org/10.1007/BF01731408
- Cell Cycle
- Cell Division
- Heat Treatment