Molecular and Cellular Biochemistry

, Volume 7, Issue 3, pp 187–194 | Cite as

Adenine nucleotide metabolism in heat-synchronized Tetrahymena

  • D. M. Stocco
  • A. M. Zimmerman
Review and General Articles b. general articles

Summary

The metabolism of acid soluble adenine nucleotides in heat-synchronizedTetrahymena pyriformis GL has been studied. In addition, the effect of the synchronizing temperature (34 °C) on adenine nucleotide metabolism in heat-synchronized cells has been determined. In cells induced to divide synchronously through heat treatment (cyclic pulses of 34 °C for 30 min alternating with a 30 min recovery period at 28 °C) variations occurred in the levels of adenine nucleotides when samples of cells were analysed at the end of the last thermal period and at various times preceding the first synchronous cell division. The specific activities of the adenine nucleotides were found to be significantly higher during a pulse label period performed at the end of the last thermal period than at any time during the subsequent synchronous division cycle. The synchronizing temperature was found to partially deplete the intracellular stores of ATP in heat synchronized cells. This decrease was reversible with ATP levels recovering after 15 min of incubation at 28 °C. Fluctuations in the levels and specific activities of the adenine nucleotides are discussed in their relation to macromolecular synthesis and the cell cycle inTetrahymena.

Keywords

Nucleotide Cell Cycle Cell Division Adenine Heat Treatment 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Brown, P. R., Anal. Biochem. 43, 305–306 (1971).Google Scholar
  2. 2.
    Burnasheva, S. A. and Kaurasheva, T. P., Biokhimiya 32, 270–276 (1967).Google Scholar
  3. 3.
    Byfield, J. E. and Scherbaum, O. H., J. Cell Physiol. 68, 203–206 (1966).Google Scholar
  4. 4.
    Byfield, J. E. and Scherbaum, O. H., Proc. N.A.S. 57, 602–606 (1967).Google Scholar
  5. 5.
    Byfield, J. E. and Lee, Y. C., J. Protozool. 17, 445–453 (1970).Google Scholar
  6. 6.
    deBarros, A. V., DeCastro, J. F. and DeCastro, F. T., J. Cell. Physiol. 81, 149–152 (1973).Google Scholar
  7. 7.
    Erwin, J. A., Biochim. Biophys. Acat. 202, 21–34 (1970).Google Scholar
  8. 8.
    Jeffery, W. R., Stuart, K. D. and Frankel, J., J. Cell Biol. 46, 533–543 (1970).Google Scholar
  9. 9.
    Kennel, D., Methods in Enzymology (Colowick, S. P. and Kaplan, N. V., eds.) Vol. XII pp. 686–693, Academic Press, New York 1967.Google Scholar
  10. 10.
    Levy, M. R., Gollon, C. E. and Elliott, A. M., Exptl. Cell Res. 55, 295–305 (1969).Google Scholar
  11. 11.
    Moner, J. G., Exptl. Cell Res. 45, 618–630 (1967).Google Scholar
  12. 12.
    Nanney, D. L., J. Exp. Zool. 175, 383–390 (1970).Google Scholar
  13. 13.
    Oikawa, T. G. and Smith, M., Biochem. 5, 1517–1521 (1966).Google Scholar
  14. 14.
    Plesner, P., Compt. Rend. Trav. Lab. Carlsberg 34, 1–76 (1964).Google Scholar
  15. 15.
    Rooney, D. W. and Eiler, J. J., J. Cell Biol. 41, 145–153 (1969).Google Scholar
  16. 16.
    Rosenbaum, N., Erwin, J., Beach, O. and Holz, C. G., J. Protozool. 15, 535–546 (1966).Google Scholar
  17. 17.
    Scherbaum, O. H. and Zeuthen, E., Exptl. Cell Res. 6, 221–227 (1954).Google Scholar
  18. 18.
    Scherbaum, O. H., Chou, S. C., Seraydarian, K. H. and Byfield, J. E., Can. J. Microbiol. 8, 753–760 (1962).Google Scholar
  19. 19.
    Stambrook, P. J. and Sisken, J. E., Biochim. Biophys. Acta 281, 45–54 (1972).Google Scholar
  20. 20.
    Stocco, D. M., Beers, P. C. and Warner, A. H., Develop. Biol. 27, 479–493 (1972).Google Scholar
  21. 21.
    Stocco, D. M. and Zimmerman, A. M., Can. J. Biochem. 52, 310–318 (1974).Google Scholar
  22. 22.
    Warner, A. H. and Finamore, F. J., J. Biol. Chem. 242, 1933–1937 (1967).Google Scholar

Copyright information

© Dr. W. Junk b.v. Publishers 1975

Authors and Affiliations

  • D. M. Stocco
    • 1
  • A. M. Zimmerman
    • 1
  1. 1.Department of ZoologyUniversity of TorontoTorontoCanada

Personalised recommendations