, Volume 99, Issue 3, pp 190–195 | Cite as

The chromosomal distribution of the major and minor satellite is not conserved in the genusMus

  • A. K. C. Wong
  • F. G. Biddle
  • J. B. Rattner


The cytological distribution of the major and minor satellite first identified inMus musculus was studied in the karyotypes of three related subspecies and two other species of the genusMus. Both the major and minor satellite showed species dependent hybridization patterns. The major satellite is confined to the centromere region inM. musculus and related subspecies. However, inM spretus andM. caroli, the chromosomal arm regions contain this sequence class. In contrast the minor satellite is found at the kinetochore region inM. musculus and related subspecies but is distributed throughout the entire centromeric domain inM. spretus and appears to be excluded from the chromosomes ofM. caroli. There is an apparent correlation between the chromosomal location of these satellites and their phylogenetic relationship. Determination of the biological roles of the major and minor satellites fromM. musculus must take into account their differential chromosomal distribution in otherMus species.


Developmental Biology Phylogenetic Relationship Chromosomal Location Biological Role Centromere Region 
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  1. Avila JE, Montejo de Garcini E, Wandosell F, Villasante A, Sogo JM, Villanueva N (1983) Microtubule-associated proteins MAP2 preferentially binds to a dA/dT sequence present in mouse satellite DNA. EMBO J 2:1229–1234Google Scholar
  2. Biddle FG, Nishioka Y (1988) Assays of testis development in the mouse distinguish three classes of domesticus-type Y chromosome. Genome 30:870–878Google Scholar
  3. Bonhomme F (1986) Evolutionary relationships in the genusMus. Curr Top Microbiol Immunol 127:9–34Google Scholar
  4. Brown SDM, Dover GA (1980) Conservation of segmental variants of satellite DNA ofMus musculus in a related species:Mus spretus. Nature 285:47–49Google Scholar
  5. Capanna E, Gropp A, Winking H, Noack G, Civitelli MV (1976) Robertsonian metacentrics in the mouse. Chromosoma 58:341–353Google Scholar
  6. Dod B, Mottez E, Desmarais E, Bonhomme F, Roizes G (1989) Concerted evolution of light satellite DNA in genusMus implies amplification and homogenization of large blocks of repeats. Mol Biol Evol 6:478–491Google Scholar
  7. Goldberg GI, Collier I, Cassel A (1983) Specific DNA sequences associated with the nuclear matrix in synchronized mouse 3T3 cells. Proc Natl Acad Sci USA 80:6887–6891Google Scholar
  8. Horz W, Altenburger W (1981) Nucleotide sequence of mouse satellite DNA. Nucleic Acids Res 9:683–698Google Scholar
  9. Jones KW (1970) Chromosomal and nuclear location of mouse satellite DNA in individual cells. Nature 225:912–915Google Scholar
  10. Lica LM, Narayanswami S, Hamkalo BA (1986) Mouse satellite DNA, centromere structure, and sister chromatid pairing. J Cell Biol 103:1145–1151Google Scholar
  11. Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbour Laboratory, Cold Spring Harbour, NYGoogle Scholar
  12. Marx KA, Denial T, Keller T (1984) High-affinity microtubule protein-higher organism DNA complexes. Many fold enrichment in repetitive mouse DNA sequences comprised of satellite DNAs. Biochim Biophy Acta 783:283–292Google Scholar
  13. Masumoto H, Sugimoto K, Okazaki T (1989) Alphoid satellite DNA is tightly associated with centromere antigens in human chromosomes throughout the cell cycle. Exp Cell Res 181:181–196Google Scholar
  14. Mitchell AR, Gosden JR, Miller DA (1985) A cloned sequence, p82H, of the alphoid repeated DNA family found at the centromeres of all human chromosomes. Chromosoma 92:369–377Google Scholar
  15. Neuer-Nitsche B, Lu X, Werner D (1988) Functional role of a highly repetitive DNA sequence in anchorage of the mouse genome. Nucleic Acids Res 16:8351–8360Google Scholar
  16. Pardue ML, Gall JG (1970) Chromosomal localization of mouse satellite DNA. Science 168:1356–1358Google Scholar
  17. Pietras DF, Bennett KL, Siracusa LD, Woodworth-Gutai M, Chapman VM, Gross KW, Kane-Haas C, Hastie ND (1983) Construction of a smallMus musculus repetitive DNA library: identification of a new satellite sequence inMus musculus. Nucleic Acids Res 11:6965–6983Google Scholar
  18. Pinkel D, Straume T, Gray JW (1986) Cytogenetics analysis using quantitative, high sensitivity, fluorescence hybridization. Proc Natl Acad Sci USA 83:2934–2938Google Scholar
  19. Ponzetto-Zimmerman C, Wolgemuth DJ (1984) Methylation of satellite sequences in mouse spermatogenic and somatic DNAs. Nucleic Acids Res 12:2807–2822Google Scholar
  20. Radic MZ, Lundgren K, Hamkalo BA (1987) Curvature of mouse satellite DNA and condensation of heterochromatin. Cell 50:1101–1108Google Scholar
  21. Rice NR, Straus N (1973) Relatedness of mouse satellite deoxyribonucleic acid to deoxyribonucleic acid of variousMus species. Proc Natl Acad Sci USA 70:3546–3550Google Scholar
  22. Selig S, Ariel M, Goitein R, Marcus M, Cedar H (1988) Regulation of mouse satellite DNA replication time. EMBO J 7:419–426Google Scholar
  23. Southern EM (1975) Long range periodicities in mouse satellite DNA. J Mol Biol 94:51–69Google Scholar
  24. Sutton WD, McCallum M (1972) Related satellite DNAs in the genusMus. J Mol Biol 71:633–656Google Scholar
  25. Wiche G, Corces VG, Avila J (1978) Preferential binding of hog brain microtubule-associated proteins to mouse satellite versus bulk DNA preparations. Nature 273:403–405Google Scholar
  26. Wong AKC, Rattner JB (1988) Sequence organization and cytological localization of the minor satellite of mouse. Nucleic Acids Res 16:11645–11661Google Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • A. K. C. Wong
    • 1
  • F. G. Biddle
    • 1
    • 2
  • J. B. Rattner
    • 1
    • 3
  1. 1.Department of Medical BiochemistryUniversity of CalgaryCalgaryCanada
  2. 2.Department of PediatricsUniversity of CalgaryCalgaryCanada
  3. 3.Department of AnatomyUniversity of CalgaryCalgaryCanada

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