Molecular and Cellular Biochemistry

, Volume 4, Issue 1, pp 21–29 | Cite as

Behaviour of rat-liver mitochondria during centrifugation in a sucrose gradient

  • Robert Wattiaux
Review Articles


One cannot centrifuge mitochondria in a sucrose gradient with impunity at any speed in any rotor. These subcellular structures are seriously deteriorated when the hydrostatic pressure they are exposed to in the centrifuge tube becomes too high. That can be shown by establishing the distributions of particle reference enzymes after centrifugation and by electron microscopic examination of the fractions. Our results illustrate that several factors are to be taken into account in isopycnic centrifugation experiments: the kind of rotor, the limits of the gradient, the temperature of centrifugation, the tissue from which the organelles originate.


Sucrose Hydrostatic Pressure Microscopic Examination Centrifuge Tube Sucrose Gradient 
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Copyright information

© Dr. W. Junk b.v. Publishers 1974

Authors and Affiliations

  • Robert Wattiaux
    • 1
  1. 1.Laboratoire de Chimie PhysiologiqueFacultés Universitaires Notre-Dame de la Paix. 61NamurBelgium

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