Abstract
A 29 kDa protein, isolated from the outer membrane ofCoxiella burnetii strain Nine Mile phase I by detergent Empigen BB, was characterized. The failure in removing lipopolysaccharides (LPS) from preparations of the protein by the purification method used indicates a strong binding between proteins and LPS in the outer membrane ofC. burnetii. The protein was immunogenic in mice and protected them against virulentC. burnetii challenge.
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References
Scott GH, Williams JC. Susceptibility ofCoxiella burnetii to chemical disinfectants. Ann NY Acad Sci 1990; 590: 291–296.
McCaul TF, Williams JC. Developmental cycle ofCoxiella burnetii: Structure and morphogenesis of vegetative and sporogenic differentiation. J Bacteriol 1981; 147: 1063–1076.
Hackstadt T. Steric hindrance of antibody binding to surface proteins ofCoxiella burnetii by phase I lipopolysaccharide. Infect Immun 1988; 56: 802–807.
Williams JC, Johnston MR, Peacock MG, Thomas LA, Stewart S, Portis JL. Monoclonal antibodies distinguish phase variants ofCoxiella burnetii. Infect Immun 1984; 43: 421–428.
Williams JC, Hoover TA, Waag DM, Banerjee-Bhatnagar N, Bolt CR, Scott GH. Antigenic structure ofCoxiella burnetii: A comparison of lipopolysaccharide and protein antigens as vaccines against Q fever. Ann NY Acad Sci 1990; 590: 370–380.
Müller HP, Schmeer N, Rantamäki L, Semler B, Krauss H. Isolation of a protein antigen fromCoxiella burnetii. Zbl Bakt Hyg A 1987; 265: 277–289.
Hendrix LR, Samuel JE, Mallavia LP. Identification and cloning of a 27 kDaCoxiella burnetii immunore-active protein. Ann NY Acad Sci 1991; 590: 534–540.
Hendrix LR, Mallavia LP, Samuel JE. Cloning and sequencing ofCoxiella burnetii outer membrane protein gene com 1. Infect Immun 1993; 61: 470–477.
Thompson HA, Bolt CR, Hoover T, Williams JC. Induction of heat-shock proteins inCoxiella burnetii. Ann NY Acad Sci 1991; 590: 127–135.
Lowell GH, Smith LF, Seid C, Zollinger WD. Peptides bound to proteosomes via hydrophobic feet become highly immunogenic without adjuvants. J. Exp Med 1988; 167: 658–663.
McCaul TF, Barnerjee-Bhatnagar N, Williams JC. Antigenic differences betweenCoxiella burnetii cells revealed by postembedding immunoelectron microscopy and immunoblotting. Infect Immun 1991; 59: 3243–3253.
Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principles of protein dye binding. Anal Biochem 1976; 72: 248–254.
Dubios M, Giles KR, Hamilton JK, Rebers PA, Smith F. Colorimetric method for determination of sugars and related substances. Anal Chem 1956; 28: 350–356.
Kazár J, Schramek Š. Relationship between delayed hypersensitivity reaction and resistance to virulent challenge in mice immunized with differentCoxiella burnetii antigenic preparation. In: Kazár J ed.Rickettsiae and rickettsial diseases. Proc 3rd Int Symp Publ Bratislava: House of the Slovak Academy of Sciences 1985; 282–288.
Gajdošová E, Brezina R. Cell-mediated immune response toCoxiella burnetii antigens in Q fever convalescents and vaccinees. Acta Virol 1989; 33: 474–481.
Kováčová E, Gallo J, Schramek Š, Kazár J, Brezina R.Coxiella burnetii antigens for detection of Q fever antibodies by ELISA in human sera. Acta Virol 1987; 31: 254–259.
Schramek Š, Brezina R, Kazár J. Influence of mild acid hydrolysis on the antigenic properties of phase ICoxiella burnetii. Acta Virol 1978; 22: 302–308.
Brezina R, Schramek Š, Kazár J, Urvölgyi J. Q fever chemovaccine for human use. Acta Virol 1974; 18: 269.
Kazár J, Schramek Š, Brezina R. Immunological properties of the lipopolysaccharide-protein complex ofCoxiella burnetii. Acta Virol 1978; 22: 309–315.
Lukáčová M, Brezina R, Schramek Š, Pastorek J. Chemical composition of phase ICoxiella burnetii soluble antigen prepared by trichloroacetic acid extraction. Acta Virol 1989; 33: 75–80.
Ormsbee RA. A method of purifyingCoxiella burnetii and other pathogenic rickettsiae. J Immunol 1962; 88: 100–108.
Laemmli UK. Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature (London) 1970; 227: 680–685.
Hitchcock PJ, Brown TM. Morphological heterogeneity amongSalmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J Bacteriol 1983; 154: 269–277.
Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc Natl Acad Sci 1979; 76: 4350–4354.
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Lukáčová, M., Gajdošová, E., Škultéty, L. et al. Characterization and protective effect of a 29 kDa protein isolated fromCoxiella burnetii by detergent Empigen BB. Eur J Epidemiol 10, 227–230 (1994). https://doi.org/10.1007/BF01730376
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DOI: https://doi.org/10.1007/BF01730376