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Human monoclonal antibody99mTc-88BV59: Detection of colorectal cancer, recurrent or metastatic disease and immunogenicity assessment

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Abstract

This study presents immunoscintigraphic results in 24 patients suffering from primary colorectal cancer, recurrent or metastatic disease after the injection of 1197–1351 MBq technetium-99m labelled totally human monoclonal antibody 88BV59. Labelling efficacy of99mTc-88BV59 ranged from 97% to 99%. Immunoscintigraphy was performed 18–20 h after injection. Scintigraphic findings were compared with those of computed tomography (CT). Patients underwent surgery in order to evaluate immunoscintigraphic findings histologically. Sera of the patients (before injection and 1 and 3 months post infusion) were analysed for the presence of human anti-human antibodies (HAHA). None of the patients showed a HAHA response as assessed by a solid-phase ELISA assay. The antibody scan detected about 25% more lesions than CT. In the detection of extrahepatic disease, the sensitivity of the antibody scan proved to be 68%, whereas the sensitivity of CT was 41%.

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Author notes

  1. Bernd J. Krause

    Present address: Department of Nuclear Medicine, Research Center Jülich, D-52425, Jülich, Germany

Authors and Affiliations

  1. Department of Nuclear Medicine, Johann Wolfgang Goethe University Medical Center, Theodortern-Kai 7, D-60590, Frankfurt/Main, Germany

    Bernd J. Krause, Richard P. Baum, Andreas Niesen & Gustav Hör

  2. Department of General and Abdominal Surgery, Johann Wolfgang Goethe University Medical Center, Frankfurt/Main, Germany

    Elisabeth Staib-Sebler & Matthias Lorenz

Authors
  1. Bernd J. Krause
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  2. Richard P. Baum
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  3. Elisabeth Staib-Sebler
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  4. Matthias Lorenz
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  5. Andreas Niesen
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  6. Gustav Hör
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Krause, B.J., Baum, R.P., Staib-Sebler, E. et al. Human monoclonal antibody99mTc-88BV59: Detection of colorectal cancer, recurrent or metastatic disease and immunogenicity assessment. Eur J Nucl Med 24, 72–75 (1997). https://doi.org/10.1007/BF01728312

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  • DOI: https://doi.org/10.1007/BF01728312

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