Klinische Wochenschrift

, Volume 66, Issue 4, pp 144–148 | Cite as

Lysosomale Enzymaktivität von Monozyten/Makrophagen nach Inkubation mit postprandialem fettreichem Serum und ihre Bedeutung in der Entstehung der Atherosklerose

  • K. Henze
  • G. Wolfram
Originalien
  • 24 Downloads

Abkürzungen

ACEH

Acid Cholesteryl Ester Hydrolase (EC 3.1.1.13)

HDL

high density lipoproteins

LDH

Lactat-Dehydrogenase (EC 1.1.1.27)

LDL

low density lipoproteins

NAGA

N-Acetyl-β-Glucosaminidase (EC 3.2.1.30)

VLDL

very low density lipoproteins

Lysosomal enzyme activity of monocyte-macrophages after incubation with postprandial hyperlipidemic serum and their role in atherogenesis

Summary

Lipid accumulation in macrophages is a prominent feature of the atherosclerotic lesion. Decreased lysosomal function of these cells might play an important role in the pathogenesis of the atherosclerotic foam cell. In this investigation six normal volunteers were fed a meal with a high fat content (68,9% energy, P/S ratio 0,13). The hyperlipidemic postprandial serum was incubated with monocyte derived macrophages. The enzyme activity of cathepsin B, acid cholesterylester-hydrolase and N-acetyl-β-hydrolase decreased significantly in these cells. Thus, inadequate response in enzyme activity of lysosomal enzymes in case of fat overload might contribute to the development of the atherosclerotic foam cell.

Key words

Macrophages Lysosomal enzyme activity Atherosclerosis Hyperlipidemic serum 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literatur

  1. 1.
    Ahrens EH, Insull W jr, Blomstrand R, Hirsch J, Tsattas TT, Peterson ML (1957) The influence of Dietary Fats on Serum Lipid Levels in Man. Lancet I:943–953Google Scholar
  2. 2.
    Barrett AJ, Kirschke H (1981) Cathepsin B, Cathepsin H and Cathepsin L. Methods Enzymol 80:535–561Google Scholar
  3. 3.
    Bates SR, Murphy LP, Fegn Z, Kanazawa T, Getz GS (1983) Very Low Density Lipoproteins Promote Triglyce-ride Accumulation in Macrophages. Arteriosclerosis 4:103–113Google Scholar
  4. 4.
    Boyüm A (1976) Isolation of Mononuclear Cells and Granulocytes from Human Blood. Isolation of Mononuclear Cells by one Centrifugation, and of Granulocytes by Combining Centrifugation and Sedimentation at 1 g. Scand J Clin Invest 58:1465–1474Google Scholar
  5. 5.
    Brecher P, Hae Yung Pyun, Chobanian AV (1977) Effect of Atherosclerosis on Lysosomal Cholesterol Esterase Activity in Rabbit Aorta. J Lipid Res 18:154–163Google Scholar
  6. 6.
    Burstein M, Scholnick HF, Martin R (1970) Rapid Methods for the Isolation of Lipoproteins from Human Serum by Precipitation with Polyanions. J Lipid Res 11:585–589Google Scholar
  7. 7.
    de Duve C (1974) The Participation of Lysosomes in the Transformation of Smooth Muscle Cells to Foamy Cells in the Aorta of Cholesterol-fed Rabbits. Acta Cardiol 20 (Suppl):9–25Google Scholar
  8. 8.
    Eggstein M, Kreutz FH (1966) Eine neue Bestimmung der Neutralfette im Blutserum und Gewebe. Klin Wochenschr 44:262–265Google Scholar
  9. 9.
    Fogelman AM, Seagers J, Hokom M, Edwards PA (1979) Separation of and Cholesterol Synthesis by Human Lymphocytes and Monocytes. J Lipid Res 20:379–388Google Scholar
  10. 10.
    Fowler S, Shio H, Wolinsky H (1977) Subcellular Fractionation and Morphology of Calf Aortic Smooth Muscle Cells. J Cell Biol 75:166–184Google Scholar
  11. 11.
    Gerrity RG (1981) The Role of the Monocyte in Atherogenesis. Transition of Blood-Borne Monocytes into Foam Cells in Fatty Lesions. Am J Path 25:181–190Google Scholar
  12. 12.
    Goldstein JL, Ho YK, Brown MS, Innerarity TL, Mahley RW (1980) Cholesterol Ester Accumulation in Macrophages Resulting from Receptor-Mediated Uptake and Degradation of Hypercholesterolemic Canine β-Very Low Density Lipoproteins. J Biol Chem 225:1839–1848Google Scholar
  13. 13.
    Henze K, Chait A (1981) Use of Circulating Mononuclear Cells to study the Effect of Metabolic Changes on Lysosomal Enzyme Activities and Low Density Lipoprotein Receptors: Effect of Insulin Therapy in Diabetic Patients. Diabetologia 20:625–629Google Scholar
  14. 14.
    Kenagy DR, Floren CH, Bierman EL, Kudchodkar B, Albers JJ (1982) Cholesterol Homeostasis of Skin Fibroblasts after Incubation with Postabsorptive and Postprandial Lipoproteins. The Effect of Fatty Meal. Arteriosclerosis 2:290–295Google Scholar
  15. 15.
    Kritschevsky D, Kothari HV (1978) Arterial Enzymes of Cholesteryl Ester Metabolism. Adv Lipid Res 16:221–266Google Scholar
  16. 16.
    Leake DS, Peters TJ (1981) Proteolytic Degradation of Low Density Lipoproteins by arterial Smooth Muscle Cells. The Role of Individual Cathepsins. Biochim Biophys Acta 664:108–116Google Scholar
  17. 17.
    Lowry OH, Rosebrough NJ, Farr AL, Randall JR (1951) Protein Measurement with the Folin Phenol Reagent. J Biol Chem 193:265–275Google Scholar
  18. 18.
    Mancini G, Carbonara AO, Heremans JF (1965) Immunochemical quantitation of antigens by single radial immundiffusion. Immunochemistry 2:235–254Google Scholar
  19. 19.
    Peters TJ, Müller M, de Duve C (1972) Lysosomes of the Arterial Wall. I. Isolation and Subcellular Fractionation of Cells from Normal Rabbit Aorta. J Exp Med 136:1117–1139Google Scholar
  20. 20.
    Röschlau P, Bernt E, Gruber W (1974) Enzymatische Bestimmung des Gesamt-Cholesterins im Serum. Z Klin Chem Klin Biochem 12:403–407Google Scholar
  21. 21.
    Sloan H, Fredrickson D (1972) Enzyme deficiency in Cholesterol Ester Storage Disease. J Clin Invest 51:1923–1926Google Scholar
  22. 22.
    St Clair RW, Greenspan P, Leight M (1983) Enhanced Cholesterol Delivery to Cells in Culture by Low Density Lipoproteins from Hypercholesterolemic Monkeys. Correlation of Cellular Cholesterol Accumulation with Low Density Lipoprotein Molecular Weight. Arteriosclerosis 3:77–86Google Scholar
  23. 23.
    Takano T, Black WJ, Peters TJ, de Duve C (1974) Assay Kinetics, and Lysosomal Localization of an Acid Cholesteryl Esterase in Rabbit Aortic Smooth Muscle Cells. J Biol Chem 249:6732–6737Google Scholar
  24. 24.
    Wolinsky H, Goldfisher S, Schiller B (1973) Lysosomes in Aortic Smooth Muscle Cells: Effects of Hypertension. Am J Pathol 73:727–734Google Scholar
  25. 25.
    Wolinsky H, Goldfisher S, Capron L (1978) Hydrolase Activities in the Rat Aorta. — Effects of Diabetes Mellitus and Insulin Treatment. Circ Res 42:821–830Google Scholar
  26. 26.
    Wolinsky H, Fowler S (1978) Participation of Lysosomes in Atherosclerosis. New Engl J Med 23:1173–1178Google Scholar
  27. 27.
    Zöllner N, Gröbner W, Berger Ch, Wolfram G (1969) Elektrophoretische Trennung der Serumlipoproteine im Agarosegel mit Albuminzusatz. Z klin Chem klin Bioch 7:525–529Google Scholar

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • K. Henze
    • 1
  • G. Wolfram
    • 1
  1. 1.Medizinische Poliklinik der Universität MünchenGermany

Personalised recommendations