Abstract
The sex chromosomal constitution has been determined in various types of human leukocytes at interphase by use of fluorescence in situ hybridization with X- and/or Y-specific DNA probes. It is found that during aging and differentiation of myelocytes into polymorphs there is no significant change in the relative frequency of various types of male and female cells with a specific type of sex chromosomal constitution. Nonrandom variability of the relative proximity between the X chromosomes within the nuclei is also observed in female cells. Moreover, we are the first to determine that sex-specific “drumsticks” and “sessile nodules” in female polymorphs originate from the X chromosomes and that non-sex-specific “drumstick-like” bodies in male polymorphs are of Y chromosomal origin.
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References
Barr ML, Bertram EG (1949) A morphological distinction between neurons of the male and female, and the behavior of the nucleolar satellite during accelerated nucleoprotein synthesis. Nature 163:676–677
Bennett MD (1983) The spatial distribution of chromosomes. In: Brandham PE, Bennett MD (eds) Kew Chromosome Conference II. George Allen & Unwin, London, pp 71–79
Comings DE (1980) Arrangement of chromatin in the interphase nucleus. Hum Genet 53:131–143
Cremer T, Cremer C, Baumann H, Leudtke EK, Sperling K, Teuber V, Zorn C (1982) Rabl's model of the interphase chromosome arrangement tested in Chinese hamster cells by premature chromosome condensation and laser UV-microbeam experiments. Hum Genet 60:46–56
Cremer T, Landegent J, Bruckner A, Scholl H, Schardin M, Hager H, Devilee P, Pearson P, Van der Ploeg M (1986) Detection of chromosome aberrations in the human interphase nucleus by visualization of specific target DNAs. Hum Genet 74:346–352
Davidson WM, Smith DR (1954) A morphological sex difference in polymorphonuclear neutrophil leukocytes. Br Med J 2:6–7
Dyer KA, Canfield TK, Gartler SM (1989) Molecular cytological differentiation of active from inactive X domains in interphase: implications for X chromosome inactivation. Cytogenet Cell Genet 50:116–120
Hens L, Baumann H, Cremer T, Sutter A, Cornelis JJ, Cremer C (1983) Immuno-cytochemical localization of chromatin regions UV-microirradiated in S-phase or anaphase: evidence for a territorial organization of chromosomes during the cell cycle of Chinese hamster cells. Exp Cell Res 149:257–269
Lamborot-Manzur M, Tishler PV, Atkin L (1971) Fluorescent drumsticks in male polymorphs. Lancet i:973–974
Manuelidis L (1985) Individual interphase chromosome domains revealed by in situ hybridization. Hum Genet 71:288–293
Mittwoch U (1964) Sex chromatin. J Med Genet 1:50–76
Mukherjee AB, SanSebastian J (1975) Fluorescent drumsticks in human female polymorphs. Br Med J 1:206–207
Mukherjee AB, SanSebastian J (1976) Fluorescent characteristics of drumsticks, drumstick-like projections, and other nuclear bodies in human blood cells. Cytogenet Cell Genet 16:468–478
Mukherjee AB, Blattner PY, Nitowski HM (1972a) Quinicrine fluorescence of sex chromatin in human amniotic fluid cell cultures. Nature 235:226–229
Mukherjee AB, Moser GC, Nitowski HM (1972b) Fluorescence of X and Y chromatin in human interphase cells. Cytogenetics 11:216–227
Pearson PL, Bobrow M, Vosa CG (1970) Technique for identifying Y chromosomes in human interphase nuclei. Nature 226:78–80
Pinkel D, Straume T, Gray JW (1986) Cytogenetic analysis using quantitative, high sensitivity, fluorescence hybridization. Proc Natl Acad Sci USA 83:2934–2938
Polani PE, Mutton DE (1971) Y-fluorescence of interphase nuclei, especially circulating lymphocytes. Br Med J 1:138–142
Rappold G, Cremer T, Hager H, Davies K, Muller C, Yang T (1984) Sex chromosome positions in human interphase nuclei as studied by in situ hybridization with chromosome specific DNA probes. Hum Genet 67:317–325
Ricci N, Gastoldi GL, Dallapiccola B, Baserga A (1971) Small drumsticks and long Y chromosomes. Br Med J 1:346–347
Schardin M, Cremer T, Hager HD, Lang M (1985) Specific staining of human chromosomes in Chinese hamster × man hybrid cell lines demonstrates interphase chromosome territories. Hum Genet 71:281–287
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Mukherjee, A.B., Parsa, N.Z. Determination of sex chromosomal constitution and chromosomal origin of drumsticks, drumstick-like structures, and other nuclear bodies in human blood cells at interphase by fluorescence in situ hybridization. Chromosoma 99, 432–435 (1990). https://doi.org/10.1007/BF01726695
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DOI: https://doi.org/10.1007/BF01726695