European Journal of Epidemiology

, Volume 11, Issue 4, pp 475–478 | Cite as

Prevalence ofLegionella pneumophila serogroup 1 antibodies in blood donors

  • Laura Franzin
  • Florinda Scramuzza
Brief Report


The prevalence of anti-Legionella pneumophila serogroup 1 antibodies in 777 blood donors of the Turin area was determined by the indirect immunofluorescence assay (IFA) and by the microagglutination test (MA). Low titers (IFA of 1/16 and MA of 1/8) were found in 0.3% of the subjects. A statistically significant difference was not observed by sex and by age for IFA titers, but was noted by sex for MA titers of ⩾1/4 (p<0.05). The upper limit of normal titer was <1/8 by IFA and <1/4 by MA at 15% cutoff level and 1/8 by IFA and 1/4 by MA at 1% cutoff level. In conclusion, the prevalence of antibodies in the Turin area was very low; IFA titers of ⩾1/64 and MA titers of >1/16 can be considered as presumptive of infection in a single serum specimen of a patient with pneumonia; no change in the epidemiology of the disease was observed in the recent years.

Key words

Antibodies Blood donors Legionella pneumophila 


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  1. 1.
    Wilkinson HW, Fikes BJ, Cruce DD. Indirect immunofluorescence test for serodiagnosis of Legionnaires disease: Evidence for serogroup diversity of Legionnaires disease bacterial antigens and for multiple specificity of human antibodies. J Clin Microbiol 1979; 9: 379–383.Google Scholar
  2. 2.
    Farshy CE, Klein GC, Feeley JC. Detection of antibodies to Legionnaires' disease organism by microagglutination and microenzyme immunosorbent assay tests. J Clin Microbiol 1978; 7: 327–331.Google Scholar
  3. 3.
    Harrison TG, Taylor AG. A rapid microagglutination test for the diagnosis ofLegionella pneumophila (serogroup 1) infection. J Clin Pathol 1982; 35: 1028–1031.Google Scholar
  4. 4.
    Castellani Pastoris M, Ciarrocchi S, Di Capua A, Temperanza AM. Comparison of phenol- and heat-killed antigens in the indirect immunofluorescence test for serodiagnosis ofLegionella pneumophila group 1 infections. J Clin Microbiol 1984; 20: 780–783.Google Scholar
  5. 5.
    Temperanza AM, Di Capua A, Ciarrocchi S, Ciceroni L, Castellani Pastoris M. More experience on the microagglutination test in the diagnosis ofLegionella pneumophila infection. Microbiologica 1986; 9: 71–79.Google Scholar
  6. 6.
    Glants SA (ed). Statistica per discipline biomediche, 2nd ed. Milan: Mc Graw-Hill/Libri Italia, 1988: 100–102Google Scholar
  7. 7.
    Wilkinson HW, Cruce DD, Broome CV. Validation ofLegionella pneumophila indirect immunofluorescence assay with epidemic sera. J Clin Microbiol 1981; 13: 139–146.Google Scholar
  8. 8.
    Wilkinson HW (ed). Hospital-laboratory diagnosis ofLegionella infections. Atlanta, GA: Centers for Disease Control, 1987: 23–25.Google Scholar
  9. 9.
    Harrison TG, Dournon E, Taylor AG. Evaluation of sensitivity of two serological tests for diagnosing pneumonia caused byLegionella pneumophila 1. J. Clin Pathol 1987; 40: 77–82.Google Scholar
  10. 10.
    Bornstein N, Fleurette J. Comparison of microagglutination with indirect immunofluorescence assay for the diagnosis of infection withLegionella pneumophila serogroup 1. Eur J Clin Microbiol Infect Dis 1983; 2: 335–339.Google Scholar
  11. 11.
    Helms CM, Johnson W, Renner ED, Hierholzer WJ Jr, Wintermeyer LA, Viner JP. Background prevalence of microagglutination antibodies toLegionella pneumophila serogroups 1, 2, 3 and 4. Inf 1mm 1980; 30: 612–614.Google Scholar
  12. 12.
    Harrison TG, Taylor AG (eds). A laboratory manual forLegionella. Chichester: John Wiley & Sons, 1988: 113–135.Google Scholar
  13. 13.
    Taylor AG, Harrison TG. Serological tests forLegionella pneumophila serogroup 1 infections. Zentr Bakteriol Mikrobiol Hyg (A) 1983; 255: 20–26.Google Scholar
  14. 14.
    Taylor AG, Harrison TG, Burè A, Dournon E. Validation of serological tests for Legionnaires diseases by using sera from bacteriologically proven and epidemiological associated cases. In: Thornsberry C, Balows A, Feeley JC, Jakubowski W (eds),Legionella: Proceedings of the International Symposium. Washington, DC: American Society for Microbiology, 1984: 31–32.Google Scholar
  15. 15.
    Franzin L, Scramuzza F, Gioannini P, Fora R, Croce M, Bombonati R. Risultati preliminari di una epidemia di legionellosi in turisti. Microbiol Med 1994; 9: 127–129.Google Scholar
  16. 16.
    Bornstein N, Janin N, Bourguignon G, Surgot M, Fleurette J. Prevalence of anti-Legionella antibodies in a healthy population and patients with tuberculosis or pneumonia. Path Biol 1987; 35: 353–356.Google Scholar
  17. 17.
    Boswell TCJ, Kudesia G. Seropositivity forLegionella in Campylobacter infection. Lancet 1992; 339: 191.Google Scholar
  18. 18.
    Boswell TCJ, Marshall LE, Kudesia G. Crossreactions betweenLegionella and Campylobacter spp. Lancet 1992; 340: 551.Google Scholar
  19. 19.
    Wilkinson HW, Reingold AL, Brake BJ, McGiboney DL, Gorman GW, Broome CV. Reactivity of serum from patients with suspected Legionellosis against 29 antigens of Legionellaceae and Legionella-like organisms by indirect immunofluorescence assay. J Infect Dis 1983; 147: 23–31.Google Scholar
  20. 20.
    Smalley DL, Ourth DD. Seroepidemiology ofLegionella pneumophila: A study of adults form Memphis, Tennessee, USA. Am J Clin Pathol 1981; 75: 201–203.Google Scholar
  21. 21.
    Di Capua A, Temperanza AM, Ciarrocchi S. Castellani Pastoris M. Upper limit of normal titer forLegionella pneumophila group 1 by indirect immunofluorescence and microagglutination tests in healthy population in Italy. Boll Ist Sier Mil 1985; 64: 475–478.Google Scholar
  22. 22.
    WHO. Epidemiology, prevention and control of legionellosis. Memorandum from a WHO meeting. Bull WHO 1990; 68: 155–168.Google Scholar

Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • Laura Franzin
    • 1
  • Florinda Scramuzza
    • 1
  1. 1.Infectious Diseases InstituteUniversity of TurinTurinItaly

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