Abstract
Samples of peripheral blood mononuclear cells (PBMC) were collected during 1990–91 from seropositive healthy, male HIV-1 carriers visiting Taipei Venereal Disease Control Center, and a male AIDS patient admitted to a general hospital. The V3 and its flanking nucleotide (nt) sequences in their DNA were amplified by polymerase chain reaction (PCR) and compared with those of known HIV-1 prototypes. The nt sequences obtained from 21 individuals (e.g., TW92) clustered as Group A, which were highly homologous (95.6–99.5%) to that of HXB2 virus while those from 6 individuals (TW90, TW91, TW97, TW99, TW102 and TW104) were classified as Group B showing low similarities (73.2–84.2%) to those of HXB2 and moderate similarities (80.7–90.0%) to those of SC and Bangkok (BK) viruses. By comparison of their deduced amino acid sequences with those of consensus sequences for subtypes A-F as defined by Myers et al. (1993), both Groups A and B viruses (except TW102) together with those of HXB2, SC and BK viruses could be identified as members or variants of subtype B, and the TW102 virus as a member of subtype E viruses. Individuals with the Group A viruses included 4 homosexual and 17 heterosexual Taiwanese males, 2 of the latter having a history of I.V. drug abuse. Among individuals with Group B viruses, those with TW97, TW99, TW104 and TW91, who was an AIDS patient, were heterosexual Taiwanese males, whereas both TW90 and TW102 viruses were from individuals who were overseas heterosexual Chinese from Thailand, the former with a history of I.V. drug abuse and the latter without.
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Chang P-Y, Lin K-C, Chuang C-Y, Wang C-R, Chen M-Y, Hsieh W-C, Wang G-R, Cheng D-L, Chang KSS, Ko Y-C, Lin H-T, Huang CCH. Status and trend of HIV-1 infection and AIDS in Taiwan, December, 1991. Asian Pacific J Allergy & Immunol 1992; 10: 65–68.
Dickman S. AIDS threatens Asia. Nature 1991; 351: 682.
Guo HG, Chang KSS. A vertically transmitted HIV-1gag-subtype variant detected in Taiwan. Jpn J Med Sci Biol 1992; 45: 127–136.
Guo HG, Reitz MS, Gallo RC, Ko YC, Chang KSS. A new subtype of HIV-1gag sequence detected in Taiwan. AIDS Res & Human Retroviruses 1993; 9: 925–927.
Hwang SS, Boyle TJ, Lyerly HK, Cullen BR. Identification of the envelope V3 loop as the primary determinant of cell tropism in HIV-1. Science 1991; 253; 71–74.
LaRosa GJ, Davide JP, Weinhold K, Waterbury JA, Profy AT, Lewis JA, Langlois AJ, Dreesman GR, Boswell RN, Shadduck P, Holley LH, Karplus M, Bolognesi DP, Matthews TJ, Emini EA, Putney SD. Conserved sequence and structural elements in the HIV-1 principal neutralizing determinant. Science 1990; 249: 932–935
McCutchan FE, Hegerich PA, Brennan TP, Phanuphak P, Singharaj P, Jugsudee A, Berman PW, Gray AM, Fowler AK, Burke DS. Genetic variants of HIV-1 in Thailand. AIDS Res & Human Retroviruses 1992; 8: 1887–1895.
Meloen RH, Liskamp RM, Goudsmit J. Specificity and function of the individual amino acids of an important determinant of human immunodeficiency virus type 1 that induced neutralizing antibody. J Gen Virol 1989; 70: 1505–1512.
Myers G, Korber B, Berzofsky JA, Smith RF, Pavlakis GN, Wain-Hobson S. Human Retroviruses and AIDS 1993. Theoretical Biology and Biophysics Group, Los Alamos National Laboratory, Los Alamos, NM, 1993.
Ou C-Y, Takebe Y, Weniger BG, Luo C-C, Kalish ML, Auwanit W, Yamazaki S, Gayle HD, Young NL. Independent introduction of two major HIV-1 genotypes into distinct high-risk populations in Thailand. Lancet 1993; 341: 1171–1174.
Wolinsky SM, Wike CM, Korber BTM, Hutto C, Parks WP, Rosenblum LL, Kunstman KJ, Furtado MR, Muñoz JL. Selective transmission of human immunodeficiency virus type-1 variants from mothers to infants. Science 1992; 255: 1134–1137.
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Chang, K.S.S., Lin, CI., Ling, P. et al. Molecular subtypes ofenv sequences around V3 region of human immunodeficiency virus type 1 in Taiwan. Eur J Epidemiol 10, 247–250 (1994). https://doi.org/10.1007/BF01719345
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DOI: https://doi.org/10.1007/BF01719345