Abstract
Most of theCoxiella burnetii isolates (72 of 80) available in our institute share a 2.1 kbNotI fragment. Sequence analysis revealed two incomplete open reading frames (ORF) for putative geneshemA andbcR coding for glutamyl-tRNA-reductase and bicyclomycin resistance protein, respectively. Upon completing the ORF for thehemA gene by sequencing the adjacentNotI/EcoRI fragment a third ORF for the putativeprfA gene was determined coding for release factor 1, an enzyme catalysing the last step in protein biosynthesis.
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References
Thiele D, Willems H. Is plasmid based differentiation ofCoxiella burnetii in ‘acute’ and ‘chronic’ isolates still valid? Eur J Epidemiol 1994; 10: 427–434.
Atzpodien E, Baumgärtner W, Artelt A, Thiele D. Valvular endocarditis occurs as a part of a disseminatedCoxiella burnetii infection in immunocompromised BALB/cJ (H-2d) mice infected with the Nine Mile isolate ofC. burnetii. J Infect Dis ; 170: 223–226.
Heinzen RA, Mallavia LP. Cloning and functional expression of theCoxiella burnetii citrate synthase gene in Escherichia coli. Infect Immun 1986; 55: 848–855.
Thiele D, Willems H, Haas M, Krauss H. Analysis of the entire nucleotide sequence of the cryptic plasmid QpH1 fromCoxiella burnetii. Eur J Epidemiol 1994; 10: 413–420.
Sawadogo M, van Dyke MM. A rapid method for the purification of deprotected oligodeoxynucleotides. Nucl Acids Res 1993; 39: 674.
Arens M. Vermehrung vonCoxiella burnetii durch persistierende Infektion in Buffalo-Green-Monkey(BGM)-Zellkulturen. Zbl Vet Med 1983; B30: 109–116.
Thiele D, Willems H, Köpf G, Krauss H. Polymorphism in DNA restriction patterns ofCoxiella burnetii isolates investigated by pulsed field gel electrophoresis and image analysis. Eur J Epidemiol 1993; 9: 419–425.
Bentley J, Hyatt LS, Ainley K, Parish JH, Herbert RB, White GR. Cloning and sequence analysis of anEscherichia coli gene conferring bicyclomycin resistance. Gene 1993; 127: 117–120.
Ryden M, Murphy J, Martin R, Isaksson L, Gallant J. Structure of theBradyrhizobium japonicum hemA encoding 5-aminolevulinic acid synthase. Gene 1986; 54: 133–139.
Klein HA, Capecchi MR. Polypeptide chain termination. J Biol Chem 1971; 246: 1055–1061.
Caskey T, Leder P, Moldave K, Schlessinger D. Translation: Its mechanism and control. Science 1972; 176: 195–197.
Elliott T, Avissar YJ, Rhie G, Beale SI. Cloning and sequence of theSalmonella typhimurium hemL gene and identification of the missing enzyme in hemL mutants as glutamate-1-semialdehyde aminotrans-ferase. J Bacteriol 1990; 172: 7071–7084.
Robertson McClung C, Somerville JE, Guerinot ML, Chelm BK. Structure of theBradyrhizobium japonicum hemA encoding 5-aminolevulinic acid synthase. Gene 1987; 54: 133–139.
Tai T, Moore MD, Kaplan S. Cloning and characterization of the 5-aminolevulinate synthase gene(s) fromRhodobacter sphaeroides. Gene 1988; 70: 139–151.
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Willems, H., Thiele, D. & Krauss, H. Sequencing and linkage analysis of aCoxiella burnetii 2.1 kbNotI fragment. Eur J Epidemiol 11, 559–561 (1995). https://doi.org/10.1007/BF01719308
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DOI: https://doi.org/10.1007/BF01719308