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Evaluation of non-radioactive temperature gradient SSCP analysis and of temperature gradient gel electrophoresis for the detection of HPV 6-variants in condylomata acuminata and Buschke-Loewenstein tumours

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Abstract

A modified non-radioactive single strand conformation polymorphism analysis incorporating a temperature gradient (TG-SSCP) and temperature gradient gel electrophoresis (TGGE) were evaluated for the detection of human papillomavirus type 6 (HPV 6)-variants in 41 condylomata acuminata and 5 Buschke-Loewenstein tumours. TG-SSCP and TGGE analysed part of the transforming ORF E6 of HPV 6 spanning nucleotides 10 to 495. TG-SSCP distinguished between 8 HPV 6-variants whereas TGGE demonstrated 6 different DNA-species. HPV 6-strains found in Buschke-Loewenstein tumours did not vary in the analysed portion of the E6 ORF as compared to ordinary condylomata acuminata. TG-SSCP and TGGE further showed absence of double infection with different HPV 6-strains in the analysed samples. Our results demonstrated that both methods may be successfully used for the detection of different strains of microbiological agents, although TG-SSCP seemed to provide easier execution and to confer a higher degree of flexibility than TGGE.

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Authors and Affiliations

  1. Department of Dermatology, RWTH-Aachen, Aachen, Germany

    Albert Rübben, Claudia Traidl, Jens Malte Baron & Elke-Ingrid Grußendorf-Conen

Authors
  1. Albert Rübben
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  2. Claudia Traidl
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  3. Jens Malte Baron
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  4. Elke-Ingrid Grußendorf-Conen
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Correspondence to Albert Rübben.

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Rübben, A., Traidl, C., Baron, J.M. et al. Evaluation of non-radioactive temperature gradient SSCP analysis and of temperature gradient gel electrophoresis for the detection of HPV 6-variants in condylomata acuminata and Buschke-Loewenstein tumours. Eur J Epidemiol 11, 501–506 (1995). https://doi.org/10.1007/BF01719300

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  • DOI: https://doi.org/10.1007/BF01719300

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