Abstract
The T18d (formerlyT13 c) gene of BALB/c mice belongs to the category ofTla genes which is expressed by both thymocytes and TL+ T-cell leukemias. To elucidate the regulation of T18d, different restriction fragments of the 5' flanking region between -457 and+ 146 were linked to the chloramphenicol acetyltransferase (CAT) gene and transfected into TL+ and TL- cells. By comparison of transiently expresssedCAT activity among cells transfected with differentCAT constructs, the results suggest that determination of TL+ vs TL phenotypes is located within the region -105 to - 33, and that an element essential to T18 d expression resides within the region - 33 to + 54. Putative DNA-binding factors characterizing particular cell types and displaying selective affinity for particular TI 8d restriction fragments were identified by electrophoretic mobility shift assays with nuclear extracts (NEs). Two factors (or complexes) which bound specifically to the T18d fragment-105 to - 33 were expressed preferentially in TL+ cells and thus may be involved in determining the tissue-selective expression of TI8d. The close proximity of negative and positive cis-acting elements within the promoter region is consistent with regulation of T18d gene expression by a variety of trans-acting factors whose production is attuned to development and differentiation. The data provided may serve as a guide to study the regulation of other categories of Tla genes that are normally silent in thymocytes but may become expressed by leukemia cells.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Boyse, E. A.: The biology of Tla.Cell 38: 1–2, 1984
Chorney, M. J., Mashimo, H., Bushkin, Y., and Nathenson, S.G.: Characterization of the thymus leukemia (TL) product encoded by the BALB/c T3° gene by DNA-mediated gene transfer. Comparison to the T13° product and BALB/c leukemia TL.J Immunol 143: 3762–3768, 1989
Dignam, J. D., Lebovitz, R. M., and Roeder, R. G.: Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.Nucleic Acids Res 11: 1475–1489, 1983
Fisher, D. A., Hunt, S. W., III, and Hood, L.: Structure of a gene encoding a murine thymus leukemia antigen, and organization of Tla genes in the BALB/c mouse.J Exp Med 162: 528–545, 1985
Goodenow, R. S., McMillan, M., Nicolson, M., Nicolson B. T., Eakle, K., Davidson, N., and Hood, L.: Identification of the class I genes of the mouse major histocompatibility complex by DNA-mediated gene transfer.Nature 300: 231–237, 1982
Gorman, C. M.: High efficiency gene transfer into mammalian cells. In D. M. Glover (ed.): DNA Cloning, Vol 2, pp. 143–190, IRL, Oxford, 1985
Gorman, C. M., Moffat L. F., and Howard, B. H.: Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.Mol Cell Biol 2: 1044–1051, 1982
Hamasima, N., Takahashi, T., Taguchi, O., Nishizuka, Y., Stockert, E., Old, L. J., and Obata, Y.: Expression of TL, H-2, and chimeric H-2/TL genes in transgenic mice: abnormal thymic differentiation and T-cell lymphomas in a TL transgenic strain.Proc Natl Acad Sci USA 86: 7995–7999, 1989
Israel, A., Kimura, A., Fournier, A., Fellous, M., and Kourilsky, P.: Interferon response sequence potentiates activity of an enhancer in the promoter region of a mouse H-2 gene.Nature 322: 743–746, 1986
Kirmura, A., Israel, A., Bail, O. L., and Kourilsky, P.: Detailed analysis of the mouse H-2Kb promoter: enhancer-like sequences and their role in the regulation of class I gene expression.Cell 44: 261–272, 1986
Levy, D. E., Kessler, D. S., Pine, R., Reich, N., and Darnell, J. E., Jr: Interferon-induced nuclear factors that bind a shared promoter element correlate with positive and negative transcriptional control.Genes Dev 2: 383–393, 1988
Maniatis, T., Fritsch, E. F., and Sambrook, J.:Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 1982
Martin, L. H., Calabi, F., Lefebvre, F.-A., Bilsland, C. A. G., and Milstein, C.: Structure and expression of the human thymcyte antigens CDla, CDlb, and CDlc.Proc Natl Acad Sci USA 84: 9189–9193, 1987
Obata, Y., Stockert, E., Chen, Y.-T., Takahashi, T., and Old, L. J.: Influence of 5' flanking sequences on TL and H-2 exression in transfected L cellsProc Natl Acad Sci USA 85: 3541–3545, 1988
Sambrook, J., Fritsch, E. F., and Maniatis, T.:Molecular Cloning: A Laboratory Manual, 2nd edn, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 1989
Singh, H., Sen, R., Baltimore, D., and Sharp, P. A.: A nuclear factor that binds to a conserved sequence motif in transcriptional control elements of immunoglobulin genes.Nature 319: 154–158, 1986
Smale, S. T. and Baltimore, D.: The “Initiator” as a transcription control element.Cell 57: 103–113, 1989
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Horie, M., Matsuura, A., Chang, KJ. et al. Properties of the promoter region of theT18 d (T13 c)Tla gene. Immunogenetics 33, 171–177 (1991). https://doi.org/10.1007/BF01719236
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01719236