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The complete nucleotide sequence of bean yellow mosaic potyvirus RNA

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Summary

The complete nucleotide sequence of an Australian strain of bean yellow mosaic virus (BYMV-S) has been determined from cloned viral cDNAs. The BYMV-S genome is 9 547 nucleotides in length excluding a poly(A) tail. Computer analysis of the sequence revealed a single long open reading frame (ORF) of 9 168 nucleotides, commencing at position 206 and terminating with UAG at position 9 374–6. The ORF potentially encodes a polyprotein of 3 056 amino acids with a deduced Mr of 347 409. The 5′ and 3′ untranslated regions are 205 and 174 nucleotides in length respectively. Alignment of the amino acid sequence of the BYMV-S polyprotein with those of other potyviruses identified nine putative proteolytic cleavage sites. The predicted consensus cleavage site of the BYMV NIa protease was found to differ from that described for other potyviruses. Processing of the BYMV polyprotein at the designated proteolytic cleavage sites would result in a typical potyviral genome arrangement. The amino acid sequences of the putative BYMV encoded proteins were compared to the homologous gene products of twelve individual potyviruses to identify overall and specific regions of amino acid sequence homology.

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Authors and Affiliations

  1. Department of Microbiology, Monash University, Clayton, Melbourne, Victoria, Australia

    K. J. Guyatt, D. F. Proll, A. Menssen & A. D. Davidson

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  1. K. J. Guyatt
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  2. D. F. Proll
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  3. A. Menssen
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  4. A. D. Davidson
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Additional information

The nucleotide sequence data reported in this paper has been submitted to GenBank nucleotide sequence database and has been assigned the accession number U47033.

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Guyatt, K.J., Proll, D.F., Menssen, A. et al. The complete nucleotide sequence of bean yellow mosaic potyvirus RNA. Archives of Virology 141, 1231–1246 (1996). https://doi.org/10.1007/BF01718827

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  • DOI: https://doi.org/10.1007/BF01718827

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