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Comparison of the nucleotide sequences of the 3′-terminal regions of one aphid and two non-aphid transmissible isolates of potato A potyvirus

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Summary

The sequences of the 3′-terminal 1145 nucleotides of two non-aphid transmissible (NAT) isolates (Ali and Juliniere) and one aphid transmissible (AT) isolate (Rouge) of potato virus A (PVA) RNA were determined. Those sequences contained the complete coding region of the coat protein (CP) followed by a 3′-nontranslated region (3′-NTR) of 225 (Ali and Juliniere) and 227 (Rouge) nucleotides. The obtained sequences were compared to those of the 3'-regions of four published PVA isolates and a virus described as tamarillo mosaic virus (TamMV) which on the basis of sequence data is a strain of PVA. The analysis of the 3′-terminal region of PVA isolates indicated that the CP N-terminal variable domain (32 residues) divides PVA isolates into two subgroups, where only tripeptide DAG correlates with aphid transmissibility. In addition to DAG/DAS sequence we found four other amino acids at the N-terminus of PVA CP, which are conserved in two subgroups. The central region (core part and C-termini) of CP is highly conserved among all PVA isolates (96.6 to 99.6%). 3′-NTR, separates PVA isolates into two subgroups on the basis of its length and homology.

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Authors and Affiliations

  1. Institute of Chemical Physics and Biophysics, Tallinn, Estonia

    J. Andrejeva, A. Merits & L. Järvekülg

  2. Federal Centre for Breeding Research on Cultivated Plants, Institute for Pathogen Diagnostics, Aschersleben, Germany

    F. Rabenstein

  3. Institute of Biotechnology, University of Helsinki, Helsinki, Finland

    Ü. Puurand

Authors
  1. J. Andrejeva
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  2. A. Merits
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  3. F. Rabenstein
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  4. Ü. Puurand
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  5. L. Järvekülg
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Andrejeva, J., Merits, A., Rabenstein, F. et al. Comparison of the nucleotide sequences of the 3′-terminal regions of one aphid and two non-aphid transmissible isolates of potato A potyvirus. Archives of Virology 141, 1207–1219 (1996). https://doi.org/10.1007/BF01718825

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  • DOI: https://doi.org/10.1007/BF01718825

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