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Immunological response of mice to the bovine respiratory syncytial virus fusion glycoprotein expressed in recombinant baculovirus infected insect cells

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Summary

Bovine respiratory syncytial virus (BRSV) is a major cause of respiratory disease in calves. The BRSV genome encodes two major glycoproteins, G and F, which are the major targets for the host antibody response. We have expressed the F glycoprotein in insect cells (Sf9) using a recombinant baculovirus vector. A comparison of the F protein expressed in mammalian and insect cells by SDS-PAGE showed that only part of the baculovirus-produced protein was soluble and processed like the native protein. The antigenicity of the soluble form of the F protein expressed in insect cells was identical to that of the F protein expressed in mammalian cells. Immunization with the F protein expressed in insect cells induced neutralizing antibodies in mice. This antigenic preparation adjuvanted with Quil-A produced an increased neutralizing antibody titer and induced protection.

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Author notes

  1. K. Walravens

    Present address: Institut National de Recherche Vétérinaire, Bruxelles, Belgium

Authors and Affiliations

  1. Laboratoire de Microbiologie et d'lmmunologie, Facultés Universitaires Notre-Dame de la Paix (F.U.N.D.P.), Namur, Belgium

    K. Walravens, J. P. Matheise, I. Knott, C. Didembourg & J.-J. Letesson

  2. Division d'lmmunologie Animale, Centre d'Economie Rurale, Marloie, Belgium

    P. Coppe, A. Collard & F. Dessy

  3. Laboratoire de Biologie Moléculaire et de Physiologie Animale, Faculté des Sciences Agronomiques, Gembloux, Belgium

    R. Kettmann

Authors
  1. K. Walravens
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  2. J. P. Matheise
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  3. I. Knott
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  4. P. Coppe
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  5. A. Collard
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  8. R. Kettmann
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  9. J.-J. Letesson
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Walravens, K., Matheise, J.P., Knott, I. et al. Immunological response of mice to the bovine respiratory syncytial virus fusion glycoprotein expressed in recombinant baculovirus infected insect cells. Archives of Virology 141, 2313–2326 (1996). https://doi.org/10.1007/BF01718633

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  • DOI: https://doi.org/10.1007/BF01718633

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