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Determination of the 5′ and 3′ terminal noncoding sequences of the bi-segmented genome of the avibirnavirus infectious bursal disease virus

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Summary

Terminal sequences of the bi-segmented dsRNA genome of 3 different strains of infectious bursal disease virus (IBDV) were analyzed by the rapid amplification of cDNA 5′ ends (5′RACE) procedure. Both segments are 85% homologous in a 32-nucleotide sequence comprising the 5′ end, whereas the 3′ end has a conserved pentamer. Comparison to published terminal sequences of other IBDV strains revealed high conservation between the two segments but more serotype-specific nucleotide changes (5 on segment A and 3 on segment B) in the 5′ noncoding region compared to the 3′ noncoding region (none on segment A and 1 on segment B).

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Authors and Affiliations

  1. Department of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, Canada

    F. S. B. Kibenge, M. M. Nagarajan & B. Qian

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  1. F. S. B. Kibenge
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  2. M. M. Nagarajan
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  3. B. Qian
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Kibenge, F.S.B., Nagarajan, M.M. & Qian, B. Determination of the 5′ and 3′ terminal noncoding sequences of the bi-segmented genome of the avibirnavirus infectious bursal disease virus. Archives of Virology 141, 1133–1141 (1996). https://doi.org/10.1007/BF01718616

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  • DOI: https://doi.org/10.1007/BF01718616

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