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Prevalence of G and P serotypes among equine rotaviruses in the faeces of diarrhoeic foals

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Summary

Variant types of VP4 and VP7 gene segments of faecal rotaviruses from diarrhoeic foals were identified by restriction endonuclease digestion of reverse transcription/polymerase chain reaction (RT/PCR) products. The variants observed were correlated with serotypes by determination of the sequence of representative RT/PCR products (entire coding sequence for VP7 and the VP8 region of VP4) and comparison to published sequences of equine G and P serotype genes. Both G and P serotypes could be predicted for 95/116 (82%) strains, P serotype only for a further 8 (7%) strains and G serotype only for 1 (1%) strain. All characterised strains belonged to the same P serotype, P12, although minor sequence variations were observed. Of those strains able to be assigned to G serotypes, 84/96 (87.5%) belonged to serotype G3A, and 12/96 (12.5%) belonged to serotype G14. Comparison of G serotyping by ELISA to the RT/PCR method showed that serotyping equine rotaviruses by currently available ELISA methods was prone to error. This study establishes the restricted serotypic diversity of equine rotaviruses, and the significance of serotype G14.

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References

  1. Bern C, Unicomb L, Gentsch JR, Banul N, Yunus M, Sack RB, Glass RI (1992) Rotavirus diarrhea in Bangladeshi children: correlation of disease severity with serotypes. J Clin Microbiol 30: 3234–3238

    Google Scholar 

  2. Browning GF, Chalmers RM, Fitzgerald TA, Corley KT, Campbell I, Snodgrass DR (1992) Rotavirus serotype G3 predominates in horses. J Clin Microbiol 30: 59–62

    Google Scholar 

  3. Browning GF, Chalmers RM, Fitzgerald TA, Snodgrass DR (1991) Serological and genomic characterization of L338, a novel equine group A rotavirus G serotype. J Gen Virol 72: 1059–1064

    Google Scholar 

  4. Browning GF, Chalmers RM, Fitzgerald TA, Snodgrass DR (1992) Evidence for two serotype G3 subtypes among equine rotaviruses. J Clin Microbiol 30: 485–491

    Google Scholar 

  5. Browning GF, Chalmers RM, Snodgrass DR, Batt RM, Hart CA, Ormarod SE, Leadon D, Stoneham SJ, Rossdale PD (1991) Prevalence of enteric pathogens in diarrhoeic Thoroughbred foals in Britain and Ireland. Equine Vet J 23: 405–409

    Google Scholar 

  6. Browning GF, Fitzgerald TA, Chalmers RM, Snodgrass DR (1991) A novel group A rotavirus G serotype: serological and genomic characterization of equine isolate FI23. J Clin Microbiol 29: 2043–2046

    Google Scholar 

  7. Browning GF, Sykes JE, Huntington PJ, Hollywell CA, Begg AP (1992) Rotavirus infections in Australian foals. Aust Equine Vet 10: 123–126

    Google Scholar 

  8. Ciarlet M, Reggeti F, Pina CI, Liprandi F (1994) Equine rotaviruses with G14 serotype specificity circulate among venezuelan horses. J Clin Microbiol 32: 2609–2612

    Google Scholar 

  9. Dwyer RM, Powell DG, Lyons F, Donahue JM, Roberts AW, Osborne M (1988) The aetiology of infectious diarrhoea in Thoroughbred foals in central Kentucky. Equine Vet J [Suppl] 5: 59–60

    Google Scholar 

  10. Fitzgerald TA, Browning GF (1991) Increased sensitivity of a rotavirus serotyping enzymelinked immunosorbent assay by the incorporation of CaCl2. J Virol Methods 33: 299–304

    Google Scholar 

  11. Gentsch JR, Glass RI, Woods P, Gouvea V, Gorziglia M, Flores J, Das BK, Bhan MK (1992) Identification of group A rotavirus gene 4 types by polymerase chain reaction. J Clin Microbiol 30: 1365–1373

    Google Scholar 

  12. Gerna G, Sarasini A, Arista S, di Matteo A, Giovannelli L, Parea M, Halonen P (1990) Prevalence of human rotavirus serotypes in some European countries 1981–1988. Scand J Infect Dis 22: 5–10

    Google Scholar 

  13. Gerna G, Sears J, Hoshino Y, Steele AD, Nakagomi O, Sarasini A, Flores J (1994) Identification of a new VP4 serotype of human rotaviruses. Virology 200: 66–71

    Google Scholar 

  14. Gomez J, Estes MK, Matson DO, Bellinzoni R, Alvarez A, Grinstein S (1990) Serotyping of human rotaviruses in Argentina by ELISA with monoclonal antibodies. Arch Virol 112: 249–259

    Google Scholar 

  15. Gorziglia M, Larralde G, Kapikian AZ, Chanock RM (1990) Antigenic relationships among human rotaviruses as determined by outer capsid protein VP4. Proc Natl Acad Sci USA 87: 7155–7159

    Google Scholar 

  16. Gouvea V, Glass RI, Woods P, Taniguchi K, Clark HF, Forrester B, Fang ZY (1990) Polymerase chain reaction amplification and typing of rotavirus nucleic acid from stool specimens. J Clin Microbiol 28: 276–282

    Google Scholar 

  17. Gouvea V, Santos N, Timenetsky MC (1994) Identification of bovine and porcine rotavirus G types by PCR. J Clin Microbiol 32: 1338–1340

    Google Scholar 

  18. Greenberg HB, Valdesuso J, van Wyke K, Midthun K, Walsh M, McAuliffe V, Wyatt RG, Kalica AR, Flores J, Hoshino Y (1983) Production and preliminary characterization of monoclonal antibodies directed at two surface proteins of rhesus rotavirus. J Virol 47: 267–275

    Google Scholar 

  19. Gunasena S, Nakagomi O, Isegawa Y, Kaga E, Nakagomi T, Steele AD, Flores J, Ueda S (1993) Relative frequency of VP4 gene alleles among human rotaviruses recovered over a 10-year period (1982–1991) from Japanese children with diarrhea. J Clin Microbiol 31: 2195–2197

    Google Scholar 

  20. Hardy ME, Gorziglia M, Woode GN (1993) The outer capsid protein VP4 of equine rotavirus strain H-2 represents a unique VP4 type by amino acid sequence analysis. Virology 193: 492–497

    Google Scholar 

  21. Hardy ME, Woode GN, Xu ZC, Williams JD, Conner ME, Dwyer RM, Powell DG (1991) Analysis of serotypes and electropherotypes of equine rotaviruses isolated in the United States. J Clin Microbiol 29: 889–893

    Google Scholar 

  22. Herring AJ, Inglis NF, Ojeh CK, Snodgrass DR, Menzies JD (1982) Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver-stained polyacrylamide gels. J Clin Microbiol 16: 473–477

    Google Scholar 

  23. Hoshino Y, Wyatt RG, Greenberg HB, Flores J, Kapikian AZ (1984) Serotypic similarity and diversity of rotaviruses of mammalian and avian origin as studied by plaque-reduction neutralization. J Infect Dis 149: 694–702

    Google Scholar 

  24. Hoshino Y, Wyatt RG, Greenberg HB, Kalica AR, Flores J, Kapikian AZ (1983) Isolation and characterization of an equine rotavirus. J Clin Microbiol 18: 585–591

    Google Scholar 

  25. Hoshino Y, Wyatt RG, Greenberg HB, Kalica AR, Flores J, Kapikian AZ (1983) Isolation, propagation, and characterization of a second equine rotavirus serotype. Infect Immun 41: 1031–1037

    Google Scholar 

  26. Hussein HA, Parwani AV, Rosen BI, Lucchelli A, Saif LJ (1983) Detection of rotavirus serotypes G1, G2, G3, and G11 in feces of diarrheic calves by using polymerase chain reaction-derived cDNA probes. J Clin Microbiol 31: 2491–2496

    Google Scholar 

  27. Iizuka M, Chiba M, Masamune O, Gerna G, Nakagomi O (1993) Molecular characterization of human rotavirus VP4 genes by polymerase chain reaction and restriction fragment length polymorphism assay. Microbiol Immunol 37: 729–735

    Google Scholar 

  28. Imagawa H, Tanaka T, Sekiguchi K, Fukunaga Y, Anzai T, Minamoto N, Kamada M (1993) Electropherotypes, serotypes, and subgroups of equine rotaviruses isolated in Japan. Arch Virol 131: 169–176

    Google Scholar 

  29. Imagawa H, Iizuka M, Fukunaga Y, Nakagomi O (1994) Molecular analysis of the VP7 genes of serotype G3 equine rotaviruses by polymerase chain reaction and restriction fragment length polymorphism assay. In: Nakajima H, Plowright W (eds) Equine infectious diseases. VII Proc Seventh Int Conf. R & W Publications, Newmarket, pp 79–83

    Google Scholar 

  30. Isa P, Snodgrass DR (1994) Serological and genomic characterization of equine rotavirus VP4 proteins identifies three different P serotypes. Virology 201: 364–372

    Google Scholar 

  31. Kaga E, Lizuka M, Nakagomi T, Nakagomi O (1994) The distribution of G (VP7) and P (VP4) serotypes among human rotaviruses recovered from Japanese children with diarrhea. Microbiol Immunol 38: 317–320

    Google Scholar 

  32. Li B, Gorziglia M (1993) VP4 serotype of the Gottfried strain of porcine rotavirus. J Clin Microbiol 31: 3075–3077

    Google Scholar 

  33. Li B, Larralde G, Gorziglia M (1993) Human rotavirus K8 strain represents a new VP4 serotype. J Virol 67: 617–620

    Google Scholar 

  34. Mackow ER, Shaw RD, Matsui SM, Vo PT, Benfield DA, Greenberg HB (1988) Characterization of homotypic and heterotypic VP7 neutralization sites of rhesus rotavirus. Virology 165: 511–517

    Google Scholar 

  35. Matsuda Y, Nakagomi O, Offit PA (1990) Presence of three P types (VP4 serotypes) and two G types (VP7 serotypes) among bovine rotavirus strains. Arch Virol 115: 199–207

    Google Scholar 

  36. Nagesha HS, Holmes IH (1991) Direct serotyping of porcine rotaviruses using VP7-specific monoclonal antibodies by an enzyme immunoassay. J Med Virol 35: 206–211

    Google Scholar 

  37. Nagesha HS, Holmes IH (1991) VP4 relationships between porcine and other rotavirus serotypes. Arch Virol 116: 107–118

    Google Scholar 

  38. Nakagomi O, Isegawa Y, Hoshino Y, Aboudy Y, Shif I, Silberstein I, Nakagomi T, Ueda S, Sears J, Flores J (1993) A new serotype of the outer capsid protein VP4 shared by an unusual human rotavirus strain Ro1845 and canine rotaviruses. J Gen Virol 74: 2771–2774

    Google Scholar 

  39. Nishikawa K, Hoshino Y, Taniguchi K, Green KY, Greenberg HB, Kapikian AZ, Chanock RM, Gorziglia M (1989) Rotavirus VP7 neutralization epitopes of serotype 3 strains. Virology 171: 503–515

    Google Scholar 

  40. Noel JS, Beards GM, Cubitt WD (1991) Epidemiological survey of human rotavirus serotypes and electropherotypes in young children admitted to two children's hospitals in northeast London from 1984 to 1990. J Clin Microbiol 29: 2213–2219

    Google Scholar 

  41. Ohta C, Hoshi A, Goto H, Tsunoda N, Tagami M, Akita H (1990) Epizootiological and virological studies of foal diarrhea associated with serotype 3 rotavirus. Jap J Vet Sci 52: 1049–1056

    Google Scholar 

  42. Parwani AV, Hussein HA, Rosen BI, Lucchelli A, Navarro L, Saif LJ (1983) Characterization of field strains of group A bovine rotaviruses by using polymerase chain reaction-generated G and P type-specific cDNA probes. J Clin Microbiol 31: 2010–2015

    Google Scholar 

  43. Pongsuwanna Y, Taniguchi K, Wakasugi F, Sutivijit Y, Chiwakul M, Warachit P, Jayavasu C, Urasawa S (1993) Distinct yearly change of serotype distribution of human rotavirus in Thailand as determined by ELISA and PCR. Epidemiol Infect 111: 407–412

    Google Scholar 

  44. Rassol NB, Green KY, Kapikian AZ (1993) Serotype analysis of rotaviruses from different locations in Malaysia. J Clin Microbiol 31: 1815–1819

    Google Scholar 

  45. Rasool NB, Larralde G, Gorziglia MI (1993) Determination of human rotavirus VP4 using serotype-specific cDNA probes. Arch Virol 133: 275–282

    Google Scholar 

  46. Redmond DL, Inglis NF, Fitzgerald TA, Snodgrass DR, Herring AJ (1992) A liquidhybridization method for typing the Vp4 and Vp7 genes of bovine rotaviruses. J Virol Methods 39: 165–177

    Google Scholar 

  47. Rosen BI, Parwani AV, Lopez S, Flores J, Saif LJ (1994) Serotypic differentiation of rotaviruses in field samples from diarrheic pigs by using nucleic acid probes specific for porcine VP4 and human and porcine VP7 genes. J Clin Microbiol 32: 311–317

    Google Scholar 

  48. Sereno MM, Gorziglia MI (1994) The outer capsid protein VP4 of murine rotavirus strain Eb represents a tentative new P type. Virology 199: 500–504

    Google Scholar 

  49. Shaw RD, Vo PT, Offit PA, Coulson BS, Greenberg HB (1986) Antigenic mapping of the surface proteins of rhesus rotavirus. Virology 155: 434–451

    Google Scholar 

  50. Snodgrass DR, Fitzgerald T, Campbell I, Scott FM, Browning GF, Miller DL, Herring AJ, Greenberg HB (1990) Rotavirus serotypes 6 and 10 predominate in cattle. J Clin Microbiol 28: 504–507

    Google Scholar 

  51. Snodgrass DR, Hoshino Y, Fitzgerald TA, Smith M, Browning GF, Gorziglia M (1992) Identification of four VP4 serological types (P serotypes) of bovine rotavirus using viral reassortants. J Gen Virol 73: 2319–2325

    Google Scholar 

  52. Suzuki Y, Sanekata T, Sato M, Tajima K, Matsuda Y, Nakagomi O (1993) Relative frequencies of G (VP7) and P (VP4) serotypes determined by polymerase chain reaction assays among Japanese bovine rotaviruses isolated in cell culture. J Clin Microbiol 31: 3046–3049

    Google Scholar 

  53. Takagi M, Taniguchi K, Urasawa T, Urasawa S, Shirahata T, Goto H (1994) Characterization of a G14 equine rotavirus (strain CH3) isolated in Japan. Arch Virol 139: 209–215

    Google Scholar 

  54. Taniguchi K, Urasawa T, Urasawa S (1994) Species specificity and interspecies relatedness in VP4 genotypes demonstrated by VP4 sequence analysis of equine, feline, and canine rotavirus strains. Virology 200: 390–400

    Google Scholar 

  55. Ushijima H, Mukoyama A, Hasegawa A, Nishimura S, Konishi K, Bosu K (1994) Serotyping of human rotaviruses in the Tokyo area (1990–1993) by enzyme immunoassay with monoclonal antibodies and by reverse transcription and polymerase chain reaction amplification. J Med Virol 44: 162–165

    Google Scholar 

  56. Woods PA, Gentsch J, Gouvea V, Mata L, Santosham M, Bai ZS, Urasawa S, Glass RI (1992) Distribution of serotypes of human rotavirus in different populations. J Clin Microbiol 30: 781–785

    Google Scholar 

  57. Wu H, Taniguchi K, Urasawa T, Urasawa S (1993) Genomic relatedness of five equine rotavirus strains with different G serotype and P type specificities. Res Virol 144: 455–464

    Google Scholar 

  58. Wu H, Taniguchi K, Wakasugi F, Ukae S, Chiba S, Ohseto M, Hasegawa A, Urasawa T, Urasawa S (1994) Survey on the distribution of the gene 4 alleles of human rotaviruses by polymerase chain reaction. Epidemiol Infect 112: 615–622

    Google Scholar 

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  1. Veterinary Preclinical Centre, Faculty of Veterinary Science, The University of Melbourne, Parkville, Victoria

    G. F. Browning

  2. Scone Veterinary Diagnostic Laboratory, Scone, New South Wales, Australia

    A. P. Begg

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Browning, G.F., Begg, A.P. Prevalence of G and P serotypes among equine rotaviruses in the faeces of diarrhoeic foals. Archives of Virology 141, 1077–1089 (1996). https://doi.org/10.1007/BF01718611

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