Summary
Pseudorabies virus (PRV) early protein 0 (EP0) is a transactivator containing the RING finger domain. Analysis of transactivating activity of truncated forms of the EP0 molecule consisting of 410 amino acids revealed that amino-terminal region containing the RING finger domain, amino acids 1 to 84, and the region between amino acids 114 to 242 containing acidic amino acid sequences were required for the transactivation. On the other hand, the mutant consisting of amino acids 1 to 113 exhibited a dominant-negative property.
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References
Cai W, Schaffer PA (1989) Herpes simplex virus type 1 ICP0 plays a critical role in the de novo synthesis of infectious virus following transfection of viral DNA. J Virol 63: 4579–4589
Chen J, Zhu X, Silverstein S (1991) Mutational analysis of the sequence encoding ICP0 from herpes simplex virus type 1. Virology 180: 207–220
Cheung AK (1991) Cloning of the latency gene and the early protein 0 gene of pseudorabies virus. J Virol 65: 5260–5271
Everett RD (1984)Trans activation of transcription by herpes virus products: requirement for two HSV-1 immediate-early polypeptides for maximum activity. EMBO J 3: 3135–3141
Everett RD (1987) A detailed mutational analysis of Vmw 110, atrans-acting transcriptional activator encoded by herpes simplex virus type 1. EMBO J 6: 2069–2076
Everett RD (1988) Analysis of the functional domains of herpes simplex virus type 1 immediate-early polypeptide Vmw 110. J Mol Biol 202: 87–96
Everett RD, Barlow P, Milner A, Luisi B, Orr A, Hope G, Lyon D (1993) A novel arrangement of zinc-binding residues and secondary structure in the C3HC4 motif of an alpha herpes virus protein family. J Mol Biol 234: 1038–1047
Everett RD, Cross A, Orr A (1993) A truncated form of herpes simplex virus type 1 immediate-early protein Vmw 110 is expressed in a cell type dependent manner. Virology 197: 751–756
Fraefel C, Zeng J, Choffat Y, Engels M, Schwyzer M, Ackermann M (1994) Identification and zinc dependence of the bovine herpesvirus 1 transactivator protein BICP0. J Virol 68: 3154–3162
Freemont PS (1993) The RING finger. A novel protein sequence motif related to the zinc finger. Ann N Y Acad Sci 684: 174–192
Kalderon D, Richardson WD, Markham AF, Smith AE (1984) Sequence requirements for nuclear location of simian virus 40 large-T antigen. Nature 311: 33–38
Laemmli UK (1970) Cleavage of structural proteins during the assembly to the head of bacteriophage T4. Nature 227: 680–685
Mitchell PJ, Tjian R (1989) Transcriptional regulation in mammalian cells by sequence-specific DNA binding proteins. Science 245: 371–378
Moriuchi H, Moriuchi M, Cohen JI (1994) The RING finger domain of the varicellazoster virus open reading frame 61 protein is required for its transregulatory functions. Virology 205: 238–246
Moriuchi H, Moriuchi M, Dean H, Cheung AK, Cohen JI (1995) Pseudorabies virus EP0 is functionally homologous to varicella-zoster virus ORF61 protein and herpes simplex virus type 1 ICP0. Virology 209: 281–283
Moriuchi H, Moriuchi M, Smith HA, Straus SE, Cohen JI (1992) Varicella-zoster virus open reading frame 61 protein is functionally homologous to herpes simplex virus type 1 ICP0. J Virol 66: 7303–7308
Moriuchi H, Moriuchi M, Straus SE, Cohen JI (1993) Varicella-zoster virus (VZV) open reading frame 61 protein transactivates VZV gene promoters and enhances the infectivity of VZV DNA. J Virol 67: 4290–4295
Nagpal S, Ostrove JM (1991) Characterization of a potent varicella-zoster virus-encodedtrans-repressor. J Virol 65: 5289–5296
O'Hare P, Hayward GS (1985) Three trans-acting regulatory proteins of herpes simplex virus modulate immediate-early gene expression in a pathway involving positive and negative feedback regulation. J Virol 56: 723–733
Peeples ME (1988) Differential detergent treatment allows immunofluorescent localization of the Newcastle disease virus matrix protein within the nucleus of infected cells. Virology 162: 255–259
Taharaguchi S, Inoue H, Ono E, Kida H, Yamada S, Shimizu Y (1994) Mapping of transcriptional regulatory domains of pseudorabies virus immediate-early protein. Arch Virol 137: 289–302
Towbin H, Staehelin T, Gordon T (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76: 4350–4354
Viček Č, Kozmík Z, Pačes V, Schirm S, Schwyzer M (1990) Pseudorabies virus immediate-early gene overlaps with an oppositely oriented open reading frame: characterization of their promoter and enhancer regions. Virology 179: 365–377
Watanabe S, Ono E, Shimizu Y, Kida H (1995) Pseudorabies virus early protein 0 transactivates the viral gene promoters. J Gen Virol 76: 2881–2885
Weber PC, Kenny JJ, Wigdahl B (1992) Antiviral properties of a dominant negative mutant of herpes simplex virus type 1 regulatory protein ICP0. J Gen Virol 73: 2955–2961
Weber PC, Wigdahl B (1992) Identification of dominant-negative mutants of the herpes simplex virus type 1 immediate-early protein ICP0. J Virol 66: 2261–2267
Wirth UV, Fraefel C, Vogt B, Vlček Č, Pačes V, Schwyzer M (1992) Immediate-early RNA 2.9 and early RNA 2.6 of bovine herpesvirus 1 and 3′ coterminal and encode a putative zinc finger transactivator protein. J Virol 66: 2763–2772
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Watanabe, S., Ono, E., Shimizu, Y. et al. Mapping of transregulatory domains of pseudorabies virus early protein 0 and identification of its dominant-negative mutant. Archives of Virology 141, 1001–1009 (1996). https://doi.org/10.1007/BF01718604
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DOI: https://doi.org/10.1007/BF01718604