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Molecular cloning and nucleotide sequencing of the 3′-terminal region of a South African isolate of ryegrass mosaic virus RNA and in vitro expression of the coat protein gene

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Summary

The 2094 nucleotides at the 3′-terminus of a South African isolate of ryegrass mosaic virus (RGMV) was cloned and sequenced. Two putative poly-protein cleavage sites were found: Q/L and E/A, both of which are novel in thePotyviridae. The RGMV-SA cDNA was cloned into an expression vector, pUEX, and a fusion protein of 185 kDa was obtained which reacted strongly to anti-RGMV-SA antiserum. Alignment of the predicted amino acid sequence of RGMV-SA with those of otherPotyviridae members showed limited identity, indicating that RGMV-SA is a definite and distinct virus.

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Authors and Affiliations

  1. Range and Forage Institute, Pretoria

    S. N. Salm

  2. Department of Microbiology, University of the Witwatersrand, Johannesburg, South Africa

    M. E. C. Rey

  3. Department of Plant Pathology, USDA-ARS, University of Nebraska, Lincoln, Nebraska, USA

    R. French

Authors
  1. S. N. Salm
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  2. M. E. C. Rey
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  3. R. French
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Salm, S.N., Rey, M.E.C. & French, R. Molecular cloning and nucleotide sequencing of the 3′-terminal region of a South African isolate of ryegrass mosaic virus RNA and in vitro expression of the coat protein gene. Archives of Virology 141, 185–195 (1996). https://doi.org/10.1007/BF01718600

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  • DOI: https://doi.org/10.1007/BF01718600

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