Skip to main content
SpringerLink
Log in

Sequence analysis of the 5′ ends of tomato spotted wilt virus N mRNAs

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

Messenger RNAs transcribed from the tomato spotted wilt virus (TSWV) RNA genome have characteristic extra non-templated heterogeneous sequences at their 5′ ends which may be the result of a cap-snatching event involving cellular mRNAs. In order to investigate the genetic origin of these extra sequences and to gain more insight in the process of cap-snatching as performed by TSWV, nucleocapsid protein (N) mRNAs derived from the TSWV S RNA were cloned and sequenced. Twenty clones were obtained which contained 5′-proximal sequences of non-viral origin, ranging in length from 12 to 21 nucleotides. None of the sequences analyzed were identical and no base preference at the endonucleolytic site was observed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Beaton AR, Krug RM (1981) Selected host cell capped RNA fragments prime influenza viral RNA transcription in vivo. Nucleic Acids Res 9: 4423–4436

    Google Scholar 

  2. Bishop DHL, Gay ME, Matsuoko Y (1983) Nonviral heterogeneous sequences are present at the 5′ ends of one species of snowshoe hare bunyavirus S complementary RNA. Nucleic Acids Res 11: 6409–6418

    Google Scholar 

  3. Bouloy M, Hannoun C (1976) Studies on Lumbo virus replication. I. RNA dependent RNA polymerase associated with virions. Virology 69: 258–268

    Google Scholar 

  4. Bouloy M, Plotch SJ, Krug RM (1978) Globin mRNAs are primers for the transcription of influenza viral RNA in vitro. Proc Natl Acad Sci USA 75: 486–4890

    Google Scholar 

  5. Bouloy M, Pardignon N, Vialat P, Gerbaud S, Girard M (1990) Characterization of the 5′ and 3′ ends of viral mRNAs isolated from BHK21 cells infected with Germiston virus (Bunyavirus). Virology 175: 50–58

    Google Scholar 

  6. Collett MS (1986) Messenger RNA of the M segment RNA of Rift Valley fever virus. Virology 151: 151–156

    Google Scholar 

  7. De Haan P, Wagemakers L, Peters D, Goldbach R (1989) Molecular cloning and terminal sequence determination of the S and M RNAs of tomato spotted wilt virus. J Gen Virol 70: 3469–3473

    Google Scholar 

  8. De Haan P, Wagemakers L, Peters D, Goldbach R (1990) The S RNA segment of tomato spotted wilt has an ambisense character. J Gen Virol 71: 1001–1007

    Google Scholar 

  9. De Haan P, Kormelink R, Resende R de O, Van Poelwijk F, Peters D, Goldbach R (1991) Tomato spotted wilt virus LRNA encodes a putative RNA polymerase. J Gen Virol 72: 2207–2216

    Google Scholar 

  10. Dumas JB, Edwards M, Delort J, Mallet J (1991) Oligodeoxyribonucleotide ligation to single-stranded cDNAs: a new tool for cloning 5′ ends of mRNAs and for constructing cDNA libraries byin vitro amplification. Nucleic Acids Res 19: 5227–5232

    Google Scholar 

  11. Eshita Y, Ericson B, Romanowski V, Bishop DHL (1985) Analyses of the mRNA transcription processes of snowshoe hare bunyavirus S and M RNA species. J Virol 55: 681–689

    Google Scholar 

  12. Francki RIB, Fauquet CM, Knudson DL, Brown F (1991) Classification and Nomenclature of Viruses. Fifth Report of the International Committee on Taxonomy of Viruses. Springer Wien New York (Arch Virol [Suppl] 2)

    Google Scholar 

  13. Garcin D, Kolakofsky D (1990) A novel mechanism for the initiation of Tacaribe arenavirus genome replication. J Virol 64: 6196–6203

    Google Scholar 

  14. Gerbaud S, Pardigon N, Vialat P, Bouloy M (1987) The S segment of the Germiston Bunyavirus genome: coding strategy and transcription. In: Mahy B, Kolakofsky D (eds) The biology of negative strand viruses. Elsevier, Amsterdam, pp 191–198

    Google Scholar 

  15. Huiet L, Feldstein PA, Tasi JH, Falk BW (1993) The maize stripe virus major noncapsid protein messenger RNA transcripts contain heterogeneous leader sequences at their 5′ termini. Virology 197: 808–812

    Google Scholar 

  16. Ihara T, Matsuura Y, Bishop DHL (1985) Analyses of the mRNA transcription process of Punta Toro phlebovirus (Bunyaviridae). Virology 147: 317–325

    Google Scholar 

  17. Jin H, Elliott RM (1991) Expression of functional Bunyamwera virus L protein by recombinant vaccinia virus. J Virol 65: 4182–4189

    Google Scholar 

  18. Jin H, Elliott RM (1992) Mutagenesis of the L protein encoded by Bunyamwera virus and production of monospecific antibodies. J Gen Virol 73: 2235–2244

    Google Scholar 

  19. Jin H, Elliott RM (1993a) Characterization of Bunyamwera virus S RNA that is transcribed and replicated by the L protein expressed from recombinant vaccinia virus. J Virol 67: 1396–1404

    Google Scholar 

  20. Jin H, Elliott RM (1993b) Non-viral sequences at the 5′ ends of Dugbe nairovirus S mRNAs. J Gen Virol 74: 2293–2297

    Google Scholar 

  21. Kormelink R, Kitajima EW, De Haan P, Zuidema D, Peters D, Goldbach R (1991) The nonstructural protein (NSs) encoded by the ambisense S RNA segment of tomato spotted wilt virus is associated with fibrous structures in infected plant cells. Virology 181: 459–468

    Google Scholar 

  22. Kormelink R, Van Poelwijk F, Peters D, Goldbach R (1992a) Non-viral heterogeneous sequences at the 5′ ends of tomato spotted wilt virus mRNAs. J Gen Virol 73: 2125–2128

    Google Scholar 

  23. Kormelink R, De Haan P, Meurs C, Peters D, Goldbach (1992b) The nucleotide sequence of the M RNA segment of tomato spotted wilt virus: a bunyavirus with two ambisense RNA segments. J Gen Virol 73: 2795–2804

    Google Scholar 

  24. Krug RM (1981) Priming of influenza viral RNA transcription by capped heterologous RNAs. Curr Top Microbiol Immunol 93: 125–149

    Google Scholar 

  25. Lamb RA, Lai C-J, Choppin PW (1981) Sequences of mRNAs derived from genome RNA segment 7 of influenza virus: colinear and interrupted mRNAs code for overlapping proteins. Proc Natl Acad Sci USA 78: 4170–4174

    Google Scholar 

  26. Patterson JL, Kolakofsky D (1984) Characterization of La Crosse virus small-genome transcripts. J Virol 29: 680–685

    Google Scholar 

  27. Plotch SJ, Bouloy M, Ulmanen I, Krug RM (1981) A unique cap (m7GpppXm)-dependent influenza virion endonuclease cleaves capped RNAs to generate the primers that initiate viral RNA transcription. Cell 23: 847–858

    Google Scholar 

  28. Raju R, Raju L, Hacker D, Garcin D, Compans R, Kolakofsky D (1990) Nontemplated bases of the 5′ ends of Tacaribe virus mRNAs. Virology 174: 53–59

    Google Scholar 

  29. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, New York

    Google Scholar 

  30. Shaw MW, Lamb RA (1984) A specific sub-set of host-cell mRNAs prime influenza virus mRNA synthesis. Virus Res 1: 455–467

    Google Scholar 

  31. Simons JF, Petterson RF (1991) Host-derived 5′ ends and overlapping complementary 3′ ends of the two mRNAs transcribed from the ambisense S segment of Uukuniemi virus. J Virol 65: 4741–4748

    Google Scholar 

  32. Van Poelwijk F, Boye K, Oosterling R, Peters D, Goldbach R (1993) Detection of the L protein of tomato spotted wilt virus. Virology 197: 468–470

    Google Scholar 

  33. Vialat P, Bouloy M (1992) Germiston virus transcriptase requires active 40S ribosomal subunits and utilizes capped cellular RNAs. J Virol 66: 685–693

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Virology, Wageningen Agricultural University, Wageningen, The Netherlands

    F. van Poelwijk, J. Kolkman & R. Goldbach

Authors
  1. F. van Poelwijk
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Kolkman
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. Goldbach
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

van Poelwijk, F., Kolkman, J. & Goldbach, R. Sequence analysis of the 5′ ends of tomato spotted wilt virus N mRNAs. Archives of Virology 141, 177–184 (1996). https://doi.org/10.1007/BF01718599

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01718599

Keywords

Search

Navigation