Skip to main content
SpringerLink
Log in

Identification of a unique VP4 serotype that is shared by a human rotavirus (69M strain) and an equine rotavirus (H-2 strain)

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

A cDNA clone representing the VP4-encoding gene of human rotavirus strain 69M(VP7 serotype 8) was constructed and inserted into a baculovirus expression vector. Baculovirus recombinants that expressed the 69 M VP4 protein inSpodoptera frugiperda (Sf9) cells were screened by immunofluorescence with hyperimmune antiserum to the 69M strain and purified by terminal dilution. The expressed VP4 was detected by Coomassie blue staining of PAGE-separated proteins. The antigenic relationships between the VP4 of the 69M strain and those of various human and other animal rotavirus strains representing ten established VP4 serotypes were examined by plaque reduction neutralization. Hyperimmune antiserum produced in guinea pigs following immunization with a lysate of Sf9 cells infected with a 69M gene 4-baculovirus recombinant neutralized the infectivity of the homologous human rotavirus 69M strain as well as heterologous equine rotavirus H-2 strain to a high titer. The anti-69M VP4 hyperimmune antiserum did not neutralize significantly other rotavirus strains of human, simian, porcine, bovine, or murine origin. It thus appears that the human rotavirus 69M strain has a distinct VP4 serotype (designated as P serotype 4) which is closely related antigenically to equine rotavirus H-2 VP4.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Albert MJ, Unicomb LE, Bishop RF (1987) Cultivation and characterization of human rotaviruses with “super short” RNA patterns. J Clin Microbiol 25: 183–185

    Google Scholar 

  2. Estes MK, Cohen J (1989) Rotavirus gene structure and function. Microbiol Rev 53: 410–449

    Google Scholar 

  3. Gorziglia M, Hoshino Y, Buckler-White A, Blumentals I, Glass R, Flores J, Kapikian AZ, Chanock RM (1986) Conservation of amino acid sequence of VP8 and cleavage region of 84-kDa outer capsid protein among rotaviruses recovered from asymptomatic neonatal infection. Proc Natl Acad Sci USA 83: 7039–7043

    Google Scholar 

  4. Gorziglia M, Green K, Nishikawa N, Taniguchi K, Jones R, Kapikian AZ, Chanock RM (1988) Sequence of fourth gene of human rotaviruses recovered from asymptomatic or symptomatic infections. J Virol 62: 2978–2984

    Google Scholar 

  5. Gorziglia M, Larralde G, Kapikian AZ, Chanock RM (1990) Antigenic relationships among human rotaviruses as determined by outer capsid VP4. Proc Natl Acad Sci USA 87: 7155–7159

    Google Scholar 

  6. Gouvea V, DeCastro L, Timenetsky MDC, Greenberg H, Santos N (1994) Rotavirus serotype G5 associated with diarrhea in Brazilian children. J Clin Microbiol 32: 1408–1409

    Google Scholar 

  7. Hardy ME, Gorziglia M, Woode GN (1993) The outer capsid protein VP4 of equine rotavirus H-2 represents a unique VP4 type by amino acid sequence analysis. Virology 193: 492–497

    Google Scholar 

  8. Hasegawa A, Inouye S, Matsuno S, Yamaoka E, Eko R, Suharyono W (1984) Isolation of human rotavirus with a distinct RNA electrophoretic pattern from Indonesia. Microbiol Immunol 28: 719–722

    Google Scholar 

  9. Hoshino Y, Wyatt RG, Greenberg HB, Kalica AR, Flores J, Kapikian AZ (1983) Isolation and characterization of an equine rotavirus. J Clin Microbiol 18: 585–591

    Google Scholar 

  10. Hoshino Y, Saif LJ, Sereno MM, Chanock RM, Kapikian AZ (1988) Infection immunity of piglets to either VP3 or VP7 outer capsid protein confers resistance to challenge with a virulent rotavirus bearing the corresponding antigen. J Virol 62: 744–748

    Google Scholar 

  11. Hoshino Y, Kapikian AZ (1994) Rotavirus antigens. Curr Top Microbiol Immunol 185: 179–227

    Google Scholar 

  12. Isa P, Snodgrass DR (1994) Serological and genomic characterization of equine rotavirus. VP4 proteins identifies three different P serotypes. Virology 201: 364–372

    Google Scholar 

  13. Kapikian AZ, Chanock RM (1990) Rotaviruses. In: Fields BN, Knipe DM, Chanock RM, Hirsch MS, Melnick JL, Monath TP, Roizman B (eds) Virology, 2nd ed. Raven Press, New York, pp 1353–1404

    Google Scholar 

  14. Matsui SM, Mackow ER, Greenberg HB (1989) Molecular determinant of rotavirus neutralization and protection. Adv Virus Res 36: 181–214

    Google Scholar 

  15. Matsuno S, Hasegawa A, Mukoyama A, Inouye S (1985) A candidate for a new serotype of human rotavirus. J Virol 54: 623–624

    Google Scholar 

  16. Nishikawa K, Fukuhara N, Liprandi F, Green K, Kapikian AZ, Chanock RM, Gorziglia M (1989) VP4 protein of porcine rotavirus strain OSU expressed by a baculovirus recombinant induces neutralizing antibodies. Virology 173: 631–637

    Google Scholar 

  17. Offit PA, Shaw RD, Greenberg HB (1986) Passive protection against rotavirus-induced diarrhea by monoclonal antibodies to surface proteins VP3 and VP7. J Virol 58: 700–703

    Google Scholar 

  18. Prasad BVV, Wang GJ, Clerx JPM, Chiu W (1988) Three-dimensional structure of rotavirus. J Mol Biol 199: 269–275

    Google Scholar 

  19. Qian Y, Green KY (1991) Human rotavirus strain 69M has a unique VP4 as determined by amino acid sequence analysis. Virology 182: 407–412

    Google Scholar 

  20. Sereno MM, Gorziglia M (1994) The outer capsid protein VP4 of murine rotavirus strain Eb represents a tentative new P type. Virology 199: 500–504

    Google Scholar 

  21. Snodgrass DR, Hoshino Y, Fitzgerald TA, Smith M, Browning GF, Gorziglia M (1992) Identification of four VP4 serological types (P serotypes) of bovine rotavirus using viral ressortants. J Gen Virol 73: 2319–2325

    Google Scholar 

Download references

Author information

Author notes

  1. B. Li

    Present address: Division of Molecular Biological Research and Evaluation, Food and Drug Administration, Washington, DC

  2. M. Gorziglia

    Present address: Genetic Therapy, Inc., Gaithersburg, MD, USA

Authors and Affiliations

  1. Epidemiology Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA

    B. Li, Y. Hoshino & M. Gorziglia

Authors
  1. B. Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Y. Hoshino
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Gorziglia
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Li, B., Hoshino, Y. & Gorziglia, M. Identification of a unique VP4 serotype that is shared by a human rotavirus (69M strain) and an equine rotavirus (H-2 strain). Archives of Virology 141, 155–160 (1996). https://doi.org/10.1007/BF01718596

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01718596

Keywords

Search

Navigation