Summary
Large-scale surveys in Africa for blackeye cowpea mosaic (BlCMV) and cowpea aphid-borne mosaic (CABMV) showed that several CABMV isolates from Southern Africa were either not or poorly recognized by monoclonal antibodies prepared to isolates collected in West Africa. Selection of three new monoclonal antibodies prepared against the Maputo (Mozambique) isolate of CABMV, and their incorporation into a revised panel of monoclonal antibodies, resulted in the assignment of four of these new CABMV isolates to existing serotypes (II, IV, and V) and three others to a new serotype (VI). The South African isolate of passiflora mosaic virus was shown to be related to CABMV isolates in serotype IV. It is proposed that CABMV isolates be assembled into a distinct species in the legume-infecting, aphid-transmissible potyviruses.
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References
Bashir M, Hampton RO (1991a) Blackeye cowpea mosaic (BlCMV) and cowpea aphid-borne mosaic (CAMV) potyviruses: biological comparisons and serological distinctions. Phytopathology 81: 1166 (Abstr.)
Bashir M, Hampton RO (1991b) Viruses seedborne in cowpea: significance for cowpea breeding programs and production. Hort Sci 26: 493 (Abstr.)
Brand RJ (1992) Viruses implicated in the woodiness disease of South African passionfruit, and the molecular characterization of a new potyvirus. PhD thesis, University of Cape Town
Brand RJ, Burger JT, Rybicki EP (1993) Cloning, sequencing, and expression inEscherichia coli of the coat protein gene of a new potyvirus infecting South AfricanPassiflora. Arch Virol 128: 29–41
Fischer HU, Lockhart BE (1976) A strain of cowpea aphid-borne mosaic virus isolated from cowpeas in Morocco. Phytopathol Z 85: 43–48
Huguenot C, Furneaux MT, Thottappilly G, Rossel HW, Hamilton RI (1993) Evidence that cowpea aphid-borne mosaic and blackeye cowpea mosaic viruses are two different potyviruses. J Gen Virol 74: 335–340
Huguenot C, Furneaux MT, Hamilton RI (1994) Capsid protein properties of cowpea aphid-borne mosaic virus and blackeye cowpea mosaic virus confirm the existence of subgroups of aphid-transmitted, legume-infecting potyviruses. J Gen Virol 75: 3555–3560
Konate G, Neya J (1995) Rapid detection of cowpea aphid-borne mosaic virus in cowpea seeds. Phytoprotection 75 (in press)
Lovisolo O, Conti M (1966) Identification of an aphid-transmitted cowpea mosaic virus. Neth J Plant Pathol 72: 265–269
Mali VR, Kulthe KS (1980) A seedborne potyvirus causing mosaic of cowpea in India. Plant Dis 64: 925–928
Mali VR, Munde GE, Patil NS, Kulthe KS (1988) Detection and identification of blackeye cowpea mosaic and cowpea aphid-borne mosaic viruses in India. Int J Trop Plant Dis 6: 159–173
McKern NM, Mink GI, Barnett OW, Mishra A, Whittaker LA, Silbernagel MJ, Ward CW, Shukla DD (1992) Isolates of bean common mosaic virus comprising two distinct serotypes. Phytopathology 82: 923–929
McKern NM, Strike PM, Barnett OW, Dijkstra J, Shukla DD, Ward CW (1994) Cowpea aphid-borne mosaic virus Morocco and South African passiflora virus are strains of the same potyvirus. Arch Virol 136: 207–217
Mink GI, Vetten HJ, Ward CW, Berger PH, Morales FJ, Myers JM, Silbernagel MJ, Barnett OW (1994) Taxonomy and classification of legume-infecting potyviruses. Arch Virol 139: 231–235
Pietersen G, Garnett HM (1990) A survey for the viruses of soybeans (Glycine max) in the Transvaal, South Africa. Phytophylactica 22: 35–40
Provvidenti R, Gonsalves D, Taiwo MA (1993) Inheritance of resistance of blackeye cowpea mosaic and cowpea aphid-borne mosaic virus inPhaseolus vulgaris. J Heredity 74: 60–61
Taiwo MA, Gonsalves D, Provvidenti R, Thurston HD (1982) Partial characterization and grouping of isolates of blackeye cowpea mosaic and cowpea aphid-borne mosaic viruses. Phytopathology 72: 590–596
Thottappilly G, Hamilton RI, Huguenot C, Rossel HW, Furneaux MT, Gumedzoe MY, Shoyinka SA, Naik DM, Konate G, Atcham-Agneroh T, Haciwa HC, Anno-Nyako FO, Saifodine N, Wangai A, Lamptey P, Gubba A, Mbwaga AM, Neya J, Offei SK (1993) Identification of cowpea viruses and strains in tropical Africa — an International pilot project. FAO Plant Prot Bull 41: 65–72
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Huguenot, C., Furneaux, M.T., Clare, J.A. et al. Improved diagnosis of cowpea aphid-borne mosaic virus in Africa: significance for cowpea seed-indexing, breeding programs and potyvirus taxonomy. Archives of Virology 141, 137–145 (1996). https://doi.org/10.1007/BF01718594
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DOI: https://doi.org/10.1007/BF01718594