Skip to main content

Advertisement

SpringerLink
Log in

Genetic complementation between replication-defective mutants of HIV-1 and SIVagm

  • Original Papers
  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

To investigate the functional complementation of essential genes for virus growth between HIV-1 and SIVagm derived from African green monkeys, we co-transfected replication-defective molecular clones containing mutations ingag, pol, env, tat orrev, and monitored transient complementation by reverse transcriptase assay (RT), cytopathic effect (CPE) and immunofluorescence assay (IFA). The following results were obtained: 1) No complementation was observed in combinations of thegag andpol mutants. 2) Therev mutant of HIV-1 was minimally complemented by other SIVagm mutants, although therev mutant of SIVagm was significantly complemented by other HIV-1 mutants. 3) Among all combinations tested, theenv mutant of HIV-1 was the most effectively complemented by SIVagm mutants. 4) CPE was mostly absent in combinations of theenv mutant of SIVagm and thegag, pol ortat mutants of HIV-1, although there were significant positive results in RT and IFA assays. These findings provided basic information about the functional compatibility of pathogenic HIV-1 and nonpathogenic SIVagm which will be useful for generating chimeras of these two viruses.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Adachi A, Gendelman HE, Koenig S, Folks T, Willey R, Rabson A, Martin MA (1986) Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone. J Virol 59: 284–291

    Google Scholar 

  2. Adachi A, Ono N, Sakai H, Ogawa K, Shibata R, Kiyomasu T, Masuike H, Ueda S (1991) Generation and characterization of the human immunodeficiency virus type 1 mutants. Arch Virol 117: 45–58

    Google Scholar 

  3. Arya SK, Gallo RC (1988) Human immunodeficiency virus type 2 long terminal repeat: analysis of regulatory elements. Proc Natl Acad Sci USA 85: 9753–9757

    Google Scholar 

  4. Chakrabarti L, Guyader M, Alizon M, Daniel MD, Desrosiers RC, Tiollais P, Sonigo P (1987) Sequence of simian immunodeficiency virus from macaque and its relationship to other human and simian retroviruses. Nature 328: 543–547

    Google Scholar 

  5. de Mareuil J, Brichacek B, Salaun D, Chermann JC, Hirsch I (1992) The human immunodeficiency virus (HIV)gag gene product p18 is responsible for enhanced fusogenicity and host range tropism of the highly cytopathic HIV-1-NDK strain. J Virol 66: 6797–6801

    Google Scholar 

  6. Emerman M, Guyader M, Montagnier L, Baltimore D, Muesing MA (1987) The specificity of the human immunodeficiency virus type 2 transactivator is different from that of human immunodeficiency virus type 1. EMBO J 6: 3755–3760

    Google Scholar 

  7. Ennen J, Findeklee H, Dittmar MT, Norley S, Ernst M, Kurth R (1994) CD8+ T lymphocytes of African green monkey secrete an immunodeficiency virus-suppressing lymphokine. Proc Natl Acad Sci USA 91: 7207–7211

    Google Scholar 

  8. Franchini G, Gurgo C, Guo HG, Gallo RC, Collalti E, Fargnoli KA, Hall LF, Wong SF, Reitz MJ (1987) Sequence of simian immunodeficiency virus and its relationship to the human immunodeficiency viruses. Nature 328: 539–543

    Google Scholar 

  9. Fukasawa M, Miura T, Hasegawa A, Morikawa S, Tsujimoto H, Miki K, Kitamura T, Hayami M (1988) Sequence of simian immunodeficiency virus from African green monkey, a new member of the HIV/SIV group. Nature 333: 457–461

    Google Scholar 

  10. Fukui M, Yamamoto T, Kawai S, Maruo K, Toyoshima K (1985) Detection of a raf-related and two other transforming DNA sequences in human tumors maintained in nude mice. Proc Natl Acad Sci USA 82: 5954–5958

    Google Scholar 

  11. Gluzman Y (1981) SV40-transformed simian cells support the replication of early SV40 mutants. Cell 23: 175–182

    Google Scholar 

  12. Guyader M, Emerman M, Sonigo P, Clavel F, Montagnier L, Alizon M (1987) Genome organization and transactivation of the human immunodeficiency virus type 2. Nature 326: 662–669

    Google Scholar 

  13. Hasegawa A, Tsujimoto H, Maki N, Ishikawa K, Miura T, Fukasawa M, Miki K, Hayami M (1989) Genomic divergence of HIV-2 from Ghana. AIDS Res Hum Retroviruses 5: 593–604

    Google Scholar 

  14. Hirsch VM, Dapolito G, Johnson PR, Elkins WR, London WT, Montali RJ, Goldstein S, Brown C (1995) Induction of AIDS by simian immunodeficiency virus from an African green monkey: species-specific variation in pathogenicity correlates with the extent of in vivo replication. J Virol 69: 955–967

    Google Scholar 

  15. Hirsch VM, Dapolito GA, Goldstein S, McClure H, Emau P, Fultz PN, Isahakia M, Lenroot R, Myers G, Johnson PR (1993) A distinct African lentivirus from Sykes' monkeys. J Virol 67: 1517–1528

    Google Scholar 

  16. Hirsch VM, Olmsted RA, Murphey CM, Purcell RH, Johnson PR (1989) An African primate lentivirus (SIVsm) closely related to HIV-2. Nature 339: 389–392

    Google Scholar 

  17. Hoshikawa N, Kojima A, Yasuda A, Takayashiki E, Masuko S, Chiba J, Sata T, Kurata T (1991) Role of thegag andpol genes of human immunodeficiency virus in the morphogenesis and maturation of retrovirus-like particles expressed by recombinant vaccinia virus: an ultrastructural study. J Gen Virol 72: 2509–2517

    Google Scholar 

  18. Huet T, Cheynier R, Meyerhans A, Roelants G, Wain HS (1990) Genetic organization of a chimpanzee lentivirus related to HIV-1 [see comments]. Nature 345: 356–359

    Google Scholar 

  19. Igarashi T, Shibata R, Hasebe F, Ami Y, Shinohara K, Komatu T, Stahl-Hening C, Petry H, Hunsmann G, Kuwata T, Jin M, Adachi A, Kurimura T, Okada M, Miura T, Hayami M (1994) Persistent infection with SIVmac chimeric virus havingtat, rev, vpu, env andnef of HIV type 1 in macaque monkeys. AIDS Res Hum Retroviruses 10: 1021–1029

    Google Scholar 

  20. Jacks T, Power MD, Masiarz FR, Luciw PA, Barr PJ, Varmus HE (1988) Characterization of ribosomal frameshifting in HIV-1gag-pol expression. Nature 331: 280–283

    Google Scholar 

  21. Johnson PR, Goldstein S, London WT, Fomsgaard A, Hirsch VM (1990) Molecular clones of SIVsm and SIVagm: experimental infection of macaques and African green monkeys. J Med Primatol 19: 279–286

    Google Scholar 

  22. Kikukawa R, Koyanagi Y, Harada S, Kobayashi N, Hatanaka M, Yamamoto N (1986) Differential susceptibility to the acquired immunodeficiency syndrome retrovirus in cloned cells of human leukemic T-cell line, Molt-4. J Virol 57: 1159–1162

    Google Scholar 

  23. Kuwata T, Igarashi T, Ido E, Minghao J, Mizuno A, Chen J, Hayami M (1995) Construction of HIV-1/SIVmac chimeric viruses havingvpr and/ornef of different parental origins and their in vitro and in vivo replication. J Gen Virol 76: 2181–2191

    Google Scholar 

  24. Lewis N, Williams J, Rekosh D, Hammarskjold ML (1990) Identification of a cis-acting element in human immunodeficiency virus type 2 (HIV-2) that is responsive to the HIV-1rev and human T-cell leukemia virus types I and IIrex proteins. J Virol 64: 1690–1697

    Google Scholar 

  25. Li J, Lord CI, Haseltine W, Letvin NL, Sodroski J (1992) Infection of cynomolgus monkeys with a chimeric HIV-1/SIVmac virus that expresses the HIV-1 envelope glycoproteins. J Acquir Immune Defic Syndrome 5: 639–46

    Google Scholar 

  26. Malim MH, Bohnlein S, Fenrick R, Le SY, Maizel JV, Cullen BR (1989) Functional comparison of the Rev trans-activators encoded by different primate immunodeficiency virus species. Proc Natl Acad Sci USA 86: 8222–8226

    Google Scholar 

  27. Miura T, Tsujimoto H, Fukasawa M, Ohta Y, Honjo S, Hayami M (1989) Genetic analysis and infection of SIVagm and SIVmnd. J Med Primatol 18: 255–259

    Google Scholar 

  28. Muesing MA, Smith DH, Cabradilla CD, Benton CV, Lasky LA, Capon DJ (1985) Nucleic acid structure and expression of the human AIDS/lymphadenopathy retrovirus. Nature 313: 450–458

    Google Scholar 

  29. Ohta Y, Masuda T, Tsujimoto H, Ishikawa K, Kodama T, Morikawa S, Nakai M, Honjo S, Hayami M (1988) Isolation of simian immunodeficiency virus from African green monkeys and seroepidemiologic survey of the virus in various non-human primates. Int J Cancer 41: 115–122

    Google Scholar 

  30. Ratner L, Haseltine WA, Patarca R, Livak KJ, Starcich B, Josephs SJ, Doran EK, Rafalski JA, Whitehorn EA, Baumeister K, Ivanoff SR, Petteway SR Jr, Pearson ML, Lautenberger JA, Papas TS, Ghrayeb J, Chang NJ, Gallo RC, Wong-Staal F (1985) Complete nucleotide sequence of the AIDS virus, HTLV-III. Nature 313: 277–284

    Google Scholar 

  31. Sakai H, Sakuragi J, Sakuragi S, Kawamura M, Adachi A (1993) Compatibility of Tat and Rev transactivators in the primate lentiviruses. Arch Virol 129: 1–10

    Google Scholar 

  32. Sakai H, Shibata R, Miura T, Hayami M, Ogawa K, Kiyomasu T, Ishimoto A, Adachi A (1990) Complementation of therev gene mutation among human and simian lentiviruses. J Virol 64: 2202–2207

    Google Scholar 

  33. Sakai H, Shibata R, Sakuragi J, Kiyomasu T, Kawamura M, Hayami M, Ishimoto A, Adachi A (1991) Compatibility ofrev gene activity in the four groups of primate lentiviruses. Virology 184: 513–520

    Google Scholar 

  34. Sakai H, Siomi H, Shida H, Shibata R, Kiyomasu T, Adachi A (1990) Functional comparison of transactivation by human retrovirusesrev andrex genes. J Virol 64: 5833–5839

    Google Scholar 

  35. Sakuragi S, Shibata R, Mukai R, Komatsu T, Fukasawa M, Sakai H, Sakuragi J, Kawamura M, Ibuki K, Hayami M, Adachi A (1992) Infection of macaque monkeys with a chimeric human and simian immunodeficiency virus. J Gen Virol 73: 2983–2987

    Google Scholar 

  36. Sanchez PR, Power MD, Barr PJ, Steimer KS, Stempien MM, Brown SS, Gee WW, Renard A, Randolph A, Levy JA, Dina D, Lucin PA (1985) Nucleotide sequence and expression of an AIDS-associated retrovirus (ARV-2). Science 227: 484–492

    Google Scholar 

  37. Shibata R, Kawamura M, Sakai H, Hayami M, Ishimoto A, Adachi A (1991) Generation of a chimeric human and simian immunodeficiency virus infectious to monkey peripheral blood mononuclear cells. J Virol 65: 3514–3520

    Google Scholar 

  38. Shibata R, Miura T, Hayami M, Ogawa K, Sakai H, Kiyomasu T, Ishimoto A, Adachi A (1990) Mutational analysis of the human immunodeficiency virus type 2 (HIV-2) genome in relation to HIV-1 and simian immunodeficiency virus SIVagm. J Virol 64: 742–747

    Google Scholar 

  39. Shibata R, Miura T, Hayami M, Sakai H, Ogawa K, Kiyomasu T, Ishimoto A, Adachi A (1990) Construction and characterization of an infectious DNA clone and of mutants of simian immunodeficiency virus isolated from the African green monkey. J Virol 64: 307–312

    Google Scholar 

  40. Shibata R, Sakai H, Kiyomasu T, Ishimoto A, Hayami M, Adachi A (1990) Generation and characterization of infectious chimeric clones between human immunodeficiency virus type 1 and simian immunodeficiency virus from an African green monkey. J Virol 64: 5861–5868

    Google Scholar 

  41. Tsujimoto H, Hasegawa A, Maki N, Fukasawa M, Miura T, Speidel S, Cooper RW, Moriyama EN, Gojobori T, Hayami M (1989) Sequence of a novel simian immunodeficiency virus from a wild-caught African mandrill. Nature 341: 539–541

    Google Scholar 

  42. Viglianti GA, Mullins JI (1988) Functional comparison of transactivation by simian immunodeficiency virus from rhesus macaques and human immunodeficiency virus type 1. J Virol 62: 4523–4532

    Google Scholar 

  43. Wain HS, Sonigo P, Danos O, Cole S, Alizon M (1985) Nucleotide sequence of the AIDS virus, LAV. Cell 40: 9–17

    Google Scholar 

  44. Willey RL, Smith DH, Laskey LA, Theodore TS, Earl PL, Moss B, Capon DJ, Martin MA (1988) In vitro mutagenesis identifies a region within the envelope gene of the human immunodeficiency virus that is critical for infectivity. J Virol 62: 139–147

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Research Center for Immunodeficiency Viruses, Institute for Virus Research, Kyoto University, Kyoto, Japan

    T. Miura, T. Kuwata, J. Chen, M. Jin, E. Ido & M. Hayami

  2. Department of Viral Oncology, Institute for Virus Research, Kyoto University, Kyoto, Japan

    R. Shibata & A. Adachi

Authors
  1. T. Miura
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. Shibata
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. Adachi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. T. Kuwata
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J. Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. M. Jin
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. E. Ido
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. M. Hayami
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Miura, T., Shibata, R., Adachi, A. et al. Genetic complementation between replication-defective mutants of HIV-1 and SIVagm. Archives of Virology 141, 31–41 (1996). https://doi.org/10.1007/BF01718586

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01718586

Keywords

Search

Navigation