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Strain-dependent differences in the human cytomegalovirus replication origin

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Summary

The nucleotide sequence of the human cytomegalovirus replication origin of strain Towne (anAatII-SacI fragment corresponding to nt 90372–94637 of strain AD169) was determined and compared with AD169. Two differences were found in the nucleotide sequence level. One was the alteration of structural organization (a major difference): a 189-bp region of AD169 (nt 93337–93525) was directly repeated three times in Towne. The other was a change in the nucleotide residue level including substitution, insertion, or deletion (a minor difference). The divergent residues were predominantly localized within the nt 92591–92855 region of AD169. A replication assay revealed that replication ability remained after deletion of the 189-bp repeat but disappeared after either a 1.5-kb deletion from theAatII end or a 0.9-kb deletion from theSacI end. The 1.5- and 0.9-kb regions were relatively conserved. These results indicate that at least two regions essential for replication ability lie outside of both the relatively variable region and the 189-bp repeat and suggest that these essential regions support replication even with a spatial separation of either one (AD169) or three repeats (Towne) of the 189-bp region.

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  1. Department of Virology, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan

    Z. Chen, S. Watanabe & N. Yamaguchi

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  1. Z. Chen
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  2. S. Watanabe
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  3. N. Yamaguchi
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Chen, Z., Watanabe, S. & Yamaguchi, N. Strain-dependent differences in the human cytomegalovirus replication origin. Archives of Virology 141, 13–30 (1996). https://doi.org/10.1007/BF01718585

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  • DOI: https://doi.org/10.1007/BF01718585

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