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Restriction endonuclease mapping and molecular cloning of the human herpesvirus 6 variant B strain Z29 genome

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Summary

Human herpesvirus 6 (HHV-6) variants A and B differ in cell tropism, reactivity with monoclonal antibodies, restriction endonuclease profiles, and epidemiology. Nonetheless, comparative nucleotide and amino acid sequences from several genes indicate that the viruses are very highly conserved genetically. The B variant is the major etiologic agent of exanthem subitum and is frequently isolated from children with febrile illness; no disease has been etiologically associated with HHV-6A. One HHV-6A strain has been cloned and sequenced, but similar information and reagents are not available for HHV-6B. We report here the determination of maps of the restriction endonuclease cleavage sites forBamHI,ClaI,HindIII,KpnI, andSalI, and the cloning in plasmids and bacteriophages of fragments representing over 95% of the HHV-6B strain Z29 [HHV-6B(Z29)] genome. Hybridization experiments and orientation of several blocks of nucleotide sequence information onto the genomic map indicate that HHV-6A and HHV-6B genomes are colinear.

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Author information

Author notes

  1. T. R. Dambaugh

    Present address: H. L. Yoh Co., Newark, DE

  2. R. M. Danovich

    Present address: Schering-Plough Research Institute, Kenilworth, NJ

  3. N. Inoue

    Present address: National Institute of Health, Tokyo, Japan

  4. N. Frenkel

    Present address: Tel Aviv University, Tel Aviv, Israel

Authors and Affiliations

  1. Department of Biology, Rhodes College, Memphis, Tennessee

    G. J. Lindquester, R. D. Allen & J. W. Castelli

  2. Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia

    N. Inoue, R. D. Allen, F. R. Stamey & P. E. Pellett

  3. DuPont Merck Pharmaceuticals, Wilmington, Delaware

    T. R. Dambaugh & J. J. O'Brian

  4. National Institutes for Health, Bethesda, Maryland, USA

    R. M. Danovich & N. Frenkel

Authors
  1. G. J. Lindquester
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  2. N. Inoue
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  3. R. D. Allen
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  9. N. Frenkel
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  10. P. E. Pellett
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Lindquester, G.J., Inoue, N., Allen, R.D. et al. Restriction endonuclease mapping and molecular cloning of the human herpesvirus 6 variant B strain Z29 genome. Archives of Virology 141, 367–379 (1996). https://doi.org/10.1007/BF01718406

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  • DOI: https://doi.org/10.1007/BF01718406

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